Soil sample metagenome NGS data management for forensic investigation

Soil analysis is a valuable resource in forensic investigation. Classical forensic soil analysis involves examination of its physical characteristics and chemical composition, such as soil type, colour, particle size, shape, pH, elemental, mineral and organic content. However the limited variability of these parameters is not always allowing adequate discrimination between soil samples. As soil supports extreme diversity of microorganisms and eukaryotic communities, microbiological approaches have been proposed. Several molecular approaches for microbial DNA profiling are available; however there is a lack of published data of implementation of the next generation sequencing (NGS) approaches for forensic soil analysis.The aim of the current study was elaboration of criteria for soil metagenome data management and database searching. We used our previously sequenced collection of 11 samples collected from different environments (forests, fields, grasslands, urban park) with different flora. The single sample collection includes 9 soil samples per one sampling area (30 m × 30 m) spaced by 15 m. In the current study we concentrated mainly on 18S rRNA gene V2-V3 region for fungi however SSU rRNA region for arbuscular mycorrhizal (AMF) fungi and V2-V3 hypervariable region of 16S rRNA gene for bacterial communities were taken into account. The sequencing was performed by Roche/454 platform. For data analysis OTU based approach on mothur software and NCBI BLASTN search were used. NCBI BLASTN analysis revealed altogether 2983 AMF matches and 8997 18S matches as well as 25477 OTUs (16S) were determined. Several data filtration approaches were used for data management. We found that 18S marker results could be used to create and run a filtered database that is computationally much more efficient and flexible. Our results have broad impact; however more samples have to be analysed, additional studies performed and cooperation between soil scientists and forensic scientists is required to be able to implement these novel techniques into the routine forensic practice.     

Multiplex high resolution melt (HRM) messenger RNA profiling assays for body fluid identification

Traditional body fluid identification methods use a variety of technologically diverse techniques that do not permit the identification of all body fluids. Definitive identification of the biological material present can be crucial to a fuller understanding of the circumstances pertaining to a crime. Thus definitive molecular based strategies for the conclusive identification of forensically relevant biological fluids need to be developed. Messenger (mRNA) profiling is an example of such a molecular based approach.Current mRNA body fluid identification assays typically involve either capillary electrophoresis (CE) or quantitative RT-PCR (qRT-PCR) platforms, each with its own limitations. Both platforms require the use of expensive fluorescently labeled primers or probes. CE-based assays require separate amplification and detection steps thus increasing the time required for analysis. For qRT-PCR assays, only 3 or 4 markers can be included in a single reaction since each requires a different fluorescent dye. To simplify mRNA profiling assays and to reduce the time and cost of analysis, we have developed multiplex high resolution melt (HRM) assays that provide an identification of all forensically relevant biological fluids and tissues.     

The efficiency of Y-chromosome markers in forensic trace analysis and their inclusion in the Austrian National DNA Database

Y-STR markers are a valuable tool in the analysis of biological traces in which a mixture of male and female trace material is to be expected. It is possible to generate a Y-chromosome DNA profile, even if all the prior sperm tests are negative and no sign of any male component is found in amelogenin. In 38 of a total of 239 sexual offences a perpetrator trace was identified solely using Y-STR analysis. Based on these findings, the Austrian National DNA Database was expanded to include Y-STRs in 2012 with the primary objective to identify serial sexual offences.     

Book reviews

Making Sense in Law: Linguistic, Psychological and Semiotic Perspectives, BERNARD S. JACKSON Liverpool: Deborah Charles Publications 1995

Culture And Sexual Risk: Anthropological Perspectives On Aids, HAN T. BRUMMELHUIS &; GILBERT HERDT (Eds) Amsterdam: Gordon and Breach Publishers, 1995

Virtual Geography, MCKENZIE WARK, Bloomington: Indiana University Press, 1994     

The Defining Elements of Advocacy Coalitions: Continuing the Search for Explanations for Coordination and Coalition Structures

As the theoretical and practical interest in policy networks increases, so does the need for further research into how, and based on what rationales, actors within a policy subsystem engage in interorganizational collective action and form political coalitions. The aim of this paper is to continue the search for explanations for coordination and coalition structures in the setting of Swedish carnivore policy. Based on the Advocacy Coalition Framework (ACF) and a previous case study within the same policy subsystem, the study investigates a set of hypotheses regarding actors' coordinating behavior and the defining elements of coalitions. The empirical analysis indicates, in support of the ACF, that perceived belief correspondence is a better predictor of coordination than perceived influence. Moreover, the explanatory power of empirical policy core beliefs in general, and normative policy core beliefs in particular, is further reinforced, while deep core beliefs seemingly do not influence coalition structure. The relevance of more shallow beliefs for coalition formation cannot be dismissed and therefore calls for additional research.     

Solitary Sunnah and Its Legislative Value Among the Muslim Jurists： Readings of Selected Examples

The science of hadith authentication and classification （ulum al-hadith） has been developed by Muslim scholars mostly based on critical analysis of the texts and chains of the reports （ahadith） that are attributed to Prophet Muhammad （s.a.w.）. Though this science is basically designed to identify the authentic reports which are truly the words, actions or tacit approvals of the Prophet （s.a.w.）, however, it has left its implications onfiqh （legal） opinions among the Muslim jurists （fuqaha）, who left contrasting legal opinions on certain issues offiqh, some of which are as a result of their differences on hadith classification, particularly classifications of hadith into solitary and recurrent reports. This paper uses analytical method, and intends to address the implications of hadith authentication and classification methods on the legislative power of the sunnah, with particular emphasis on the solitary sunnah and its legislative value among the Muslim jurists.     

胡锦涛的团结观论析

胡锦涛的团结观建构在马克思主义团结观的基础之上,是对毛泽东、邓小平、江泽民团结思想的继承和发展,是凝聚人心、完成执政使命的必然。从"斗争思维"向"合作思维"的转变是胡锦涛团结观的逻辑前提,胡锦涛重视团结意识的强化,主张"建设和谐社会",用中国特色社会主义伟大事业凝聚人心、增进共识、促进团结。当下,胡锦涛的团结观有着重要的启示。     

我国生产者责任延伸制度实施困境分析——从家电以旧换新政策实施谈起

生产者责任延伸是解决废弃产品问题的一项新兴的环境策略,在我国已初步构建并开始实施。为拉动经济增长,促进废旧电器电子产品的循环利用,我国实施了家电以旧换新政策。但该政策实施后遇到的一些问题,如回收企业技术落后不能满足回收的大量废弃电器电子产品的无害化资源化处置、生产者组织和废弃产品处理基金制度没有有效建立、政府行政干预机制和经济激励机制的缺位、法律规定的空白等阻碍着生产者责任延伸制度的实施。     

Hong Kong's Elite Structure,Legislature and the Bleak Future of Democracy under Chinese Sovereignty

A decade after retrocession to China, Hong Kong still has limited democracy. Only half of the legislature is directly elected by the people, and the Chief Executive – the leader of Hong Kong government – is chosen by an election committee of 800 people, most of them loyal to Beijing. In December 2007, the Chinese Central Government announced that the direct election of the Chief Executive and the whole legislature may be implemented from 2017 and 2020, respectively. Still, in this paper it is suggested that real democracy in the foreseeable future is unlikely. This paper demonstrates the existence of a “power elite” of tightly knit business-state networks in Hong Kong society and argues that this constitutes a systemic barrier against further democratic development. Central to the discussion is an analysis of the formation and composition of the legislature and the relations to the elite-interlocks among influential organisations in society. Mainly based on 2006‐07 data, the Elite Database which is subject to network analyses is composed of 1531 individuals holding 1854 seats from 40 listed corporations, 27 government committees, 20 non-governmental organisations, and eight universities.     

Innovation and growth in traditional agriculture of the lower Volta,Ghana

This article addresses the important question of whether foreign direct investment enhances economic growth and labour productivity in Mexico, both from a theoretical and empirical perspective. After briefly reviewing the Mexican experience with net FDI inflows during the 1990s, the article presents a simple endogenous growth model which explicitly incorporates any positive (negative) externalities generated by additions to the foreign capital stock. Using cointegration analysis, the article estimates a dynamic labour productivity function for the 1960-95 period that includes the impact of the growth rate in the stocks of both private and foreign capital (as opposed to the flows) and the economically active population (EAP) (rather than the rate of population growth). The error correction model (ECM) estimates suggest that increases in both private ad (lagged) foreign investment spending, as well as the rate of growth in exports, have a positive and economically significant effect on the rate of labour productivity growth. In addition, the results show that increases in the EAP have a negative and statistically significant effect on the rate of labour productivity growth, while changes in the government consumption variable have a negative but marginally significant impact. The error correction terms of the estimated models are negative and statistically significant, thus suggesting that deviations of actual labour productivity growth from its long-run value are corrected in subsequent periods. Finally the article generates historical simulations from the estimated ECM's and offers some policy recommendations to enhance the positive externalities associated with FDI inflows.