桃红四物汤中5种主要成分在脑缺血再灌注模型大鼠体内组织分布的研究

目的 研究脑缺血再灌注模型大鼠在灌胃桃红四物汤(Taohong Siwu Decoction, THSWD)后,5种主要活性成分(藁本内酯、苦杏仁苷、芍药苷、羟基红花黄色素A、阿魏酸)在体内组织分布的差异和规律。方法 采用线栓法构建大鼠大脑中动脉闭塞(middle cerebral artery occlusion, MCAO)模型,然后再灌注24 h建立脑缺血再灌注大鼠模型;将模型复制成功的大鼠灌胃THSWD(50 g/kg)后,于不同时间点采集组织(心、肝、脾、肺、肾、脑),样品处理后采用UPLC-MS技术检测各组织中待测成分的含量。结果 各成分在模型大鼠各组织中分布广泛,在肝、肾组织中浓度较高,其中藁本内酯在脑组织中有较高浓度;苦杏仁苷在肺部具有组织分布特异性;芍药苷、羟基红花黄色素A在大鼠肝、脾、肺组织具有较高分布比例,阿魏酸在脑组织中未检出。结论 THSWD中5种主要活性成分在各组织中分布广泛,各组织不同时间点的分布趋势也存在较大差异。     

胶体金标记检测大麻单克隆抗体免疫试剂盒研制

目的 建立准确、快速、简便的检测尿液中大麻的胶体金免疫层析技术(ICT)。方法 采用柠檬酸三钠还原法制备胶体金颗粒,标记抗主要代谢物四氢大麻酚-9-羧酸,THC与GC/MS检测方法相比,用ICT法的216份尿样,其检测限为50 ng/ml,灵敏度为96.67％,准确性为98.61％。结论 ICT法检测尿液中THC,其特异性强,对确定大麻的存在具有广泛的应用价值。     

乳酸菌对子宫内膜上皮细胞的黏附及其对致病菌的拮抗作用

以永生化山羊子宫内膜上皮细胞作为体外模型,采用革兰氏染色法观察乳酸菌和致病菌对子宫内膜上皮细胞的黏附特性;通过平板计数法观察乳酸菌对致病菌的黏附拮抗作用.结果显示,嗜酸乳杆菌、粪肠球菌、盲肠肠球菌对子宫内膜上皮细胞均表现出很强的黏附特性,黏附细菌数分别为每个细胞87、45、34个.黏附拮抗试验显示,3株乳酸菌均使致病菌...     

新藤黄酸体外抑制肺腺癌A549细胞增殖的作用

目的 研究新藤黄酸(Gambogenic acid,GNA)抑制肺腺癌细胞株A549增殖的作用及其相关机制.方法 不同浓度GNA分别作用A549细胞24、48和72 h,甲基噻唑基四唑(methyl thiazolyl tetrazolium,MTT)比色法检测药物对A549细胞增殖的抑制率,吖啶橙/溴化乙啶(acri...     

王枣子乙素聚乳酸纳米粒的制备与质量评价

目的 对王枣子乙素聚乳酸纳米粒制备工艺和含量测定方法进行研究,并对其进行质量评价.方法 用自乳化溶剂扩散法制得王枣子乙素聚乳酸纳米粒,并对其粒径、形态、表面电位、包封率等进行研究.结果 所得王枣子乙素聚乳酸纳米粒粒径分布均匀,平均粒径为(120±10.6)nm,Zeta电位为-17.6 mV,平均包封率为(90.38±...     

Alcohol dependence and criminal behavior: preliminary results of an association study of environmental and genetic factors in an italian male population

The aim of this study is to propose an innovative approach evaluating the connection between alcohol use disorders and criminal behavior. The research, structured as a case–control study, was based on the analysis of environmental (social variables) and genetic factors (single nucleotide polymorphisms of glutamic acid decarboxylase) in a population (N = 173) of Italian alcohol‐dependent men. Group 1 (N = 47, convicted subjects) was compared with Group 2 (N = 126, no previous criminal conduct). Grade repetition, work problems, and drug problems were statistically associated with criminal behavior. Having daily family meals together and having children were inversely related to convictions. The genotype distribution of the two groups was similar. The association between environmental factors and antisocial behavior confirms previous findings in the literature. The lack of genetic association does not exclude the role of the gamma‐aminobutyric acid (GABA) system in determining antisocial behavior; further studies with larger samples are needed, together with investigation of other components of the GABA pathway.     

氟乙酰胺类杀鼠药检测方法的研究进展

综述了氟乙酸、氟乙酰胺、氟乙酸钠的检测方法,通过比较发现存在多种柱前衍生化途径,其中采用五氟卞基溴(PFBBr)柱前衍生化进行GC/ECD检测的灵敏度最高检测限可达1.0ng/mL,而气相质谱联用仪、液相质谱联用仪通过子母离子对的检测可进一步确定衍生化产物,离子色谱和毛细管电泳检测可减少衍生化步骤直接对样品中的氟乙酸钠进行检测,但响应值不如气相色谱。     

GHB free acid: II. Isolation and spectroscopic characterization for forensic analysis

A reference standard for gamma-hydroxybutyric acid (GHB) free acid is not commercially available, making its analysis in forensic exhibits more difficult. GHB free acid is typically encountered in aqueous solution and in the presence of the lactone, gamma-butyrolactone (GBL), presenting difficulty in Fourier transform infrared (FT-IR) analysis. The strong infrared (IR) absorptivity of the GBL carbonyl band, the shifting of the GBL carbonyl band in aqueous solutions, and the position of the O-H bend for water can mask the main carbonyl band for GHB free acid. Model solutions of beta-hydroxybutyric acid (BHB) and GBL were studied in order to further understand the masking of the GHB free acid carbonyl band in FT-IR analysis. The use of second derivative FT-IR spectroscopy was shown to provide resolution of the free acid carbonyl band, and a presumptive test for GHB free acid was developed and applied. An extension of this work included preparing, for use as a standard reference material, small amounts (< or = 10 mg) of GHB free acid. Preparation was based on the instantaneous reaction of GHB's sodium salt with a stoichiometric amount of hydrochloric acid in aqueous solution, and subsequent isolation of the free acid in neat liquid form. Both FT-IR and proton nuclear magnetic resonance spectra of the neat reference material were obtained and used to verify its identity. The isolation of GHB free acid from actual forensic exhibits is also presented, with identity confirmation using FT-IR.     

毛发中GHB的检测及评价

目的探索毛发中外源性GHB的检测及判断的可行性,为涉GHB的鉴定提供方法和依据。方法建立毛发中GHB的GC/MS分析方法,并通过动物实验,考察毛发中内源性GHB的质量分数范围、外源性GHB在毛发中的时间过程以及给药剂量、毛发颜色与毛发中GHB的质量分数关系。结果豚鼠和中国人黑色毛发中内源性GHB质量分数分别为(3.01±1.41)ng/mg(n=28)和(1.02±0.27)ng/mg(n=20);摄GHB后毛发中GHB质量分数明显增加且与给药剂量呈正相关性;GHB在毛干中呈窄带分布;深色毛发中GHB质量分数高于浅色毛发。结论毛发中GHB的检测适用于GHB滥用和中毒的法医毒物学鉴定;根据毛发中的GHB质量分数和毛发分段分析可判断GHB的来源。