职工群众工作方法论

新时期的职工群众工作,已呈现出诸多不同以往的新特点,一是工作对象更加广泛;二是工作内容更加多元;三是工作环境更加复杂;四是健全组织网络难度不断增大。各级工会干部要悉心研究工作方法,以适应时代变化对工会工作带来的新需求。     

Simplicity and Spook: Terrorism and the Dynamics of Threat Exaggeration

It has been common, at least since 1945, to exaggerate and to overreact to foreign threats, something that seems to be continuing with current concerns over international terrorism. This paper sketches threat exaggeration during the Cold War and applies the experience from that era to the current one. Alarmism and overreaction can be harmful, particularly economically. And, in the case of terrorism, it can help create the damaging consequences the terrorists seek but are unable to perpetrate on their own. Moreover, many of the forms alarmism has taken verge on hysteria. The United States is hardly "vulnerable" in the sense that it can be toppled by dramatic acts of terrorist destruction, even extreme ones. The country can, however grimly, readily absorb that kind of damage, and it has outlasted considerably more potent threats in the past.     

荧光标记复合扩增检测4个STR基因座多态性

目的建立荧光标记复合扩增D1S2142,D13S1492,D14S306,D15S659基因座检测分型方法,并对成都汉族群体4个基因座的遗传多态性进行调查。方法用6-FAM标记D1S2142和D15S659引物,HEX、TMR分别标记D14S306和D13S1492引物,PCR复合扩增,310基因分析仪电泳自动收集电泳结果数据,GeneScan Analysis Software3.7NT软件计算扩增产物片段相对大小,Genotyper(3.7NT软件进行样本基因型分型,建立了荧光标记复合扩增检测4个STR基因座基因型的方法,对145名成都汉族无关个体样本进行分型。结果荧光标记复合扩增D1S2142,D13S1492,D14S306,D15S659基因座,每个STR基因座都获得了清晰的基因型分型结果。145份样本,4个STR基因座分别检出10,14,7,12个等位基因和22,54,21,39种基因型,其基因型分布均符合Hardy-W e inberg平衡。4个基因座在成都汉族群体的杂合度分别依次为0.7793,0.8345,0.7793和0.8345;多态信息含量分别依次为:0.7656,0.8730,0.7470和0.8312。累计非父排除率为0.9783,累计个人识别机率为0.9999 917。结论荧光标记复合扩增D1S2142,D13S1492,D14S306,D15S659基因座,可实现对每个基因座准确分型;成都汉族群体该4个基因座的遗传学数据,可为群体遗传学和法医学研究与应用提供基础资料。     

高效液相色谱-质谱法测定人尿液中乙基葡萄糖醛酸苷

目的建立高效液相色谱-质谱法测定人尿液中乙基葡萄糖醛酸苷。方法取空白人尿液加入EtG-D5内标工作液和冷甲醇（4℃）,经涡旋、离心,取上清液于水浴氮气吹干,以沉淀杂质,采用Agilent Zorbax Bonus-RP色谱柱分离,以乙腈∶0.1%甲酸水溶液（5∶95）为流动相,采用负离子模式质谱检测,在选择离子监测模式下进行定性和定量测定。结果尿液中EtG在50～5 000ng/mL范围内线性关系良好,检出限为18ng/mL,方法回收率为90.2%～101.7%,相对标准偏差小于10%。结论所建方法简便,具有较高的回收率和精密度,适用于尿液中EtG的检测。     

中央苏区时期反腐败斗争中的群众监督

中华苏维埃政府曾被号称＂空前的真正的廉洁政府＂,中央苏区时期,反腐败斗争取得卓越的成绩与群众的监督有着密切的联系。当时,群众参与监督的方式主要有：通过建立民主制度监督;群众参与监督机构;发动群众进行检举和控告;诉诸舆论武器。中央苏区时期反腐败工作发挥群众监督的作用对今天仍有许多启示。     

对马克思主义大众化的三维解读

在当代中国，必须从理论、信仰、实践三个维度理解、解读马克思主义大众化。只有这样，才能推动当代中国马克思主义大众化。     

示范诉讼制度探析——兼论我国代表人诉讼制度之完善

示范诉讼制度具有其他群体性纠纷解决制度所不具备的特征和独特功能,正因如此,目前已经有许多国家和地区在民事诉讼立法中对其加以明确规定,还有些国家在实践中大量运用这一制度以解决实际问题。我国现行民事诉讼法有关群体性纠纷解决制度的规定存在明显的缺陷,结合在新形势下我国所面临的司法现实需求,为了更好的解决群体性纠纷,在我国未来的民事诉讼立法当中,在对代表人诉讼制度进行完善的同时,应当引入示范诉讼制度;同时考虑到示范诉讼自身的一些特点,在具体制度设计上,要充分考虑并注意处理好其与代表人诉讼制度的协调问题,以便更好地发挥两种制度各自的优点。     

Detection of Gamma-Hydroxybutyric Acid in Various Drink Matrices via AccuTOF-DART*

Abstract: A new screening method for detecting gamma-hydroxybutyric acid (GHB) in drink matrices, using the IonSense, Inc. (Saugus, MA) direct analysis in real time (DART) ion source coupled to a JEOL exact mass time-of-flight mass spectrometer (AccuTOF), was validated and compared with the current screening methodology. The DART ion source allows for analysis of samples under ambient conditions with little to no sample preparation. Fifty drink specimens were spiked at levels of 1, 2, 3, and 4 mg/mL GHB, and analyzed on the AccuTOF-DART. Positive detection of GHB occurred for each of the samples at each concentration level, giving 100% accuracy for the samples tested. Twenty-five of the 50 drink specimens were spiked at 1 mg/mL GHB and tested using a color test known as the GHB Color Test #3. Only two of these 25 specimens tested positive for the presence of GHB, giving only 8% accuracy. Implementation of this new methodology as a screening tool for GHB analysis will quickly eliminate negative specimens allowing the examiner to focus analysis time on those that screened positive.     

坚持党的群众路线强化党的核心凝聚力

党的十八大以来，群众工作是我党在新的历史时期保障我国社会转型顺利进行的一项基础性工作。随着社会矛盾不断激化、社会冲突性事件频发、党群关系恶化，我党的核心凝聚力及社会凝聚力不断下滑，社会稳定和发展受到较大的影响和阻碍。新历史时期下，社会凝聚力建设是我国社会转型的重要任务。只有进一步深化发展党的群众路线，密切联系群众，才能确保我党和国家的进一步繁荣和发展。     

嗜尸性苍蝇mtDNA中COⅡ基因序列检测

目的利用线粒体DNA(m tDNA)上细胞色素氧化酶辅酶Ⅱ(COⅡ)中635bp基因序列,解决嗜尸性苍蝇及其卵和幼虫种类鉴定的难题。方法随机采集放置在呼和浩特地区室外草地家兔尸体上的嗜尸性苍蝇、幼虫、苍蝇腹中的卵。利用Chelex方法提取上述苍蝇m tDNA;通过Perk in-E lm er 9600扩增仪进行PCR扩增;琼脂糖水平电泳和银染显色技术进行扩增结果检测;PCR胶回收试剂盒纯化;AB I 377测序仪测序;DNAMAN 4.0序列分析软件,进行序列比对,截取等长度片段;MEGA2.1软件包进行序列分析和构建系统发育树。结果上述嗜尸性苍蝇m tDNA上COⅡ基因序列在双翅目嗜尸性苍蝇的种内差异均数小于1%,种间差异均数大于3%,成虫与幼虫、卵无明显差异。以此能够根据COⅡ序列差异判断两个个体是否同种。然而,对于亲缘关系非常接近的铜绿蝇和丝光绿蝇来说,由于二者的种内、种间进化分歧均数非常接近,运用上述两个片段则很难区别。结论m tDNA上COⅡ序列分析能有效地对绝大多数嗜尸性苍蝇进行种类鉴定。该检测方法快速、简便和精确,能作为法医鉴别嗜尸性苍蝇种类的依据。