Rh血型系统的分子生物学研究进展

Rh血型是极复杂的人类红细胞血型系统之一,至少包括45种独立的血型抗原。过去的10年间,Rh血型的研究取得了极大的进展,得到了大量分子生物学的研究资料,如Rh血型的遗传方式、RH基因及其进化过程、Rh复合体的结构与功能、红细胞外Rh相似蛋白的表达,Rh基因分型技术也进一步得到发展与改进。本文综述了Rh血型分子生物学的研究进展,以服务于法医学实践。     

Forced displacements in mining communities: politics in Chiadzwa diamond area,Zimbabwe

ABSTRACT

The Chiadzwa diamonds attracted widespread attention due to human rights violations and illegal smuggling. When diamonds were discovered in 2006, thousands of artisanal miners descended on the diamond fields. In response, the government unleashed the army and police in brutal crackdowns to drive artisanal miners off the diamond fields. This militarisation of diamond fields and extraction was followed by forced displacement of the Chiadzwa people. This article examines the lived, everyday experiences of the displaced Chiadzwa people. Findings reveal that displacements dislocated the livelihoods and socialities of the people. Displacements also exacerbated people's vulnerability to livelihood shocks, insecurity, and poverty. In relocating people the government adopted a ‘top-down’ approach which triggered contestations and conflicts with the people who felt alienated from their ancestral land and excluded from diamond wealth. Consequently, sabotage, resistance and subversion were commonplace in the relocation process. These socio-political ‘tactics’ should be viewed as ‘weapons of the weak’.     

“Clamoring Blood”: The Materiality of Belonging in Modern Ainu Identity

The tension between silence and vocalization, embrace and rejection, of Ainu ancestry has been a key factor in negotiating Ainu subjectivity since Ainu territories were colonized in 1869. As early as 1799, expressions of Ainu ethnicity were alternately cloaked and exaggerated as Japan vacillated between assimilation and segregation policies in eastern Hokkaido Ainu communities. Officially recognized as Japan's indigenous peoples in 2008, Ainu subjectivity has become increasingly politicized as the state and other stakeholders seek to define Ainu ethnicity for future legislation. Today Ainu belonging is frequently gauged by bodily metaphors of a vocalized blood. Cultural sensibility and blood are often conflated in Ainu discourses of identity: Ainu revivalists report that a sensation of “clamoring blood” (J: chi ga sawagu) inspires them to revisit ancestral memories and begin fashioning Ainu identities. Historically, intra-Ainu relations were not bound to blood but instead embodied in material expressions, such as invisible cords for women and crest-like emblems for men, symbols that enabled flexibility where needed. Since the twentieth century, the hyper focus on blood raises the specter of colonially imposed rhetorics of eugenics, assimilation policies, and specifically, the problem of race. Relatedness in the Ainu community is not exclusively defined by “consanguineal relations”; rather, a long history of adopting ethnic Japanese children and non-Ainu into Ainu families renders complex the question of identity. This article assesses how immutable notions of racial difference intersect with self-determination and current articulations of Ainu identity.     

Ecologies of blood in Johannesburg vampire fiction

This essay reads two contemporary Johannesburg vampire narratives, the film Eternity (2010) and the graphic novel Rebirth (2012), as part of a body of fiction that sees post-apartheid Johannesburg as a laboratory for new forms of life. While recent Johannesburg science fiction has imagined the emergence of new and more fluid forms of being in Johannesburg, the vampire narrative employs a far older and more restrictive type of monster. By virtue of its intimate relationship to blood – with its mythical overtones of racial and national essence – and its peculiarly blank and limited form of being, the vampire is a productive figure for imagining whiteness. In this essay, I examine how the Johannesburg vampire registers the mutations of whiteness in the post-apartheid context. Located in a contaminated and contaminating city of hidden capillaries and networks, vampiric blood runs across and perforates the traditional blood bonds of race, family, and nation. Indeed, dependent on a precarious and potentially diseased resource, the Johannesburg vampire is always on the cusp of dissolving the very whiteness it figures. I am interested in the way in which these vampire narratives alternately reinforce and interrogate what I call the politics of prolongation; that is, in the ways in which their horizontal and entangled “haemopoetics” attempt to displace the genealogical poetics of blood that have traditionally suffused the colonial enterprise and its literature.     

Determination of Nitrite in Whole Blood by High‐Performance Liquid Chromatography with Electrochemical Detection and a Case of Nitrite Poisoning

Although nitrite is widely used in meat processing, it is a major toxicity hazard to children and is responsible for the blue‐baby syndrome. A simple and effective method to determine nitrite in whole blood has been devised using ion chromatography with suppressed conductivity detection. The blood sample was deproteinized by adding acetonitrile and purified with mini‐cartridges to remove hydrophobic compounds, chloride ions, and metal ions. An aliquot of the filtrate was injected onto the ion chromatography. The retention time for nitrite was 13.8 min and the detection limit of nitrite in whole blood was 0.4 μmol/L. The calibration curve was linear (r² = 0.9999) over the concentration working range. The blood nitrite concentration of a victim who attempted suicide by ingesting sodium nitrite powder was determined using the present method. The basal levels for nitrite in human blood was determined with 7.1 ± 0.9 μmol/L (n = 12).     

Assessment of the Role Played by N‐propanol Found in Postmortem Blood in the Discrimination Between Antemortem Consumption and Postmortem Formation of Ethanol Using Rats

This study disproves the reliability of n‐propanol as a biomarker to establish whether the ethanol found in postmortem blood is derived from antemortem ingestion or postmortem putrefactive processes. Two groups of rats were given ethanol or normal saline solution, respectively, and sacrificed 1.5 h later. After putrefaction, blood and, in a few cases, urine samples from the rats were analyzed for ethanol and n‐propanol by head‐space gas chromatography equipped with flame ionization detection. Although the concentration ratios of ethanol/n‐propanol in the postmortem blood collected from the bodies without prior alcohol consumption were expected to be <20 (as per limited case reports and previous in vitro studies), in samples from several rats that were on saline solution, this ratio was found to exceed 20. In conclusion, the concentration ratio of ethanol/n‐propanol in postmortem blood does not allow for the discernment between antemortem ingestion and the postmortem synthesis of ethanol.     

Popliteal Vein Blood Sampling and the Postmortem Redistribution of Diazepam,Methadone, and Morphine

Postmortem redistribution (PMR) refers to the site‐ and time‐related blood drug concentration variations after death. We compared central blood (cardiac and subclavian) with peripheral blood (femoral and popliteal) concentrations of diazepam, methadone, and morphine. To our knowledge, popliteal blood has never been compared with other sites. Intracardiac blood (ICB), subclavian blood (SB), femoral blood (FB), and popliteal blood (PB) were sampled in 30 cases. To assess PMR, mean concentrations and ratios were compared. Influence of postmortem interval on mean ratios was also assessed. Results show that popliteal mean concentrations were lower than those for other sites for all three drugs, even lower than femoral blood; mean ratios suggested that the popliteal site was less subject to PMR, and estimated postmortem interval did not influence ratios except for diazepam and methadone FB/PB. In conclusion, our study is the first to explore the popliteal site and suggests that popliteal blood is less prone to postmortem redistribution.     

液相色谱-质谱联用法测定人全血中灭多威

目的建立一种简便的测定人全血中灭多威的液相色谱-质谱联用法。方法样品处理采用液-液乙酸乙酯萃取方法。色谱柱为Zorbax SB-C18(2.1mm×50mm,5μm),流动相为乙腈-0.1%甲酸,梯度洗脱,流速为0.5mL/min,柱温40℃。采用ESI离子源,MRM离子方式监测。结果灭多威在0.05~2.0μg/mL浓度范围内线性良好(r0.995)。灭多威的方法回收率均在90%~108%的范围内,日内、日间RSD均小于15%。结论本方法可简单、高效地检测全血中灭多威浓度。     

Examination of acetone findings in suspected drug-facilitated sexual assaults: A case series

Acetone presence in human biological specimens can result from exogenous administration or endogenous production, resulting from diabetes, dietary composition, alcoholism, and stress response. Victims of drug-facilitated sexual assaults (DFSA) are understood to experience enhanced stress. At the Harris County Institute of Forensic Sciences (HCIFS), DFSA drug testing includes analysis of volatile compounds, ethanol, methanol, isopropanol, and acetone, by headspace gas chromatography/flame ionization detection. The prevalence of acetone-positive specimens in DFSA casework has been observed to exceed that of other human performance case types. In this report, DFSA cases received between 2019 and 2021 (n = 393) were reviewed and 41 acetone-positive cases were detailed. Overall, nearly 11% of the DFSA cases had acetone-positive blood or urine specimens, where 3% identified acetone only, 6% identified acetone and other drug(s), and 2% identified acetone, ethanol, and other drug(s). Acetone concentrations ranged from 0.010 to 0.147 g/100 mL in urine. Other drugs such as nor-carboxy-Δ⁹-tetrahydrocannabinol, amphetamine, methamphetamine, ethanol, and benzoylecgonine were commonly detected. Elevated stress response encountered during DFSAs may facilitate the mechanism behind enhanced acetone production leading to increased identification. Limited availability of victim medical history precludes understanding the contribution of other disease states or physiological conditions. Nonetheless, the identification of acetone in DFSA specimens supports its potential as a biomarker of trauma in forensic toxicology casework and warrants future research within the community.