吉他霉素在猪体内的药代动力学特征及残留检测方法的研究

对7头健康猪按随机交叉设计经单剂量(2.5×104U/kg)内服吉他霉素原药及其微囊制剂进行了药代动力学试验。血浆样品经磷酸氢二钾溶液碱化、无水乙醚提取后,采用HPLC法测定血药浓度。结果表明,药时数据均符合一级吸收二室开放模型,主要药代动力学参数为:原药t1/2ka=0.23h±0.05h,t1/2β=6.43h±2.38h,tmax=0.65h±0.17h,Cmax=6.97μg/mL±1.40μg/mL,AUC=19.98mg/(L.h)±8.16mg/(L.h);微囊制剂t1/2ka=0.55h±0.16h,t1/2β=10.63h±3.73h,tmax=1.25h±0.23h,Cmax=7.97μg/mL±1.63μg/mL,AUC=36.24mg/(L.h)±6.15mg/(L.h)。吉他霉素原药在猪体内吸收较快,但其微囊制剂分布更广泛并消除较缓慢。对猪肌肉、肝和肾等添加吉他霉素标样后均采用0.2%偏磷酸-甲醇萃取后过SCX小柱用甲醇抽提吹干,脂肪加标样采用正己烷除脂后用甲醇反萃取减压蒸干。采用HPLC法测定组织浓度。结果,各组织在0.05～6.4μg/g范围内线性关系良好(r0.999),吉他霉素在各组织中回收率均大于65.38%,批内及批间变异系数分别小于5.56%和4.60%,检测限为0.05μg/g。结果表明,此方法重复性好、灵敏度高,可用于猪组织中吉他霉素残留的检测。     

青霉素类抗生素分析研究进展及其应用

青霉素类抗生素具有杀菌活性强、毒副作用小等特点,在临床、动物饲养中应用非常广泛。但使用此类抗生素可能会对某些个体引起过敏反应甚至死亡,同时该类药物的β-内酰胺环不稳定,易受pH值、温度和β-内酰胺酶的影响而开环,原体进入生物体后极易分解,代谢快,检测时限短,因此寻找稳定性好和检测时限长的代谢产物对青霉素类抗生素的检测显得十分重要。本文就近年来青霉素类抗生素的检测方法、稳定性研究、代谢物检测及其在食品中的残留检测、法医学领域中的应用等内容进行了综述。     

碳关税的合法性分析——以WTO为视角

WTO与碳关税有关的规则主要包括GATT1994中的边境调节税、最惠国待遇原则、关税约束原则、国民待遇原则、一般例外条款等。碳关税在本质上是一种与碳排放有关的进口环节边境调节税。GATT1994第20条可能成为碳关税合法的依据。WTO争端解决案例为分析这一问题提供了有益的资料。碳关税的设计只有满足一定的条件,才能符合WTO规则。     

全球气候变化法律应对及“后京都时代”我国的对策

从《联合国气候变化框架公约》到《哥本哈根协议》,国际社会应对气候变化的努力始终未懈,但成效有限。在此过程中,各主要发达国家在国内也做了积极探索,许多方面值得中国借鉴。随着"后京都时代"的即将来临,中国作为世界排放大国必须切实减排。为此,一方面需加强减排国际合作,认真履行国际义务和减排承诺,另一方面应依法合理地推动发达国家履行减排义务,共促减排目标实现,同时对碳关税等新型贸易壁垒可能对我国国际贸易带来的影响和损害也应积极应对。     

胶带粘面上的手印显现

经过比较,证明悬浮液法、碳素墨水法、染色法和物理显影液法对显现胶带粘面上的手印效果较好.     

胶带粘面手印荧光显现剂的研发与应用

目的根据胶带粘面上潜在手印的特点,利用染料和潜手印的理化性质,研究开发胶带粘面手印荧光显现剂。方法在各种胶带粘面上显现潜在手印,并与常规的碳素墨水染色法进行比较。结果在长波紫外线照射下手印呈黄色明亮荧光,手印纹线清晰、连贯,基本不受手印遗留时间、客体表面颜色和性质的影响。结论胶带粘面上的汗潜手印和血潜手印用荧光显现剂显现效果优于碳素墨水染色法,在实际案件的侦破中有较好的应用前景。     

Pacifying uncooperative carbon: examining the materiality of the carbon market

This paper scrutinizes how greenhouse gases are ‘pacified’ so that they can become tradable in the carbon markets. To advance the economization programme and other materialist frameworks, I argue that the existing literature does not pay enough attention to the diverse modes of carbon accounting and, in particular, carbon measurement – the most basic step – is overlooked and undertheorized. Drawing from the ‘critical metrology’ approach, I suggest that we need to take carbon’s diverse materialities seriously in the study of marketization processes. Some carbons are more cooperative than others. I, therefore, argue that it is important to conceptualize ‘pacification’ as a dynamic process that is mediated through materials of varying capacities as well as standards and technologies. The empirical case examined here concerns carbon measurement standards at coal-fired power plants – an ‘extreme case’ in the sense that coal is well-understood and relatively easy to measure. My findings indicate that, even for one of the most ‘cooperative’ carbons, measurement uncertainties are significant and pose challenges for the marketization of carbon emissions. While human actors work to cope with these uncertainties, the contours of the market are ultimately constrained by carbon’s materiality.     

A Small Population Study on Friction Skin Ridges: Differences in Ridge Widths Between Latent and Inked Fingerprints

Morphological changes in the width of latent fingermark ridges occur naturally over time. This could be used to examine the aging process of latents and eventually estimate time of deposition. In a crime context, it is common practice to compare a questioned (aged) fingermark with a database of known (inked) prints. Therefore, in the absence of fresh fingermarks for aging purposes, it is of interest to reveal correlations between these two categories of fingerprints with regard to the widths of their ridges. The present study explores correlations of ridge widths between flat and rolled inked prints with latent fingermarks visualized with carbon black (CB) and titanium dioxide (TiO₂)-based powders among a small population of ten donors. Results revealed consistent differences between the ridge widths of latent and inked prints as well as flat and rolled impressions. Latent fingermarks visualized with CB and TiO₂ powders showed ridges with comparable widths.     

生态文明视域下的环境税收法治省思——从平移路径到并行路径

近年来,环境税收法治的结晶集中体现为环境费改税与环境税立法。要评估环境税收法治在生态文明建设中的表现,庇古税原理是重要标尺之一。由此观之,由于未能严格秉持庇古税原理进行设计,立法者在生态文明建设和政治文明建设任务"竞合"且时间紧迫的情况下,选择了一条成本最小的"平移路径",导致环境税成了迷你版环境税,环境税立法也未能与整个税制的"生态化"统筹起来。对此,应全面、正确理解庇古税理论,统筹环境税法与整个税法制度"生态化"两路并进,不仅从征税范围、税率结构、税收优惠、税权配置等方面完善《环境保护税法》,而且同步优化相关税制,以环境税收法治并行路径来积极推进生态文明建设。     

Detection of Pb,Ba, and Sb in Cadaveric Maggots and Pupae by ICP-MS*

The concentrations of lead (Pb), barium (Ba), and antimony (Sb), characteristic of GSR, were determined in soil sediments and immature (larvae) of cadaveric flies of the family Calliphoridae, by inductively coupled plasma mass spectrometry (ICP-MS). This research refers to a case study from two real crime scenes in which the corpses were in an advanced state of decomposition. In case 1, the victim had holes similar to gunshot wounds, and in case 2, there was no evidence of perforations in the corpse. Soil sediment collection was performed at three different points of the terrain, at a minimum distance of 10 m from the corpse, for cases 1 and 2. In relation to the collection of immatures, larvae were collected in regions of the mouth, nose, and orifices similar to the entry of firearms projectile into the body, for case 1, and collection of larvae and pupae, located on the body and underneath it, for case 2. It was possible to detect and quantify the three elements of interest (Pb, Ba, and Sb) by ICP-MS in both sediment and cadaveric larvae. Concentrations of 4.44, 8.74, and 0.08 μg/g were obtained for Pb, Ba, and Sb, respectively, in the soil for case 1. For the case 2, the concentrations in Pb, Ba, and Sb were from 16.34 to 26.02 μg/g; from 32.64 to 57.97 μg/g and from 0.042 to 0.30 μg/g, respectively. In the larvae, Pb, Ba, and Sb were quantified in cases 1 and 2 with a concentration of 6.28 and 1.78 μg/g for Pb, 1.49 and 2.94 μg/g for Ba, 0.50 μg/g and <LD for Sb, respectively. These new results present the detection of characteristic elements of GSR in cadaveric larvae in humans in a real crime scene, besides highlighting the importance of the study of immature flies, using the ICP-MS technique in forensic analysis.