Sudden Unexpected Death Due to Left Subclavian Artery‐esophageal Fistula Caused by Fish Bone

A 53‐year‐old woman was admitted to the hospital due to unexpected dizziness and died the following morning. To investigate the cause of death, a forensic autopsy along with histological examination was performed 3 days after her death. The major findings of the autopsy were that a fish bone had pierced the left subclavian artery after perforating the esophagus with 680 mL of blood in the stomach and bloody and tarry contents were present in the intestines, and the cause of death was confirmed to be subsequent hemorrhagic shock. Unfortunately, none of her family realized that she had eaten a fish 4 days before the tragedy until the fish bone was found. The present case is rare and instructive. The histopathological findings of left subclavian artery‐esophageal fistula induced by a fish bone can be used as a reference in forensic practice.     

Complex Congenital Heart Malformation Including Transposition of the Great Vessels,Presenting as Sudden Unexplained Infant Death

Transposition of the great vessels (TGV) is a common congenital heart defect that is difficult to diagnose before birth. Antenatal diagnosis is associated with increased survival. Unusual features such as anomalous pulmonary artery origin may delay cyanosis, decreasing clinical suspicion. A three‐week old female infant who had never been cyanotic presented for forensic autopsy due to onset of unresponsiveness at home. History included risk factors for TGV and signs of heart enlargement that were not recognized during life. Cardiac pathology consultation identified D‐type TGV with additional rare anomalies. TGV may present as sudden unexplained infant death (SUID) for forensic autopsy if variant features prevent development of cyanosis. Cardiac pathology consultation is helpful in clarifying these features.     

Repeat Coronary Artery Dissection in Pregnancy: A Case Report and Review of the Literature

Non‐atherosclerotic spontaneous coronary artery dissection (NA‐SCAD) is a rare cause of morbidity and mortality with a propensity for young, healthy, and often peripartum women. NA‐SCAD etiology is poorly understood, with possible hormonal and hereditary mechanisms. Current treatment strategies range from conservative management (often showing resolution on angiographic follow‐up) to invasive angiographic procedures. Rarely, NA‐SCAD has recurred in another coronary artery, ranging hours to years later. We report NA‐SCAD of the right coronary artery (RCA) in a 30‐year old, 3‐month postpartum female with an additional autopsy finding of remote myocardial infarction (MI) in the left anterior descending (LAD) coronary artery territory. The remote MI is consistent with prior NA‐SCAD of the LAD and, given the medical history, may have occurred in the peripartum period of the decedent first pregnancy 3 years earlier. As such, to the best of our knowledge, this may represent the first reported case of NA‐SCAD recurrence in a subsequent pregnancy.     

温病湿热证湿重于热动物模型舌的病理观察

目的：观察温病湿热证湿重于热动物模型舌的病理改变。方法：复制温病湿热证湿重于热动物模型,观察大鼠舌的病理改变,并设湿热组、脾虚组、正常组进行比较。结果：湿重于热组、湿热组固有层乳头密度及核分裂相频数显著增多,与正常组及脾虚组比较差异有显著性;湿重于热组和湿热组固有层均出现炎症、充血及水肿,前者水肿更甚,后者则充血明显。结论：舌的病理改变,可能是湿热证型中舌苔变化的病理基础,并可作为判定湿热轻重的指标之一。     

口蹄疫病毒A/WH/09株结构蛋白P1基因的克隆及其B细胞抗原表位的预测

对口蹄疫病毒(FMDV)A/WH/09株P1结构蛋白基因进行了扩增、克隆及序列测定。结果显示,FMDV A/WH/09株P1基因长2 208bp,包含完整的开放阅读框,编码736个氨基酸,其中VP1长636bp,编码212个氨基酸;VP2长654bp,编码218个氨基酸;VP3长663bp,编码221个氨基酸;VP4长255bp,编码85个氨基酸。采用DNAStar Protean软件对P1蛋白的二级结构、可塑性、亲水性、表面可及性及抗原指数等参数进行了综合分析,并预测其B细胞的抗原表位分布。结果表明,VP1、VP2和VP3上均有多个区域为B细胞抗原优势表位,VP4上也有少量潜在的B细胞抗原表位。与已鉴定的B细胞抗原表位相比,本试验所用方法预测的结果有较高的准确度,是一种较为先进的表位研究方法。     

口蹄疫病毒3C基因与增强型绿色荧光蛋白基因的真核共表达

为验证siRNAs对口蹄疫病毒(FMDV)复制的抑制效果,用反转录聚合酶链反应(RT-PCR)扩增Asia 1型口蹄疫病毒Jiangsu/China/2005株的3C基因,克隆入增强型绿色荧光蛋白(EGFP)表达载体pEGFP-N1中,并进行双酶切、PCR及测序鉴定。将阳性重组质粒转染PK-15细胞,检测EGFP的表达和3C基因转录水平。结果显示,经PCR及双酶切鉴定,目的基因片段大小与预期相符,测序结果与Jiangsu/China/2005株相应序列一致。荧光显微镜和流式细胞仪均检测到细胞内有EGFP表达,实时荧光定量PCR检测到细胞内有3C基因的转录。证实,成功构建了FMDV 3C基因与EGFP共表达质粒并在PK-15细胞中获得了表达。     

牛羊赤羽病病毒环介导等温扩增检测方法的建立

为建立一种能够快速、简便地检测牛、羊赤羽病病毒的分子生物学方法,根据赤羽病病毒特异性S基因的6个特异区域设计了2对引物,首次建立了赤羽病病毒环介导等温扩增(LAMP)检测方法,对LAMP反应条件进行了优化,并将其与普通PCR方法进行比较。结果显示,LAMP检测方法的特异性好,只对赤羽病病毒进行扩增,且操作简便。本研究确定的最佳反应温度为63℃,核酸检测的最低检出限为1.08ng/μL,比普通PCR的灵敏度高2个数量级。该方法为赤羽病病毒的一种最新的快速、简便的检测方法,可用于出入境牛、羊赤羽病的快速检测。     

表达新城疫病毒F基因的重组鸡痘病毒实时荧光定量PCR检测方法的建立

采用荧光染料SYBR Green渗入法,通过对real-time PCR反应条件进行优化,建立了real-timePCR检测方法,用该方法定量分析新城疫病毒(NDV)F基因在重组鸡痘病毒(rFPV-F-VP0)第1、5、10、15、20代次中的表达整合情况。结果表明,建立的标准曲线呈现良好的重复性和特异性,与模板浓度呈现良好的线性关系。在线性浓度范围内,随着模板量的减少,其对应的Ct值相应增大,0.99<相关系数(r2)<1,0.8<扩增效率(E)<1.2,熔解曲线为单一特征峰型。本试验精确定量了外源基因在重组鸡痘病毒中的表达水平,为阐明重组鸡痘病毒的表达机理提供了理论基础。     

负载灭活口蹄疫病毒树突状细胞活化CD8+T细胞的非蛋白酶体依赖途径

为研究树突状细胞加工和呈递灭活口蹄疫病毒(FMDV)抗原并活化CD8+T细胞的途径,用灭活FMDV负载经抑制剂预处理的小鼠单核细胞源树突状细胞(MoDCs),与CD8+T细胞共培养,对照为负载FMDV的正常MoDCs与CD8+T细胞.收集上清液,检测γ干扰素(IFN-γ)的含量.结果显示,试验组CD8+T细胞在共培养的...     

Myocardial hypertrophy induces carotid body hyperplasia

The carotid bodies tend to enlarge after long-standing cardiopulmonary disease. Our objective was to investigate whether cardiac hypertrophy is associated with carotid body hyperplasia. Fifteen autopsy cases with combined left and right ventricular hypertrophy were examined and compared with two control groups (16 cases). The study involved a meticulous dissection of carotid bifurcations, thin serial sections, and morphometric analysis of carotid body volume and cell types (progenitor, dark, light, and sustentacular). There was a significant increase in sustentacular cells in all individuals with cardiac hypertrophy, which was not drug-induced, and accompanied by a similar increase in carotid body volume. Dark or light cell accumulation was detected focally and only in three instances. It appears that the generalized sustentacular cell hyperplasia is the result of long-standing hypoxia, while a superimposed focal prominence of dark or light cells may be proliferative or metaplastic in nature and attributed to short-term hypoxia.