鸭传染性腔上囊病毒VP2基因的克隆与序列分析

应用RT PCR法对分离于当地典型发病鸭群的鸭传染性腔上囊病毒 (IBDV )YL997株进行了VP2基因的克隆与序列分析 ,并和相关毒株进行了比较。结果表明 ,YL997株与STC株的核苷酸和氨基酸同源性只有 92 .5 %和 91.8% ,与超强毒株UK6 6 1的核苷酸和氨基酸同源性均为97.6 % ,而与当地鸡群中分离的超强毒株HN94 2的核苷酸和氨基酸同源性则高达 98.1%和98.4 % ,该毒株的VP2基因序列完全具备了超强毒株的主要特征。     

浅议网络“性息”传播

从犯罪的四个构成要件分析网上传播“性息”行为的渊源和危害,应当认定此类行为构成介绍卖淫罪;对此必须进一步加强研究,尤其是其特殊形态的认定,进而为刑事执法实践提供参考。     

错位与归位:滥用职权罪立法的检讨与重构

对于修订后的刑法所新设的滥用职权罪,目前主流的观点认为其是故意犯罪,但相关的论证实难令人信服。合理的思路或许是区分不同的层面进行解读:从实然的角度分析,现行刑法中确立的滥用职权罪是过失犯罪;但从应然的角度反思,滥用职权罪的正确定位应是故意犯罪。为此,应当通过立法修正的形式对其进行理性重构。     

鸡myostatin基因成熟区段DNA的克隆及其在甲醇酵母中的融合表达

根据GenBank中鸡myostatin(AF019621)成熟区段的cDNA序列设计了1对引物,利用PCR技术扩增了萧山鸡myostatin的成熟区段,构建了重组真核表达载体myostatin-pPIC9K,并在甲醇酵母中融合表达了myostatin蛋白。结果发现,萧山鸡myostatin基因存在2处碱基变异:T204→C204(编码的氨基酸没有变),C205→T205(编码的氨基酸由Pro变成Ser),从而产生了1个EspⅠ或Bpu1102Ⅰ限制性内切酶酶切位点。核苷酸序列同源性分析表明,myostatin基因成熟区段DNA在不同物种间具有高度的保守性。SDS-PAGE鉴定结果表明,表达的目的蛋白(26 ku)具有生物学活性。     

DNA commission of the International Society of Forensic Genetics: Recommendations on the interpretation of mixtures

The DNA commission of the International Society of Forensic Genetics (ISFG) was convened at the 21st congress of the International Society for Forensic Genetics held between 13 and 17 September in the Azores, Portugal. The purpose of the group was to agree on guidelines to encourage best practice that can be universally applied to assist with mixture interpretation. In addition the commission was tasked to provide guidance on low copy number (LCN) reporting. Our discussions have highlighted a significant need for continuing education and research into this area. We have attempted to present a consensus from experts but to be practical we do not claim to have conveyed a clear vision in every respect in this difficult subject. For this reason, we propose to allow a period of time for feedback and reflection by the scientific community. Then the DNA commission will meet again to consider further recommendations.     

谈涉网犯罪——涉网犯罪案件剖析

涉网犯罪在刑事案件发案中所占比例呈上升趋势,利用网络为犯罪提供各种服务的刑事案件越来越普遍.文章结合各种形式的涉网犯罪,对涉网犯罪案件利用网络寻找犯罪对象、准备犯罪工具、联络同伙、实施犯罪、销赃等情况进行仔细剖析,帮助侦查人员了解涉网犯罪,发现犯罪与网络具有的联系,为研究涉网犯罪侦查提供思路.     

片面共犯之成立依据

片面共犯能否成立,在中外刑法理论界一直存在争议。片面共犯应当是成立的:刑法的规定是其成立的法律依据,部分犯罪共同说是其成立的理论基础,司法实践需要是其成立的现实依据。     

The evolution of European legal integration

This article contributes to the development of theories on European integration by testing and exploring statistical models on the long-term development of legislative activity of the European Commission. Drawing on legal information gained from the European Union’s PreLex database and analyzing it with the help of statistical analyses, we map out growth patterns of EU law between 1976 and 2003. We construct time-series models and models based on non-linear regression. While the performance of models based on the traditional theoretical approaches, intergovernmentalism and neo-functionalism, is rather poor, the analysis suggests that nonlinear dynamic models might be an interesting avenue for future conceptualizations of the EU integration process. This article is based on a paper presented at the ECPR Standing Group on the European Union Second Pan-European Conference on EU Politics, “Implications of a Wider Europe: Politics, Institutions and Diversity”, 24–26 June 2004, Bologna, Italy. We would like to thank the seminar participants for useful comments.     

诉讼欺诈行为研究——以刑法为视角的分析

诉讼欺诈是一种独特的危害社会的行为。我国刑法理论和司法实践对诉讼欺诈行为的定性分歧较大。事实上,诉讼欺诈完全符合诈骗罪的本质特征,因而应按诈骗罪定罪量刑。诉讼欺诈行为侵犯双重具体的社会关系,但其直接客体是单一客体。诈骗罪中的被骗人与被害人不必是同一人,但被骗人与财产处分者必须是同一人;在被骗人与被害人不一致的情况下,应当以被骗人为基准来判断处分意思和处分行为。     

犯罪国际化的动因及特点

全球化背景下世界的开放性为犯罪的国际化提供了方便，各国法律、政策的差异性刺激了犯罪的国际化，现代技术的广泛应用为犯罪的国际化提供了技术支持．全球化引起的“失范状态”加剧了犯罪国际化的倾向。全球化形势下犯罪国际化是以计算机网络信息技术为其主要手段、以有组织犯罪为其主要形态、以毒品犯罪和洗钱犯罪为其典型犯罪类型并以黑社会性质的犯罪为其主要发展趋势的。