HPLC-MS/MS法快速检测人体血液中的夹竹桃毒素

目的采用高效液相色谱-串联质谱法(HPLC-MS/MS)检测人体血液中夹竹桃苷、夹竹桃苷乙。方法采用乙腈沉淀蛋白法处理血液,HPLC-MS/MS法检测,采用MRM记录方式,保留时间和定性离子对定性,标准曲线法定量。结果夹竹桃苷、夹竹桃苷乙的检测限均在0.5ng/m L,线性范围在1ng/m L~1mg/m L,回收率为75.2%~95.7%。结论本方法操作简便,灵敏度高,可应用于中毒案件中人体血液中两种夹竹桃毒素(夹竹桃苷和夹竹桃苷乙)的快速检测。     

20723例亲子鉴定中19个STR基因座的突变分析

目的观察并分析肯定亲权关系的案件,探索STR基因座的突变规律。方法采用Goldeneye 20A试剂盒对20723例肯定亲权关系的案件筛选等位基因突变事件,统计各基因座的突变率和突变等位基因的来源、片段大小、突变步数及重复单位的增加或减少情况,分析突变相关因素的特点。结果 19个STR基因座共发现548例突变,观察到557个突变事件,基因座的突变率为0.07‰~2.23‰。父系突变与母系突变的比例为3.06∶1。突变以一步突变为主,增加与减少重复单位的情况相当;二步以上(含二步)突变更易出现重复单位减少。突变主要发生于中等位基因,重复单位增减比例相当,长等位基因突变中重复单位减少显著多于增加。父系突变出现重复单位增加与减少的比例相当,母系突变重复单位减少较增加多见。结论各基因座的突变率差异具有统计学意义,当出现1~2个基因座不符合遗传规律时,应当加测其他检测系统,并结合突变基因座的信息计算PI值,以进一步明确鉴定意见。     

Examining the Impact of Early Childhood School Investments on Neighborhood Crime

This study tested the hypothesis that investments in early childhood schools have short-term crime reducing effects in neighborhoods. Time series data from the city of Tulsa, Oklahoma, were analyzed to evaluate the effects of an early childhood school built in the neighborhood of Kendall-Whittier as part of a larger neighborhood revitalization plan, on violent and property crime. Results revealed that after controlling for city-wide crime trends and monthly fluctuations, violent crime declined significantly in Kendall-Whittier. Further analysis indicated that the possible crime-reducing effects of school investments on violent crime spread beyond Kendall-Whittier, and no displacement was found. The results for property crime were mixed. The study demonstrates the use of clustering analysis, a useful tool in neighborhood-level research to identify comparison neighborhoods. The findings shed light on the possibility that investments in early childhood schools can yield results in a shorter term than anticipated, making them a desirable component of urban revitalization.     

Predictors of Initial Court Agreement and 1‐Year Relitigation in Title IV‐D Contested Paternity Cases

We examined potential predictors of initial court agreement and 1‐year relitigation in a sample of contested paternity cases involving unmarried parents coming to court to establish paternity, child support, and other issues. Cases participated in an RCT of a parent program and of a waiting period between establishment of paternity and court hearing. We controlled for RCT study factors and used baseline assessment data to predict likelihood of reaching full agreement in the initial court hearing and relitigation in the following year. Findings suggest that cases in which parents get along better outside of court are more likely to reach agreement and less likely to return to court. Additionally, particular parent demographics predict lower likelihood of reaching initial agreement (e.g., parents are non‐White, father earns below $10,000 yearly), more relitigation (e.g., parents are non‐White, mother earns above $10,000 yearly, father has children with others), and less relitigation (e.g., father earns above $10,000 yearly). Child demographics and most parent relationship characteristics did not predict outcomes. We discuss findings and offer suggestions for court interventions.     

Adaptive and Maladaptive Gatekeeping Behaviors and Attitudes: Implications for Child Outcomes After Separation and Divorce

Gatekeeping has been used as a theory and a measure to describe and assess family dynamics within the context of separation and divorce. In this article, we explore adaptive and maladaptive gatekeeping behaviors and attitudes that can affect the other parent's relationship with the child. Implications are presented for connecting adaptive and maladaptive gatekeeping responses to child outcomes of safety, well‐being, and positive parent–child relationships following separation and divorce. We build on the recent attention to gatekeeping as a potential framework within the child custody context.     

Scanning memory with Yara

Memory analysis has been successfully utilized to detect malware in many high profile cases. The use of signature scanning to detect malicious tools is becoming an effective triaging and first response technique. In particular, the Yara library and scanner has emerged as the defacto standard in malware signature scanning for files, and there are many open source repositories of yara rules. Previous attempts to incorporate yara scanning in memory analysis yielded mixed results. This paper examines the differences between applying Yara signatures on files and in memory and how yara signatures can be developed to effectively search for malware in memory. For the first time we document a technique to identify the process owner of a physical page using the Windows PFN database. We use this to develop a context aware Yara scanning engine which can scan all processes simultaneously using a single pass over the physical image.     

大熊猫轮状病毒CH-1株外衣壳蛋白VP4基因的克隆与生物信息学分析

利用MA-104细胞培养增殖大熊猫轮状病毒CH-1株,提取总RNA,运用RT-PCR扩增外衣壳蛋白VP4基因,将其与pMD19-T simple vector连接并转化DH5α,经PCR扩增和测序分析进行鉴定,用生物信息学软件预测其功能,并构建系统进化树。结果显示,成功获得大熊猫轮状病毒VP4蛋白基因,长2 362bp,包含一个2 331bp的开放阅读框,编码776个氨基酸,测序结果在GenBank中的登录号为HQ641296。生物信息学分析表明,VP4蛋白基因编码产物分子质量为86 768.8u,理论等电点为5.56,半衰期为30h(体外哺乳类网状细胞),不稳定系数为28.64,脂肪指数为82.53,总平均亲水性为-0.265,最大疏水性为2.244,最小疏水性为-2.911;无信号肽序列;跨膜结构分析显示VP4蛋白无跨膜区,位于病毒膜外区;在VP4序列中的抗原决定簇主要位于VP4的N-末端和C-末端。预测其可能包含8个N-糖基化作用位点,15个蛋白激酶C磷酸化作用位点、15个酪蛋白激酶Ⅱ磷酸化作用位点、12个N-豆蔻酰化位点。系统进化分析显示,大熊猫轮状病毒的VP4基因与猪轮状病毒的VP4基因的进化距离最...