复合扩增检测D9S302、D18S865和PLA2A基因座多态性

应用 PCR复合扩增技术、变性聚丙烯酰胺凝胶电泳和银染法显色检测技术,对云南省汉族人群 100名个体的 D9S302、 D18S865和 PLA2A基因座进行了研究,分别检出 10、 7和 7个等位基因,基因型频率分布均符合 Hardy－ Weinberg平衡,家系调查证实三基因座的传递遵循孟德尔遗传规律。三基因座累积个人识别率为 0.999 7,累积非父排除率为 0.992 9。证实了复合扩增 D9S302、 D18S865和 PLA2A三基因座是法医学个人识别和亲权鉴定中极有价值的检测方法。     

广西壮、汉族人群F13A01、FESTPS、vWA三个STR基因座的遗传多态性

采用复合扩增、PAG电泳分离和银染检测技术,对广西壮、汉族人群的F13A01、FESFPS、vWA等3个STR基因座进行遗传多态性研究.3个基因座的基因型在广西壮、汉族人群的频率分布均符合Hardy-wein-berg平衡.3个STR基因座在广西壮族人群的个体识别力(DP)分别为0.8205、0.8566和0.9239;在广西汉族人群的个体识别力(DP)分别为0.8256、0.8455、0.9191.     

桃红四物汤中5种主要成分在脑缺血再灌注模型大鼠体内组织分布的研究

目的 研究脑缺血再灌注模型大鼠在灌胃桃红四物汤(Taohong Siwu Decoction, THSWD)后,5种主要活性成分(藁本内酯、苦杏仁苷、芍药苷、羟基红花黄色素A、阿魏酸)在体内组织分布的差异和规律。方法 采用线栓法构建大鼠大脑中动脉闭塞(middle cerebral artery occlusion, MCAO)模型,然后再灌注24 h建立脑缺血再灌注大鼠模型;将模型复制成功的大鼠灌胃THSWD(50 g/kg)后,于不同时间点采集组织(心、肝、脾、肺、肾、脑),样品处理后采用UPLC-MS技术检测各组织中待测成分的含量。结果 各成分在模型大鼠各组织中分布广泛,在肝、肾组织中浓度较高,其中藁本内酯在脑组织中有较高浓度;苦杏仁苷在肺部具有组织分布特异性;芍药苷、羟基红花黄色素A在大鼠肝、脾、肺组织具有较高分布比例,阿魏酸在脑组织中未检出。结论 THSWD中5种主要活性成分在各组织中分布广泛,各组织不同时间点的分布趋势也存在较大差异。     

美国中小学校园枪击案管窥

本文例举了美国中小学近年来发生的枪击案,分析其特点、背景,指出其危害,引发对我国中小学教育的思考。     

HLA—A基因座分步PCR—SSP分型研究

目的使HLA基因分型能应用于法医常见检材的个人识别。方法 建立检测HLA—A基因座的分步PCR—SSP方法。先用一对HLA—A基因座特异的引物作第一次扩增,以所得产物为模板,分别用对HLA—A30、A31、A33特异的3对引物作第二次扩增,二次扩增的产物经电泳判型。结果 1130例血清分型为HLA—A30、A31、A33的血痕,其PCR—SSP分型和血清分型的不符合率为29％;室温保存2年的精斑、唾液斑,保存18年的血痕第一次扩增均获得满意的结果。结论法医亲子鉴定和个人识别宜用基因分型替代血清分型。HLA—A基因座分步PCR—SSP基因分型适用于法医检材。     

试论学习型机关的建立

在新的历史条件下 ,创建学习型的机关 ,要树立终身学习的理念 ;精心培育机关文化 ;建立机关公务员培训制度、激励约束制度 ,确保干部培训制度化、经常化、规范化 ;不断自我超越 ,实现共同目标。     

Reading the spirits in Julia Alvarez’s A Wedding in Haiti

Dominican writer Julia Alvarez’s 2012 memoir, A Wedding in Haiti: The Story of a Friendship, documents her first-time travel from the Dominican Republic to Haiti over land in order to attend the wedding of Piti, a young Haitian man who had worked for her and her husband for a number of years. She deploys the literary non-fiction genre of memoir, a genre that is relatively new to her, to explore both the Dominican (collective) relationship with Haiti and her individual relationship with both Haiti and its people centering on her connection to Piti and his family. Drawing on Vodou, a nature religion that strives for balance, this article explores Alvarez’s memoir through several lwa. I argue that the lwa are deployed and manifest as mechanisms from within Vodou epistemology to restore balance to a world relationship between Haiti and the DR that reverberates in the metaphysical world. Examinations of this unequal relationship between the two nations are even more prescient contemporarily considering the recent decision by the Dominican Constitutional Court to strip children of “irregular” migrants born in the DR after 1929 of their Dominican citizenship.     

A Thorny Issue

正Recurring credit crunches in the banking system has exposed problems that require institutional reform China’s banking system suffered another credit crunch at the end of 2013.On December 19,trading in the high-value payment system was extended by an extra half an hour,followed by rumors of breaches of contract among banks.Although relevant departments clarified the rumors,interbank     

A Weaker Union

正True love,freedom of choice and even wealth drive recent surge in divorce rate Wu Minqiang,70,has devoted a significant part of his life to providing free marriage counseling for couples considering a divorce.     

What Mr. Spock told the earthlings: the aims of political philosophy,action-guidingness and fact-dependency

Abstract

Recently, G. A. Cohen introduced an influential distinction between fact-sensitive and fact-insensitive principles arguing that all basic normative principles are of the latter type. David Miller rejects this claim submitting that the validity of basic normative political principles depends on some general propositions about human nature and societies; for example, that men’s generosity is ‘confined’ and that nature has made ‘scanty provision’ for his wants. Miller ties this view of the nature of basic political principles to the view that political philosophy ought to guide people engaged in real-world politics and claims plausibly that in order to fulfil this purpose, political philosophy must be informed by social science. I argue that Miller neither succeeds in showing that basic principles can be fact-sensitive, nor establishes any connection between the Cohen-Miller disagreement on fact-sensitivity, on the one hand, and the nature and aims of political philosophy, on the other hand.