D3S1358等7个DNA位点的荧光法在法医学中的应用

报道了D3S1358、D16S539等7个DNA位点的复合扩增和四色荧光分析技术。经310自动测序仪检测7个位点 ,实现了STR位点同步扩增和自动检测目的。同一性实验表明 ,同一个体肌肉、血液、唾液和头发等组织STR位点基因型完全一致。对2个四代家系、3个三代家系的调查结果表明 ,这些位点符合孟德尔遗传定律。D3S1358、D16S539等7个位点对陈旧、微量的检材适用性很强,灵敏度达75pg ,在法医个人识别和亲子鉴定中有着重要意义     

DNA Analysis and Document Examination: The Impact of Each Technique on Respective Analyses

Threatening letters, counterfeit documents, and anonymous notes can commonly be encountered in criminal situations. Such handwritten documents may encourage DNA to transfer from the writer's hands and lower arms when these areas come into contact with the document. As any DNA transferred is likely to be at a low level, sensitive low copy number (LCN) DNA analysis can be employed for testing document exhibits. In this study, we determine locations on the document that are most commonly touched during writing and handling and compare DNA recovery from these sites. We describe the impact of DNA sampling on subsequent document examination techniques including the ESDA^® and likewise the effect of the ESDA^® and two other document examination techniques on subsequent DNA analysis. The findings from this study suggest that DNA results can be obtained through targeted sampling of document evidence, but that care is required when ordering these examination strategies.     

Determination of Nitrite in Whole Blood by High‐Performance Liquid Chromatography with Electrochemical Detection and a Case of Nitrite Poisoning

Although nitrite is widely used in meat processing, it is a major toxicity hazard to children and is responsible for the blue‐baby syndrome. A simple and effective method to determine nitrite in whole blood has been devised using ion chromatography with suppressed conductivity detection. The blood sample was deproteinized by adding acetonitrile and purified with mini‐cartridges to remove hydrophobic compounds, chloride ions, and metal ions. An aliquot of the filtrate was injected onto the ion chromatography. The retention time for nitrite was 13.8 min and the detection limit of nitrite in whole blood was 0.4 μmol/L. The calibration curve was linear (r² = 0.9999) over the concentration working range. The blood nitrite concentration of a victim who attempted suicide by ingesting sodium nitrite powder was determined using the present method. The basal levels for nitrite in human blood was determined with 7.1 ± 0.9 μmol/L (n = 12).     

Blind Detection of Region Duplication Forgery Using Fractal Coding and Feature Matching

Digital image forgery detection is important because of its wide use in applications such as medical diagnosis, legal investigations, and entertainment. Copy–move forgery is one of the famous techniques, which is used in region duplication. Many of the existing copy–move detection algorithms cannot effectively blind detect duplicated regions that are made by powerful image manipulation software like Photoshop. In this study, a new method is proposed for blind detecting manipulations in digital images based on modified fractal coding and feature vector matching. The proposed method not only detects typical copy–move forgery, but also finds multiple copied forgery regions for images that are subjected to rotation, scaling, reflection, and a mixture of these postprocessing operations. The proposed method is robust against tampered images undergoing attacks such as Gaussian blurring, contrast scaling, and brightness adjustment. The experimental results demonstrated the validity and efficiency of the method.     

Determination of Inorganic Ion Profiles of Illicit Drugs by Capillary Electrophoresis

A portable capillary electrophoresis instrument with dual capacitively coupled contactless conductivity detection (C⁴D) was used to determine the inorganic ionic profiles of three pharmaceutical samples and precursors of two illicit drugs (contemporary samples of methylone and para‐methoxymethamphetamine). The LODs ranged from 0.10 μmol/L to 1.25 μmol/L for the 10 selected cations, and from 0.13 μmol/L to 1.03 μmol/L for the eight selected anions. All separations were performed in less than 6 min with migration times and peak area RSD values ranging from 2 to 7%. The results demonstrate the potential of the analysis of inorganic ionic species to aid in the identification and/or differentiation of unknown tablets, and real samples found in illicit drug manufacture scenarios. From the resulting ionic fingerprint, the unknown tablets and samples can be further classified.     

基于深度学习的人体肋骨骨折智能检测技术

目的将人工智能中的深度学习技术应用到人体肋骨骨折识别,实现人体肋骨骨折智能检测,提高法医肋骨骨折诊断效率。方法采集3143例人体胸部X线数字影像(2602例用于训练,541例用于测试),标注肋骨骨折特征点,通过多层网络堆叠,分层、分级主动学习原始数据高度抽象的特征表述,并将此特征反馈至检测器进行骨折检测,输出骨折位置及相应置信度。结果基于深度学习的人体肋骨骨折检测准确率在90%以上。结论基于深度学习的人体肋骨骨折检测准确率较高,可用于辅助法医进行肋骨骨折识别诊断、检验鉴定等,本研究可为人体其他部位骨骼损伤智能检测提供参考。     

对预审若干问题的思考

侦审合一在我国推行十余年来虽取得一些成绩,积累了一些经验,但现实中仍然暴露出不少问题。在当前预审业务相关机构多元化格局已经形成的形势下,既要从长远的角度展望我国预审制度的未来发展方向,又要立足现实对我国现阶段的预审进行理性定性,以寻求预审理论研究与实践结合的契合点,促进预审程序在刑事诉讼中发挥应有的作用,进而推进我国预审制度的良性发展。     

论票据抗辩对票据犯罪侦查的作用

票据抗辩是票据债务人的自我保护方式,相对于票据债权人的请求权而言,也是票据债务人的一项权利。票据抗辩的事由包括基于票据本身的事由发生的抗辩,也称物的抗辩;基于人的事由发生的抗辩,也称人的抗辩。票据制度在规定抗辩的同时也对抗辩进行了限制,在限制的同时又规定了例外。这样一个完美的制度对票据犯罪侦查具有抑制票据犯罪的得逞、为票据犯罪侦查赢得时间和确定犯罪嫌疑人等方面的作用。     

经侦职能视角下的经侦“战学研”教学模式探讨

经济犯罪侦查工作所面临的新形势和新困难对经侦队伍的建设提出"打击、服务、参谋"三大职能要求,这也是对经济犯罪侦查后备人才培养的经侦教育教学提出的新课题。将经侦三大职能融入经侦专业人才培养"战学研"模式需要从三个方面进行思考:一是"打击与战"的融合,即课程体系方面紧跟实战,教学内容贴近实战,教学方法融入实战;二是"服务与学"的交流,即加强实践教学体系的建立与实践能力的培养;三是"参谋与研"的互动,即科研转化教学与创新精神的孕育。     

Application of mist to fingermark detection: Misting with high-boiling-point liquid containing p-dimethylaminocinnamaldehyde and cyanoacrylate

In order to detect latent fingerprints that could be damaged by liquid or powder reagents, non-destructive processes such as gaseous reagents have been developed. In this report, we propose the use of fine mist generated when hot vapor of high-boiling-point liquids is rapidly cooled by surrounding air for fingermark detection. Octyl acetate (OA), 2-phenoxyethanol (2PE), and methyl decanoate (MD) were found to efficiently produce mist when heated to 230°C. By combining these liquids with p-dimethylaminocinnamaldehyde (DMAC) and cyanoacrylate (CN), our team demonstrated effective fluorescence staining of cyano-treated fingermarks using DMAC/OA misting or DMAC/2PE misting, and one-step fluorescence detection of latent fingermarks without cyanoacrylate treatment using DMAC/OA/CN misting or DMAC/MD/CN misting. Fingermark fluorescence was efficiently observed by excitation with a blue LED light (max. wavelength 470 nm) equipped with an interference filter and passing through a 520 nm long-pass filter. We successfully obtained fluorescent images from fingermarks on several substrate materials using the developed misting method.