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1.
迟发性外伤性脑内血肿脑组织GFAP、S-100和NSE的免疫组织化学研究 总被引:1,自引:1,他引:1
应用免疫组织化学ABC法和S-P法,对6例伤后1.5天至2年发生的迟发性外伤性脑内血肿(DTICH)脑组织标本进行神经胶质细胞内胶质纤维酸性蛋白(GFAP)、S-100蛋白(S-100)和神经元细胞内特异性烯醇化酶(NSE)变化和应用价值的研究。尸检和组织学检查发现有脑挫伤等病变。6例DTICH脑组织挫伤及周围区均有明显的GFAP、S-100阳性的细胞变化,细胞数目增多,胞体肥大、增生,免疫组化反应产物增多、深染;神经元细胞NSE免疫组化染色均呈阴性改变。结果表明:6例DTICH脑组织中GFAP、S-100的NSE的免疫组织化学变化是有一定时间间隔的外伤性陈旧性改变,在DTICH的病理诊断上具有重要价值,可作为DTICH法医学鉴定的一个辅助手段。 相似文献
2.
目的 观察不同浓度新藤黄酸(gambogenic acid,GNA)对脑胶质瘤细胞U87增殖及自噬的影响。方法 倒置显微镜下观察细胞形态变化,采用噻唑蓝法检测细胞的存活率,单丹磺酰尸胺(monodansylcadaverine,MDC)染色法检测自噬泡的形成,吖啶橙(acridine orange,AO)染色流式细胞仪观察酸性囊泡细胞器的数量变化,Westernblot法检测自噬相关蛋白微管相关蛋白1轻链3(microtubule associated protein 1 light chain 3,LC3)- Ⅱ/LC3- Ⅰ以及Beclin- 1的表达变化。结果 GNA在1~32 μmol/L浓度范围抑制U87细胞的增殖;MDC染色表明GNA促进U87细胞内自噬泡的形成;AO染色表明GNA增加U87细胞内酸性囊泡细胞器的数量;Western blot结果显示,GNA作用U87细胞后,LC3- Ⅱ/LC3- Ⅰ比值增大,Beclin- 1的表达增加,提示细胞内自噬活性增强。结论 GNA在一定剂量和时间范围内抑制脑胶质瘤细胞U87细胞的增殖可能与诱导U87细胞自噬的发生有关。 相似文献
3.
Abstract: A new screening method for detecting gamma-hydroxybutyric acid (GHB) in drink matrices, using the IonSense, Inc. (Saugus, MA) direct analysis in real time (DART) ion source coupled to a JEOL exact mass time-of-flight mass spectrometer (AccuTOF), was validated and compared with the current screening methodology. The DART ion source allows for analysis of samples under ambient conditions with little to no sample preparation. Fifty drink specimens were spiked at levels of 1, 2, 3, and 4 mg/mL GHB, and analyzed on the AccuTOF-DART. Positive detection of GHB occurred for each of the samples at each concentration level, giving 100% accuracy for the samples tested. Twenty-five of the 50 drink specimens were spiked at 1 mg/mL GHB and tested using a color test known as the GHB Color Test #3. Only two of these 25 specimens tested positive for the presence of GHB, giving only 8% accuracy. Implementation of this new methodology as a screening tool for GHB analysis will quickly eliminate negative specimens allowing the examiner to focus analysis time on those that screened positive. 相似文献
4.
5.
尿液、血液中γ-羟丁酸的气质联用法分析 总被引:3,自引:0,他引:3
目的为尿液、血液中γ-羟丁酸(gamma-hydroxybutyricacid,GHB),γ-羟丁酸内酯(gamma-butyrolactone,GBL)和1,4-丁二醇(1,4-butanediol,1,4-BD)的鉴定提供方法和依据。方法100μl尿液或血液以GHBd6为内标,经乙酸乙酯提取、BSTFA衍生化后,用GC/MS法分析。结果测尿液中内源性GHB的线性范围是20-800ng/ml,R2=0.9995,最低检出限为10ng/ml(S/N≥3);测尿液、血液中外源性GHB的线性范围为5-60μg/ml,R2分别为0.9999和0.9928。相对回收率为99%-104%。以所建方法测定了健康志愿者尿液中内源性GHB含量,并考察了健康受试者外源性GHB的代谢情况。结论所建方法准确、便捷、省时、选择性好,适用于法医毒物学鉴定。 相似文献
6.
目的建立尿中2,4-D、2,4-DP、MCPA、MCPP等4种苯氧羧酸类除草剂的分析方法;方法液液提取分离,乙醚为提取液,DCPA为内标,硫酸正丁醇酯化衍生化,气质联用分析法分析;结果尿中4种除草剂添加样品的相对回收率在80%以上,检测限都在5ng/ml以下,对中毒兔尿样进行了分析;结论对实际发生的中毒案件分析有足够的灵敏度。 相似文献
7.
Juliann Emmons Allison 《International Environmental Agreements: Politics, Law and Economics》2005,5(4):405-414
Deregulation and the combined threats of energy crises and global warming concern nations around the world, yet these issues
continue to be addressed more directly by domestic regulatory systems than by international institutions. The present analyses
of the integration of distributed sources of power generation (DG) into California’s electric utility system suggests that
domestic environmental dilemmas with international repercussions provide an obvious entrée for global environmental policy
specialists into the practice of environmental policy-making and law. Here I review current scholarship on policy networks
that illuminates the contributions that technical and policy experts can make to such networks surrounding environmental issues.
I then introduce the key members of California’s “clean DG” policy network that emphasizes the role of academic experts in
this influential political system, and discuss how my own research has impacted the development of the state’s DG policy.
I conclude that scholars are well positioned to observe and engage domestic and international environmental policy networks,
and thereby also to influence environmental politics and law. 相似文献
8.
Diagnostic Value of PSA and AP Tests for the Detection of Spermatozoa in Postmortem Swabs from the Genital and Anal Region in Males 下载免费PDF全文
Laurence Weitzig M.D. Ann Sophie Schroeder M.D. Ph.D. Christa Augustin Ph.D. Tobias Raupach M.D. M.M.E. Susanne Sehner M.Sc. Sven Anders M.D. M.M.E. 《Journal of forensic sciences》2015,60(1):41-44
The aim of this study was to clarify whether positive results for prostate‐specific antigen (PSA) and acid phosphatase (AP) occur in postmortem swabs from the genito‐anal region in males (n = 80; 4 regions) and females (n = 20; 3 regions) and to calculate the positive predictive value (PPV) concerning the presence of spermatozoa. In male subjects, the highest incidence of positive test results was found in urethral swabs (PSA 76%, AP 71%) and the lowest frequencies appeared in perianal and rectal swabs (15–20%). Microscopic evaluation for spermatozoa was positive between 39% in urethral swabs and 1% in rectal swabs. PPV regarding positive identification of spermatozoa was 33.3% for PSA and 31.5% for AP. The combination of both tests yielded a PPV of 38.2%. In female cases, no spermatozoa were identified, and one case was PSA‐ and AP‐positive in perianal swabs. Our findings indicate that PSA and AP tests are of limited value for the postmortem detection of spermatozoa in male subjects. 相似文献
9.
Roger W. Byard M.D. 《Journal of forensic sciences》2015,60(3):812-815
The ingestion of corrosive substances may produce severe burns to the upper aerodigestive tract and stomach, particularly if the pH is greater than 12 or less than two. There is a biphasic age grouping with adult cases most often involving self‐harm and pediatric cases accidental ingestion. Three cases are reported to demonstrate characteristic features following the ingestion of potassium hydroxide, glacial acetic acid and Lysol®, respectively. All deaths were due to the effects of caustic burns to the upper aerodigestive tract, esophagus and stomach with perforation and/or hemorrhage. The extent of injuries in these cases depends on the nature, amount, and concentration of the agent and on the exposure time. A point to note at autopsy is that tissue damage may also occur from postmortem exposure. Typical injuries involve perioral, limb, and trunk burns, with extensive aerodigestive liquefactive/coagulative necrosis causing hemorrhage and perforation. 相似文献
10.
有机磷农药薄层色谱溴酚蓝显色法的改进 总被引:1,自引:0,他引:1
目的对有机磷农药薄层色谱溴酚蓝显色法进行改进,使该检验方法更完善。方法采用薄层色谱溴酚蓝—柠檬酸显色。结果显色背景呈纯正的浅黄色,并且颜色稳定。结论该方法操作简单、快速、检测灵敏度高。 相似文献