6FAM-HEX-TMR-ROX四色荧光分析体系的构建

目的为在310基因分析仪上进行6FAM-HEX-TMR-ROX四色荧光STR复合扩增分型建立基础条件。方法以pGEM载体作为靶DNA,设计引物扩增获得500~80bp的13个长度不等DNA片段,以这些片段纯化物作为模板DNA,分别扩增获得6FAM、HEX、TMR和ROX标记的M atrix标准物,用4种M atrix标准物在310基因分析仪上构建可用于6FAM-HEX-TMR-ROX四色荧光分析的M atrix文件。结果扩增所得的13个非标记片段,长度分别为80、100、120、140、160、180、200、220、260、300、340、400、500bp,在非变性聚丙烯酰胺凝胶连续电泳-银染凝胶上均表现为单条带,6FAM、HEX、TMR、ROX分别标记的片段通过310基因分析仪检测均为单峰。混合ROX标记的80、120、180、220、260、300bp的6个片段获得的内标,显示出良好的线性关系。结论建立了有效的6FAM-HEX-TMR-ROX四色荧光分析M atrix,为进行多个STR基因座荧光标记复合扩增检测奠定了基础。     

论行政证据与行政诉讼证据的相异及衔接

行政证据与行政诉讼证据既相区别又相联系 ,并且存在较多衔接关系 ,从理论和实践上都有正确识别之必要。它们的相异主要表现在 :性质、目的、范围、证明对象、调查取证的阶段等方面。二者的衔接主要表现在 :行政诉讼的一部分证据来源于行政证据 ,二者都具有证据的基本属性、种类划分基本相同。区分行政证据和行政诉讼证据 ,不仅适应了法治行政的潮流 ,而且有助于树立现代法治和正当程序观念 ,促进行政程序价值的实现     

利用现代物证分析技术推断毒品的来源

利用现代物证分析技术推断毒品的来源,是侦查毒品案件的客观要求,对打击毒品犯罪具有重大意义。随着气相色谱同 位素比值质谱、电感耦合等离子体质谱等现代分析技术的出现,使得我们利用现代物证分析技术推断毒品来源成为可能。     

The effectiveness of forensic evidence in the investigation of volume crime scenes

This study investigates the effectiveness of forensic evidence in UK volume crime investigations. The main aim was to identify characteristics of forensic evidence that influence its effectiveness in converting detections into criminal charges, as well as to critically consider the effectiveness of a recent service level agreement (SLA) implemented by Wiltshire Police, which aimed at reducing CSI attendance. The sample consisted of 445 police recorded cases received from Wiltshire Police. Presence or absence and location-related characteristics of fingerprint, DNA, and footwear evidence were evaluated on the effectiveness of forensic evidence and examined within the contexts of different volume crimes. Results showed a high level of correlation in converting detections into criminal charges where the presence of DNA, footwear, and multiple evidence types was recorded; and a positive correlation between forensic evidence ineffectiveness and presence of fingerprints, particularly in residential burglaries. Differences between individual offence types were expressed. The most prominent feature influencing the effectiveness of forensic evidence was found to be related to the movability of the exhibit associated with the recovered evidence, with DNA recovered from non-movable items presenting the strongest effectiveness. Cases processed after the implementation of the SLA did not show significant differences in forensic evidence effectiveness as compared to cases processed prior to the SLA, however, they demonstrated a lack in effectiveness of DNA evidence. The findings of the current research provide a better understanding of the contextual influences on the potential of forensic evidence and can support improvement of crime scene screening and CSI resource deployment.     

医疗损害鉴定意见存在问题与对策

鉴定意见作为专门性问题的鉴定结果,是诉讼案件得以正确处理的重要依据,其对诉讼结果的影响不言自明。鉴定意见不仅关乎证据制度,也涉及司法体系和诉讼程序。医疗领域的诉讼因裁判者囿于知识的局限,鉴定意见的作用更为突出。长期以来,鉴定问题已严重影响到司法裁判的效率与公正。本文通过梳理鉴定意见在医疗损害诉讼各环节中存在的问题,剖析鉴定方式、鉴定主体、文书内容以及审查采信方面等问题的成因,提出确立以自然人为中心的“鉴定人——专家辅助人”的“双翼”医疗损害鉴定人制度,规范鉴定意见内容和表述方式,完善质证程序等措施。     

Evaluation of a Visualization Assay for Blood on Forensic Evidence

In forensics, bloodstains on dark fabrics might be invisible for the naked eye. Although several visualization, presumptive, and confirmatory blood tests have been developed, all have one or more disadvantages, especially on DNA analysis. We report here the use of a visualization assay that can visually detect blood drops up to 1/20 dilution. In this assay, the fabric is placed between two wet filter papers and covered by glass surfaces on both sides. Pressure is applied on the glass surfaces in which bloodstains transfer onto the filter papers through capillary forces. Detected stains can be tested with other more sensitive presumptive blood tests performed on the filter paper. Even more, DNA analysis can be performed on the transferred bloodstains. The presented visualization assay is easy to perform, extremely cheap, requires little hands on time, and does not affect bloodstain pattern analysis.     

A Rapid and Efficient Method for Evaluation of Suspect Testimony: Palynological Scanning

A rapid method for evaluating suspect testimony is valuable at any stage in an inquiry and can result in a change of direction in an investigation. Rape cases, in particular, can present problems where a defendant renders DNA analysis redundant by claiming that the claimant consented to have sexual relations. Forensic palynology is valuable in confirming or eliminating locations as being crime scenes, thus checking the testimony of both parties. In contrast to some forensic disciplines, forensic palynology can provide critical information without time-consuming full analysis. Two cases are described where the palynological assemblages from comparator samples of pertinent places were compared with those obtained from clothing of claimants and defendants. The results of rapid microscopical scanning of relevant preparations led to early confessions, thus obviating the need for costly analyses and protracted court proceedings. A third case demonstrates the unbiased nature of this technique where a man, although innocent of any offense, lied about having visited the crime scene for fear of prosecution. This highlights the need for sensitive policing in claims of rape.     

“直接证据”的迷思

将直接证据与间接证据等区别开来,强调前者的重要性和优越性,是一种流布甚久的普遍观念。能否进行这种区分、如何区分以及这种区分是否具有规范意义等问题,在近年来也受到了理论研究的关注。从诉讼证明的认知构造来看,"直接证据"的界说应当区分严格的理解和日常习惯的理解;无论哪一种界说,都没有多少实际的规范意义。比较而言,关于直接证据的诸种传统界说多是有问题的;近年来的论争,无论是何种论说,都未能促进人们更好地理解此方面的问题。应当放弃在日常习惯理解意义上界分直接证据与间接证据等的视角来制定相关证据规则的努力,注重对前者的证明力进行审查。     

How Do We Judge Policies?

Political science has paid scant attention to the way that citizens judge public policy, assuming that citizens do so, or should do so, in ways familiar to academics themselves, depending upon which of the various schools of thought they endorse. This paper argues that approaching citizens’ judgement realistically requires attention to political psychology. Indeed, our conception of citizen judgement can be enriched by attention to research and theory in cognitive psychology and neuroscience. That work emphasises that much judgement occurs spontaneously and very rapidly, that it is involuntary and non‐semantic and that it depends upon the emotional impact of experience rather than conscious weighing of situations against explicit standards of assessment such as science, self‐interest or moral theory. A moral psychology for public life is sketched out, with implications for judgment by politicians.