磁珠直接吸附法对体态检材游离DNA的提取

目的采用磁珠直接吸附法对人体尿液、唾液、血液3种体态生物检材中的游离DNA进行提取检验,为法医物证中游离DNA的研究及检验工作提供参考。方法对3种生物检材采取离心吸取上清液的方法分离游离DNA,然后采用磁珠直接吸附法进行提取纯化,Identifiler-Plus试剂盒进行复合扩增后常规STR检测。结果在3种检材中均检出了游离DNA,其中血液中游离DNA检出率为100%,唾液为90%,尿液为70%。结论人体体态生物检材中存在游离DNA,同时磁珠直接吸附法可高效、快捷的提取生物检材中的游离DNA。     

STR基因座单倍型的推断方法初探

本文参考Browning SR等提出的以SNP等位基因频率为基础,通过统计学模型推断SNP单倍型的方法,初步探讨亲权鉴定中STR基因座单倍型的推断方法。以两个处于连锁不平衡状态的X-STR基因座的女性分型结果为例进行说明。本方法为STR单倍型的推断提供了一个思路,有助于更科学准确地进行STR分型结果的解释。     

STR遗传多态性调查中样本量和采样方式选择初探

目的对STR基因座进行遗传学调查时样本量大小和采样方式进行考察分析。方法用DNA Typer~(TM)19试剂盒对血卡样本进行直扩,ABI3730型遗传分析仪电泳检测,Genemapper ID v3.2软件进行等位基因分型,根据公式计算群体遗传学参数。计算样本量在50、100、150、200、250、300、350、400、450、500十个水平上遗传多态性水平,并评估与总体的差异。计算常见的四种采样方法(整群采样、随机采样、系统采样、分层采样)所抽取样本遗传多态性水平,并评估抽样误差。结果 18个STR基因座在河北地区16 058份随机样本共检出317种等位基因,基因频率分布在3.114e-5~0.515。18个STR基因座在河北群体水平上PM为6.04e-14,TDP为0.999 999 999 999 94,CEP为0.999 999 987 514 828。PM值、CEP值在样本量大于200后中值基本稳定。四种抽样方法的抽样误差大小是:整群抽样≥单纯随机抽样≥系统抽样≥分层抽样。结论 DNATyper~(TM)19试剂盒的18个STR基因座在河北地区具有较高的遗传多态性水平。在小范围内进行人群频率调查时,可随机选择200份样本代表当地遗传多态性水平。在相对较大范围或遗传背景复杂的区域,可采用分层抽样的方法降低抽样误差。     

河南汉族群体20个常用STR基因座突变分析

目的观察20个常染色体STR基因座突变在河南汉族人群中的分布情况。方法从3011例确认亲子关系的亲子鉴定案例中筛查基因突变事件,确定突变来源,统计各STR基因座的突变率,分析突变规律并与部分不同地区的人群STR基因座突变情况进行比较分析。结果在20个STR基因座中观察到19个基因座的发生的76次突变事件,平均突变率为0.08%累计突变率达到1.662 9%;父、母源性突变的比率大致为8:1;河南汉族人群在Penta E和D12S391基因座突变率明显低于北方汉族人群(P0.05);在D6S1043、CSF1PO和D12S391基因座突变率明显低于广东人群(P0.05);在CSF1PO基因座突变率明显低于云南汉族人群(P0.05)。结论 STR基因座突变现象较为常见,不同基因座的突变率存在着明显的地区差异。     

探求刑事证据规则的和谐

刑事证据规则从其适用的范围看,可分为原则性刑事证据规则与例外性刑事证据规则。原则性刑事证据规则重在保障人权,例外性刑事证据规则重在打击犯罪。刑事证据规则是否和谐,主要体现在能否恰当地处理原则性规则与例外性规则的关系上。和谐的刑事证据规则不仅有利于证据准确适用,而且有利于诉讼和谐和社会和谐。     

A two-level model for evidence evaluation

A random effects model using two levels of hierarchical nesting has been applied to the calculation of a likelihood ratio as a solution to the problem of comparison between two sets of replicated multivariate continuous observations where it is unknown whether the sets of measurements shared a common origin. Replicate measurements from a population of such measurements allow the calculation of both within-group and between-group variances/covariances. The within-group distribution has been modelled assuming a Normal distribution, and the between-group distribution has been modelled using a kernel density estimation procedure. A graphical method of estimating the dependency structure among the variables has been used to reduce this highly multivariate problem to several problems of lower dimension. The approach was tested using a database comprising measurements of eight major elements from each of four fragments from each of 200 glass objects and found to perform well compared with previous approaches, achieving a 15.2% false-positive rate, and a 5.5% false-negative rate. The modelling was then applied to two examples of casework in which glass found at the scene of the criminal activity has been compared with that found in association with a suspect.     

Performance testing of commercial containers for collection and storage of fire debris evidence

Fire debris evidence may contain ignitable liquid residues valuable in the investigation of a potential arson scene. The ability to obtain evidence containers that are contaminant-free and vapor-tight is essential to the analysis and storage of fire debris evidence. Commercial containers such as metal "paint" cans, glass mason jars, and polymer bags are often employed as fire debris evidence containers. The purpose of this research was to determine which of these three types of containers provided the most vapor-tight seal for the prevention of ignitable liquid vapor loss and to assess the potential for cross-contamination. Leak rates for each type of container were measured under controlled conditions. Simple mixtures of hydrocarbons were utilized in these experiments. Leak rates were determined based on the amounts of hydrocarbon recovered from activated charcoal located outside the test container and within a secondary container. Quantitation of the hydrocarbons recovered from activated charcoal was calculated using external standard calibration curves following analysis by gas chromatography-mass spectrometry. The results demonstrated that glass jars had the fastest leak rate followed by metal paint cans and properly heat-sealed polymer bags with the slowest leak rate. Each container exhibited a different leak mechanism, which resulted in an observable effect on the composition of hydrocarbons lost from the container. Hydrocarbon transfer from one container to another is also demonstrated. This study presents results that reveal the most vapor-tight container to be a properly heat-sealed copolymer bag.     

Advanced Framework for Digital Forensic Technologies and Procedures*

Abstract: Recent trends in global networks are leading toward service‐oriented architectures and sensor networks. On one hand of the spectrum, this means deployment of services from numerous providers to form new service composites, and on the other hand this means emergence of Internet of things. Both these kinds belong to a plethora of realms and can be deployed in many ways, which will pose serious problems in cases of abuse. Consequently, both trends increase the need for new approaches to digital forensics that would furnish admissible evidence for litigation. Because technology alone is clearly not sufficient, it has to be adequately supported by appropriate investigative procedures, which have yet become a subject of an international consensus. This paper therefore provides appropriate a holistic framework to foster an internationally agreed upon approach in digital forensics along with necessary improvements. It is based on a top‐down approach, starting with legal, continuing with organizational, and ending with technical issues. More precisely, the paper presents a new architectural technological solution that addresses the core forensic principles at its roots. It deploys so‐called leveled message authentication codes and digital signatures to provide data integrity in a way that significantly eases forensic investigations into attacked systems in their operational state. Further, using a top‐down approach a conceptual framework for forensics readiness is given, which provides levels of abstraction and procedural guides embellished with a process model that allow investigators perform routine investigations, without becoming overwhelmed by low‐level details. As low‐level details should not be left out, the framework is further evaluated to include these details to allow organizations to configure their systems for proactive collection and preservation of potential digital evidence in a structured manner. The main reason behind this approach is to stimulate efforts on an internationally agreed “template legislation,” similarly to model law in the area of electronic commerce, which would enable harmonized national implementations in the area of digital forensics.     

物证鉴定视野下的物质转移理论诠释与定位

物质转移理论起源于20世纪初叶,随着物证鉴定理论与实践的发展和完善,物质转移理论的内涵也不断深化。现代意义上的物质转移理论主要研究客观存在的物体在外力作用下,相互之间发生的物质交换及转移。作为物证鉴定基础理论之一,物质转移揭示出的客观规律对物证技术,尤其是物证鉴定工作具有重要的指导意义。该理论作为诉讼案件中获取鉴定材料的科学基础,其价值不仅在于为确定鉴定方法提供了有力依据,更为物证鉴定提供了重要的理论支撑。     

非法证据的有限排除主义之惑——对《非法证据排除规定》五大理论问题之解读

《关于办理刑事案件排除非法证据若干问题的规定》在对非法证据尤其是非法言词证据的界定、非法证据的证据能力之排除及证明责任的分配、证明标准的设计方面具有开创性的意义。但从该文本在非法实物证据及其排除方面的阙如、对非法言词证据排除在程序设计上的不彻底性等几个方面来看,它所设定的仍是一种对非法证据有保留地排除制度,遵循的其实是非法证据的有限排除主义,因而有进一步完善的必要。