Investigation of Different Ways in Which the CODIS 7.0 May be Used in Mass Disaster Identification

DNA analysis is a key method for the identification of human remains in mass disasters. Reference samples from relatives may be used to identify missing persons by kinship analysis. Different methods of applying the CODIS in disaster victim identification (DVI) were investigated. Two searches were evaluated: (i) relating family relatives to a pedigree tree (FPT) and (ii) relating unidentified human remains to a pedigree tree (UPT). A joint pedigree likelihood ratio (JPLR) and rank were calculated for each search. Both searches were similar in average JPLR and rank. In exceptional cases, namely the existence of a mutation different from the CODIS model, a nonbiological father, a mistake in STR, or incorrect profile association, the UPT search returned one true rank, whereas the FPT search returned no results. This paper suggests a novel strategy to overcome these limitations and increase efficiency in conducting identification of mass disaster victims.     

The Status of Forensic Anthropology in Europe and South Africa: Results of the 2016 FASE Questionnaire on Forensic Anthropology

One of the goals of the Forensic Anthropology Society of Europe (FASE) is to map the existing education and practice opportunities in the field of forensic anthropology in order to support the development of the discipline and to optimize the training courses provided by the Society. To address this goal, an online questionnaire was sent to European and South African practitioners of forensic anthropology and related disciplines in 2016. The results of the questionnaire showed that the status and roles of forensic anthropologists vary depending on the national legal systems, education, and employment status of the practitioners. Despite the fact that the expertise of forensic anthropologists has been increasingly requested in a variety of investigations and the spectrum of tasks has become broader, including identification of living persons, specialized education in forensic anthropology is still restricted to a few graduate and postgraduate programs in European countries and to annual FASE courses.     

Effect of Heat on the Fluorescence Properties of Tooth‐Colored Restorative Materials and Their Forensic Implications

During antemortem and postmortem comparison of dental records of carbonized victims, restorations may be part of such records. The objective of this study was to evaluate the effect of heat on the fluorescence behavior of contemporary tooth‐colored restorative materials and natural tooth structure when subjected to range of temperatures, using illumination with 405 nm wavelength light. A total of 132 human extracted teeth restored with tooth‐colored restorative materials were exposed to heat (200, 500, 900, 1200°C) in an oven for 30 min. Samples were imaged before and after heat treatment. All tooth‐colored restorative materials underwent changes in color and in fluorescence properties, at each of the temperatures used. Resin‐based restorative materials still fluoresced at 200°C, and at 500°C underwent major color changes due to volatilization of resin. Materials containing inorganic fluorophores still fluoresced at 900°C, while at 1200°C, none of the materials tested in this study showed any fluorescence.     

Forensic Facial Approximation: Study of the Nose in Brazilian Subjects

Forensic facial approximation is an auxiliary method for human identification and allows facial recognition. The midface, that includes the nose, is vital for the recognition of a familiar face. The purpose of this study was to set hard tissue parameters to estimate nasal width, to test the method to estimate nasal width of Brazilians, and to analyze the relationship between nasal profile and facial type. A total of 246 computed tomography scans (183 females and 63 males) of adults were analyzed in Horos. Bone tissue measurements and facial type classification were performed on the skull scan. Nasal profile morphology was accessed through the tool 3D surface rendering. There was a difference around 3 mm from real to predicted nose through the method to estimate nasal width in Brazilians. So, the method may be used in forensic practice. Straight nose was associated with long face type.     

Prior knowledge influences interpretations of eyewitness confidence statements: ‘The witness picked the suspect,they must be 100% sure%

When an eyewitness identifies a suspect from a lineup, it is important to know how certain they are about the decision. Even though eyewitnesses are likely to express certainty with words, past research shows that verbal confidence statements (e.g. ‘I’m pretty sure’) are prone to systematic misinterpretation. Until now, no one has examined how an evaluator's prior knowledge, such as which lineup member is the police suspect, influences their interpretation of eyewitness confidence about a lineup identification. Experiments 1 and 3 show that participants perceived the identical statement of confidence as meaning a higher and lower level of certainty, respectively, when the eyewitness's selection either matched or mismatched the police's suspect. Experiment 2 shows that these effects generally persist when the bias manipulation is manipulated between-subjects. Finally, Experiment 3 finds that clarifying the witness's statement with numeric information (e.g. I’m 80% sure) does not eliminate the influence of biasing information.     

Application of HRM-PCR (high resolution melting PCR) for identification of forensically important Coleoptera species

Precise estimation time of death is one of key task of forensic entomology. Especially interesting is Coleofauna present at all stages of cadaver decomposition. The morphological identification of Coleoptera species from varying life stages to species level is time-consuming and needs highly qualified entomologists. Among different molecular methods of species identification very promising is high-resolution melting PCR. It allows fast single-tube assignment of analyzed sample to species based on amplicon melting profile. The object of this study were different specimens of Coleoptera collected at pig cadavers in Łomna (central Poland) during 2012 - 2014. Specimes were identified to species by experts of corresponding Coleoptera families. From 120 collected specimens belonging to four families and twelve species HRM-PCR correctly identified specimens belonging to three families and eight species.     

指纹鉴定技术在痕迹检验工作中的运用探析

指纹鉴定技术作为痕迹检验技术中的一项基本内容,是现场勘查工作中的重要技术手段。虽然近年来我国不断加强指纹鉴定方面的技术开发与应用,但在指纹识别、比对等方面的效率还比较低。基于此,有必要针对指纹鉴定技术在痕迹检验工作中的运用进行研究。在对指纹鉴定技术进行概括介绍的基础上,就指纹鉴定技术的运用现状进行分析,并对如何优化技术的运用提出相关的策略建议。     

Contactless Visualization of Latent Fingerprints on Nonporous Curved Surfaces of Circular Cross Section—A Statistical Evaluation on the Materials as Plane Mirror

A new contactless technique for latent fingerprint visualization on nonporous curved surfaces of circular cross section was introduced by Low et al. (1). The technique utilizes a plane mirror to convey the light rays toward the inspected surfaces for latent fingerprint visualization. This research activity came up as an extension of the previous study which utilized an aluminum plate as the plane mirror to illuminate the inspected surfaces. Dulling spray was used to increase the diffuse component of the reflective aluminum plate. However, the amount of dulling spray will affect the uniformity of the illumination on the inspected curved surfaces. In this study, a study on the new materials for the plane mirror was carried out. Coated aluminum, opal, and ground glass diffusers were selected as the new materials. The performance of the new materials was compared to the aluminum based on the quality of the captured images on various nonporous cylindrical surfaces. A statistical approach known as randomized complete block design was used to design the experiment. The quality of the captured images was obtained using Spectral Image Validation and Verification. Two‐way analysis of variance and Fisher's least significant difference test were used to analyze the quality of the images. From the results of the statistical analysis, coated aluminum has the best performance compared to aluminum, opal, and ground glass diffusers.     

From unknown to known: Identification of the remains at the mausoleum of fosse Ardeatine

During the Second World War, on 24th March 1944, 335 Italians were massacred near Rome by the occupying forces of Nazi Germany. Four months later forensic examination led to the identification of 323 out of 335 victims. After approximately 60?years, the identification of the remaining unidentified twelve victims began with anthropological and genetic analysis carried out by a team of Italian forensic experts. Anthropological analysis was performed in field in order to confirm the sex of each victim and verify the presence of only one individual in each grave for a correct sampling. Selected bone fragments for each individual were then collected and transferred to the laboratory for genetic analysis. Although the anthropological ante mortem information was limited, morphological and metrical data was collected for a possible future identification of the victims. Subsequently, the typing of autosomal loci, Y-STR and mtDNA D-loop region of all bone and available reference samples was conducted. LR and cumulative LRs obtained from autosomal STR and Y-STR results confirmed the alleged relationship between three victims and their relatives with values over 10⁴ (one sample) and 10⁶ (two samples). Therefore, the genetic analysis offered the families the possibility of replacing the number of the grave with the name of the victim.