根据骨盆CT片特征指标进行同一认定的研究

目的选择骨盆CT片上的特征指标,建立逐步回归方程,探讨其在法医学同一认定中的应用价值。方法收集160名不同被检查者骨盆CT影像片各1张,70名被检查者不同次骨盆CT影像片各2张。选择并测量骨盆CT片上的14项指标值,分别计算不同人随机分组相同测量指标的组间的差值,以及相同人不同次测量指标间的差值,运用二分类logistic逐步回归分析,建立各项指标的一元回归方程和多项指标的多元回归方程,并对方程进行盲测检验。结果建立的14个一元方程中同一认定的正确率在61.1%(骶骨耳状面后缘宽)~80.5%(第一骶椎平面左右髂骨前端间距)之间;建立的6个多元回归方程的正确率在80.5%~93.8%之间。盲测准确率为100%。结论本文在CT片上选择的14项特征指标可以用于同一认定,在使用时应尽可能选用多元指标以得到更准确的结果。     

空肠弯曲杆菌生物膜的体外形成及鉴定

采用结晶紫染色与正交试验相结合的方法,对空肠弯曲杆菌在不同温度、pH值、气体条件以及不同培养基条件下形成生物膜的能力进行了检测和比较;并以筛选出的最佳培养条件在盖玻片上形成的生物膜为对象,用光学显微镜及扫描电镜观察生物膜的结构。结果显示,在正常气体条件下培养1～3d,生物膜形成水平明显高于其他2种气体条件(P<0.01),4d后3种气体条件下的生物膜形成量均有增加,但3组间差异不显著,说明正常空气组分的条件更有利于生物膜的快速形成,而且37℃、正常空气条件下,在pH7.0的MH肉汤中培养5d,通过显微镜和扫描电镜观察,在盖玻片上可形成典型的、可观测的生物膜结构。     

貂源铜绿假单孢菌的分离鉴定及其致病性研究

为确定山东威海某养殖场发病水貂的病原菌,从发病水貂肺中分离并纯化细菌,根据其形态特征、培养特性、生化试验结果及PCR鉴定,确定其为铜绿假单孢菌。致病性试验中,将8.0×1010CFU/mL的菌液10倍系列稀释后,感染60日龄健康昆明系小鼠,半数致死量和最小致死量分别为1.4×107 CFU/mL和8.0×106 CFU/mL。剖检死亡小鼠可见皮下和肺出血,并能从死亡小鼠的肺中分离出与感染菌株形态特征完全一致的菌落。药敏试验结果显示,该分离菌株对庆大霉素、环丙沙星、诺氟沙星、头孢哌酮等较为敏感,而对青霉素、链霉素不敏感。系统发育分析结果表明,该分离菌株与从小麦中分离的铜绿假单孢菌的亲缘关系最近,据此怀疑该发病水貂有可能是食用了污染的饲料引起的。     

兔F型多杀性巴氏杆菌的分离鉴定及其致病性

对从山东某獭兔养殖场病死仔兔肺中分离的1株疑为巴氏杆菌菌株进行了菌落形态学观察及培养特性、革兰氏染色、生化反应、仔兔致病性回归试验、小鼠毒力试验,并采用PCR方法对该分离株进行了种属及荚膜血清型鉴定。结果显示,该分离菌株革兰氏染色阴性,生化试验及PCR鉴定结果显示为F型多杀性巴氏杆菌,能引起獭兔仔兔肺出血、坏死,并致部分仔兔死亡。表明该分离菌株为比较少见的兔F型多杀性巴氏杆菌。     

我国民事诉讼标的及其识别问题分析

民事诉讼标的是指法院予以审理和裁判的对象。我国的民事诉讼标的理论吸收大陆法系诉讼标的理论基础,形成民事纠纷说、新二分支说、分别识别说与为实践部门普遍接纳的旧实体法说等诸学说。其争议焦点主要围绕给付之诉的诉讼标的识别标准进行。从利于诉讼实践的角度出发,仍应坚持实体法学说,但诉讼标的的识别要以原告向法院提出的实体法律关系或请求权为准,未向或未能向法院提出的实体法律关系或请求权不能作为识别依据。     

从龚如心巨额遗产继承案看我国文件鉴定工作的完善

应尽快制定全国统一的《文件检验鉴定程序规则》;组织制定全国统一的《文件检验鉴定技术标准》;进一步规范文件检验鉴定文书的制作;强化文件检验鉴定人出庭作证的意识,提高出庭作证的技巧;正确认识文件检验鉴定结论的作用,不能单以鉴定结论决定判决结果。     

两株乙型脑炎病毒的分离鉴定与生物学特性研究

2009年从四川多个地区的猪场采集流产死胎及库蚊样本12份,采用BHK-21细胞培养方法分离出2株乙型脑炎病毒,分别命名为JEV-CZ1与JEV-YA1,PrM/C基因与疫苗株SA-14-14-2的同源性分别为90.9%与99.6%,基因分型研究显示JEV-CZ1株为基因Ⅰ型乙型脑炎病毒,JEV-YA1株为基因Ⅲ型。分离株在BHK-21细胞中能产生明显的细胞病变,其细胞半数感染量(TCID50)分别为10-6.206 TCID50/0.025mL与10-4.943 TCID50/0.025mL。经脑内注射测得分离株对体重9～11g小鼠的半数致死量(LD50)分别为9.97LD50/0.1mL和6.95LD50/0.1mL,其中JEV-CZ1株毒力较强。小鼠接种分离株3～4d后出现抽搐、肢体痉挛、异常兴奋等神经症状,5～7d后全部死亡,剖检可见脑部充血、水肿;病理切片观察可见脑内神经细胞变性坏死,其他器官也出现不同程度的病变。结果表明这2个分离株符合乙型脑炎病毒的特征。     

Mononationals,hyphenationals, and shadow-nationals: multiple citizenship as practice

Multiple citizenship has in recent decades moved from an unwanted phenomenon in international relations to a fairly common transnational status. Multiple citizenship has nevertheless so far been studied mainly as a political and juridical status by comparing national legislations. Much less notice has been given to actual dual citizens' citizen participation and construction of citizens' identities. Only when citizenship is studied as these kinds of practices do the hypothetic possibilities and problems associated with the status get their meanings and contents. This paper concentrates on examining dual citizens' identifications to their respective citizenships and how these affiliations transfer into possible citizen participation. Results are based on extensive analysis of survey (n = 335) and interviews (n = 48) carried out among dual citizens living in Finland. Contents and forms of dual citizens' national identification and citizen participation were reviewed through ideal types: resident-mononationals, expatriate-mononationals, hyphenationals, and shadow-nationals.     

mtDNA多态性在嗜尸性昆虫种类鉴定中的应用

嗜尸性昆虫的种类鉴定是利用昆虫学知识进行法医学研究的重要步骤之一.近年来研究表明,利用分子生物学技术,特别是线粒体DNA(mitochondrial DNA,mtDNA)序列分析技术对嗜尸性昆虫进行种类鉴别,能够得到较好的效果.本文对嗜尸性昆虫mtDNA的分子生物学特性、结构以及嗜尸性昆虫种类鉴定中常用的mtDNA序列...     

Positive identification by a skull with multiple epigenetic traits and abnormal structure of the neurocranium, viscerocranium, and the skeleton

Wormian bones are small ossicles appearing within the cranial sutures in more than 40% of skulls, most commonly at the lambdoid suture and pterion. During the skeletal analysis of an unidentified male war victim, we observed multiple wormian bones and a patent metopic suture. Additionally, the right elbow was deformed, probably as a consequence of an old trauma. The skull was analyzed by cranial measurements and computerized tomography, revealing the presence of cranial deformities including hyperbrachicrania, localized reduction in hemispheral widths, increased cranial capacity, and sclerosis of the viscerocranium. Besides unique anatomical features and their anthropological value, such skeletal abnormalities also have a forensic value as the evidence to support the final identification of the victim.