Detection of microRNAs in DNA Extractions for Forensic Biological Source Identification

Molecular‐based approaches for biological source identification are of great interest in the forensic community because of a lack of sensitivity and specificity in current methods. MicroRNAs (miRNAs) have been considered due to their robust nature and tissue specificity; however, analysis requires a separate RNA extraction, requiring an additional step in the forensic analysis workflow. The purpose of this study was to evaluate miRNA detection in blood, semen, and saliva using DNA extraction methods commonly utilized for forensic casework. RT‐qPCR analysis revealed that the tested miRNAs were consistently detectable across most tested DNA extraction methods, but detection was significantly reduced compared to RNA extracts in some biological fluids. DNase treatment was not necessary to achieve miRNA‐specific results. A previously developed miRNA panel for forensic body fluid identification was evaluated using DNA extracts, and largely demonstrated concordance with results from samples deriving from RNA extracts of semen, blood, and saliva.     

Validation of the 4‐aminophenol color test for the differentiation of marijuana‐type and hemp‐type cannabis

The analysis of cannabis plant material submitted to seized‐drug laboratories was significantly affected by the signing of the Agricultural Improvement Act of 2018, which defined hemp and removed it from the definition of marijuana in the Controlled Substances Act. As a result, field law enforcement personnel and forensic laboratories now are in need of implementing new protocols that can distinguish between marijuana‐type and hemp‐type cannabis. Colorimetric tests provide a cost‐effective and efficient manner to presumptively identify materials prior to submission to a laboratory for analysis. This work presents the validation of the 4‐aminophenol (4‐AP) color test and demonstrates its utility for discriminating between marijuana‐type and hemp‐type cannabis (i.e., typification). Validation studies included the testing of numerous cannabinoid reference materials, household herbs, previously characterized cannabis plant samples, and real‐case samples. The 4‐AP test reliably produces a pink result when the level of Δ⁹‐tetrahydrocannabinol (THC) is approximately three times lower than the level of cannabidiol (CBD). A blue result is generated when the level of THC is approximately three times higher than that of CBD. Inconclusive results are observed when the levels of THC and CBD are within a factor of three from each other, demonstrating the limitations of the test under those scenarios.     

Character Weakness,Partisan Bias,and Presidential Evaluation: Modifications and Extensions

In a recent article Goren (American Journal of Political Science, 46, 627–641, 2002) draws upon theories of negativity bias, partisan bias, and motivated reasoning to posit that the more strongly people identify with the opposition party of a presidential candidate, the more heavily they will rely on character weakness impressions to construct global candidate evaluations. This paper modifies the theoretical framework by positing that (1) partisans will judge opposition nominees most critically on the traits owned by the former’s party and (2) partisan bias promotes negativity bias in the evaluation of incumbent presidents seeking reelection and incumbent vice presidents seeking the presidency. Analysis of data from the 2000 and 2004 NES surveys, along with a reconsideration of the results from the 1984 to 1996 period covered in the original piece, yields strong empirical support for these expectations.

Explaining Turnout Decline in Britain, 1964–2005: Party Identification and the Political Context

Turnout decline in Britain is greater than it first appears since changes in the social composition of the electorate have had a positive impact on turnout. This paper finds that whereas a weakening in the strength of party identification is associated with the long-term decline, the political context influences short-term variation. Partisan dealignment is also changing the dynamics of the determinants of turnout. Since non-identifiers are more strongly influenced by the political context than strong identifiers, and there are now more non-identifiers than previously, the political context is becoming a more important factor in determining whether people vote or not.

Identification and analysis of human remains recovered from wells from the 1991 War in Croatia

From 1996 to the present, the remains of 61 individuals killed during the 1991 War in Croatia were recovered from both dried out and functioning wells. Positive identification was established in 60.7% or 37/61 cases. Remains recovered from the same geographical region but from non-well settings were identified in 77.4% or 1256/1623 cases. The purpose of this paper is to report on the taphonomic, demographic and trauma characteristics of remains recovered from wells and identify factors responsible for the discrepancy in the identification ratios. The age and sex distributions in the well and non-well series were similar, as were the frequencies of recovered personal documents, jewelry and other artifacts. The taphonomic features of the remains were, however, significantly different. Preservation of remains was considerably better in non-well settings (1400/1623 or 86.3% compared to 40/61 or 65.5% in wells). Congruently, commingling of remains was more frequent in wells (26/61 or 42.6% compared to 77/1623 or 4.7% in non-well settings). In bodies recovered from non-well settings the preservation, state and commingling of the remains were strongly correlated with positive identification. None of these features were correlated with the identification of bodies from wells. Instead, identification of remains from wells was significantly affected by the presence or absence of water in the well. As both series have similar frequencies, and identical rankings of identification factors, the reason for the discrepancy in the identification ratios lies in the fact that by themselves, these factors were rarely sufficient for positive identification. In both series the majority of identifications (51.4% in the well, and 58.1% in the non-well series) were established through a combination of biological and non-biological features. The significantly lower identification ratio in the well series resulted from the difficult recovery conditions in wells with significant amounts of water, and the negative effect that water had on the preservation of clothing, personal artifacts and some dental features. Significant differences were also noted in the types and locations of perimortem traumas. There is a significantly higher frequency of entrance gunshot wounds to the back of the head, suggestive of execution style murders, in the well series.     

流产的病因及其法医学鉴定中应注意的问题

流产的病因复杂多样,常见的原因有:遗传基因的异常、免疫疾病、内分泌异常、精子异常等。其他如感染、生殖器官疾病、全身性疾病、环境因素、外伤及其精神刺激与手术等也会导致流产。在法医学鉴定中,对于外伤后流产应排除非外伤性流产因素后,才能认定外伤性流产。     

计算机自动化笔迹鉴别可行性分析

当前,以计算机技术为代表的高新技术的发展,为重新认识笔迹特征提供了新的手段.通过对笔迹鉴定理论的再认识,对计算机自动化笔迹鉴别的各环节进行深入分析,探讨计算机自动化笔迹鉴别的可行性,具有重要的实践价值.     

司法鉴定与刑事侦查中同一认定理论的比较与融合

同一认定理论是司法鉴定与刑事侦查领域中的重要基础理论。文章从司法鉴定和刑事侦查领域中同一认定理论的运用入手,分析了两者在同一认定的主体、方法手段、适用程序方面的差异性,指出了司法鉴定与刑事侦查中的同一认定理论融合的意义与完善措施。     

Differentiating human bone from animal bone: a review of histological methods

This review brings together a complex and extensive literature to address the question of whether it is possible to distinguish human from nonhuman bone using the histological appearance of cortical bone. The mammalian species included are rat, hare, badger, racoon dog, cat, dog, pig, cow, goat, sheep, deer, horse, water buffalo, bear, nonhuman primates, and human and are therefore not exhaustive, but cover those mammals that may contribute to a North American or Eurasian forensic assemblage. The review has demonstrated that differentiation of human from certain nonhuman species is possible, including small mammals exhibiting Haversian bone tissue and large mammals exhibiting plexiform bone tissue. Pig, cow, goat, sheep, horse, and water buffalo exhibit both plexiform and Haversian bone tissue and where only Haversian bone tissue exists in bone fragments, differentiation of these species from humans is not possible. Other primate Haversian bone tissue is also not distinguishable from humans. Where differentiation using Haversian bone tissue is undertaken, both the general microstructural appearance and measurements of histological structures should be applied. Haversian system diameter and Haversian canal diameter are the most optimal and diagnostic measurements to use. Haversian system density may be usefully applied to provide an upper and lower limit for humans.     

Allele frequencies of 20 Y-chromosomal short tandem repeat loci in a tribal population of Deccan Plateau, India

Population: Eighty male individuals from a nomadic tribal population belonging to Dravidian and Indo-Caucasian ethnicities from Deccan Plateau, Andhra Pradesh, India, were analyzed in the present study.