Detection of Gamma-Hydroxybutyric Acid in Various Drink Matrices via AccuTOF-DART*

Abstract: A new screening method for detecting gamma-hydroxybutyric acid (GHB) in drink matrices, using the IonSense, Inc. (Saugus, MA) direct analysis in real time (DART) ion source coupled to a JEOL exact mass time-of-flight mass spectrometer (AccuTOF), was validated and compared with the current screening methodology. The DART ion source allows for analysis of samples under ambient conditions with little to no sample preparation. Fifty drink specimens were spiked at levels of 1, 2, 3, and 4 mg/mL GHB, and analyzed on the AccuTOF-DART. Positive detection of GHB occurred for each of the samples at each concentration level, giving 100% accuracy for the samples tested. Twenty-five of the 50 drink specimens were spiked at 1 mg/mL GHB and tested using a color test known as the GHB Color Test #3. Only two of these 25 specimens tested positive for the presence of GHB, giving only 8% accuracy. Implementation of this new methodology as a screening tool for GHB analysis will quickly eliminate negative specimens allowing the examiner to focus analysis time on those that screened positive.     

SPE-HPLC法分析大鼠血浆中灭多威

目的建立以固相萃取-高效液相色谱（SPE-HPLC）分析大鼠血浆中灭多威的方法。方法以空白大鼠血浆添加灭多威标准品及内标物,以SPE法对样品进行前处理,采用HPLC进行分析,考察仪器测试条件、特异性、回收率、灵敏度、精密度、准确度、线性关系、稳定性,并对方法进行优化。结果采用本文方法测得灭多威在血浆中的线性范围0.1-20μg/mL（r=0.9993,P〈0.001）,血浆中的检测限0.03μg/mL（S/N≥3）,日内、日间精密度的CV值分别在8.33%与11.11%以内,日内、日间准确度值分别在90%与120%之间,回收率值在88%±4.4%以上。结论所建方法实用、便捷,可对血浆中的灭多威进行定性定量地快速测定。     

Suicide by Cop Among Officer-Involved Shooting Cases

Abstract: The frequency and characteristics of suicide by cop cases (SBC) among a large (n = 707) nonrandom sample of North American officer-involved shootings (OIS) were investigated. “Suicide by cop” is when a subject engages in behavior which poses an apparent risk of serious injury or death, with the intent to precipitate the use of deadly force by law enforcement against the subject. Thirty-six percent of the OIS in this sample were found to be SBC with high interrater agreement (intraclass correlation coefficient = 0.93) for category classification. SBC cases were more likely to result in the death or injury of the subjects than regular OIS cases. Most SBC cases were spontaneous, but had clear verbal and behavioral indicators that occurred prior to, and during the event. Findings confirm the trend detected in earlier research that there was a growing incidence of SBC among OIS. SBC individuals had a high likelihood of possessing a weapon (80%), which was a firearm 60% of the time. Half of those with a firearm discharged it at the police during the encounter. Nineteen percent simulated weapon possession to accomplish their suicidal intent. Other findings highlight the histories and commonalities in this high risk group.     

民事诉讼中司法会计鉴定意见及其质证

司法会计鉴定意见虽然是一种法定的证据形式，但不具有天然的“权威性”，在民事诉讼中需要在法庭上经过质证才能确定其证明能力和证明力。从民事诉讼角度，论述司法会计鉴定意见及其质证问题，分析司法会计鉴定意见质证的现状及存在的问题，并提出完善司法会计鉴定意见质证的思考。     

论鉴定留置的若干法律问题

鉴定留置指为鉴定被告心神或者身体状态，而将被告送入医院或者其他适当处所之处分。鉴定留置的存在是与法医学的发展休戚相关。从某种意义上讲，现代去医学发展的不成熟是鉴定留置制度存活的土壤。鉴定留置应定性为一种依附性行为。即依附于强制鉴定，其本身属于调查程序。而非强制处分。鉴定留置属于强制鉴定延伸的自然结果．而强制鉴定将在一定程度上限制被告人的行动自由。应当接受司法审查。强制鉴定作为一种独立的强制措施．对自由的侵犯程度不可与羁押同日而语，甚至其结果可能减轻或者免除被告人的刑事责任。因此在司去审查控制下的鉴定留置期间不应当计入羁押期间。     

112例脑弥漫性轴索损伤分析

目的探讨脑弥漫性轴索损伤（diffuse axonal injury,DAI）与脑挫裂伤和原发性脑干损伤的关系。方法分析112例DAI伤者的法医临床学资料和影像学特点,对原发性脑损伤的特征进行比较。结果112例DAI伤者中70.5%为交通事故致伤,多次暴力致伤比较多见（60.7%）,伴脑挫裂伤者80例（71.4%）。CT或MRI发现出血灶者90例。结论DAI多伴有脑皮质挫裂伤和原发性脑损伤,CT或MRI有助于法医学死因分析和伤残程度鉴定。     

司法会计鉴定与文书鉴定配合运用的效果

司法会计鉴定和文书鉴定的配合运用不仅有助于提高鉴定效率。增强鉴定的准确性和针对性，而且有助于开拓思路，发现新的线索。     

论司法鉴定意见的基本属性

本文分析司法鉴定意见的基本属性，认为其同时具备科学证据以及证据学的特征属性．在此基础上针对法医学鉴定文书存在的问题。就法医学鉴定文书的制作方法进行探讨。     

对邱兴华杀人案的司法鉴定学反思

关于邱兴华杀人案（下称邱案）的各种学术讨论和争论似乎已经不少，但查阅文献发现，有关邱案的文章，分别是从刑事诉讼法学、刑事证据学、刑事心理学、精神医学角度，法制新闻学以及从人权保护，法治进步等角度对该案进行解读。然而既然这是一个涉及司法鉴定的个案（虽然实际上并没有进行任何实质性鉴定活动），那么从司法鉴定学的角度对与该案有关的几个最基本的问题进行理论阐述，或许能有些许不同的作用。这几个问题包括：精神病概念的辨析，是否患病与是否需要接受刑事处罚之间是什么关系，何时需要启动司法精神医学鉴定？应如何配置我国刑事诉讼中的司法精神医学鉴定启动模式？     

Developmental Validation of the Quantifiler® Duo DNA Quantification Kit for Simultaneous Quantification of Total Human and Human Male DNA and Detection of PCR Inhibitors in Biological Samples*

Abstract: The Quantifiler^® Duo DNA Quantification kit enables simultaneous quantification of human DNA and human male DNA as well as detection of inhibitors of PCR in a single real-time PCR well. Pooled human male genomic DNA is used to generate standard curves for both human (ribonuclease P RNA component H1) and human male (sex determining region Y) specific targets. A shift in the cycle threshold (C_T) values for the internal positive control monitors the presence of PCR inhibitors in a sample. The assay is human specific and exhibits a high dynamic range from 0.023 to 50 ng/μL. In addition, the multiplex assay can detect as little as 25 pg/μL of human male DNA in the presence of a 1000-fold excess of human female DNA. The multiplex assay provides assessment of the DNA extract and guidance for the selection of the appropriate AmpFℓSTR^® Amplification Kit to obtain interpretable short tandem repeat profiles.