五四青年教育探析——新文化运动倡导者的青年教育特点

在五四爱国运动中发挥了重要作用的五四青年的成长,离不开新文化运动倡导者的教育培养。新文化运动倡导者在五四青年教育实践中,体现出:充分信任青年,寄希望于青年,重视实现青年价值;以平等对话的方式,导引知识,启发青年思考,帮助青年认识真理、发现真理,重视唤起青年自觉;鼓励青年积极作为,热情支持青年行动,身先士卒,重视引领青年践行等三个方面的特点。     

Pampered Sons, (Wo)manly Men,or Do‐nothing Machos? Costa Rican Men Coming of Age under Neoliberalism

This article explores how young men in Costa Rica negotiate ideas of manhood under neoliberalism. We draw on interview data involving 23 men, ages 15–35, residing in one Costa Rican city. Comparing men across three different class locations, we find diverse "markers of manhood." Our data suggest an emerging globally dominant masculine ideal among an elite class of men, a declining locally dominant masculine ideal among working-class men, and a cynical, possibly counter-cultural masculine ideal among poor men. We conclude that masculinities are not only fluid, but tied to changing economic circumstances and class structures.     

牛趋化因子受体4基因的克隆表达及序列分析

采用RT-PCR技术,从被刺激诱导的牛外周血单个核细胞(PBMC)中克隆了牛趋化因子受体4(CXCR4)全长基因,并将CXCR4基因和CXCR4基因N端1～126 bp(CXCR4~(42aa))片段分别亚克隆到原核表达载体pGEX-4T-1中,命名为pGEX-4T-1-CXCR4、pGEX-4T-1-CXCR4~(42aa).获得重组菌株后,采用不同的IPTG浓度、不同诱导时间、不同温度和不同培养基等组合诱导表达这两种重组菌,其中pGEX-4T-1-CX-CR4~(42aa)重组菌获得了表达,并在37℃、IPTG浓度为0.8 mmol/L、诱导6 h时表达量最大,约占菌体总蛋白的45%,目的蛋白主要以包涵体的形式存在;而pGEX-4T-1-CXCR4重组菌未表达出目的蛋白.     

日本平台经济下的“类雇员”概念建构及其启示

平台经济在各国的发展状况不同,各国对其涉及的劳动关系认定等问题进行规制的法律制度也不同。日本平台经济下的劳动关系认定相关探讨建立在个别法与集体法对劳动者概念之界定、相关的判例和学理的基础上,主要思路是修订现行《家内劳动法》与导入德国"类雇员"概念。在充分考虑日本对于平台经济之定位与我国有所区别的前提下,其兼顾"如何划定适用对象"及"对其采用何种规制"的政策研究方式,以及构建"类雇员"概念的相关探讨,对我国相关政策研究及法律制定极具借鉴意义。     

Potential Forensic Application of Receptor for Advanced Glycation End Products (RAGE) as a Novel Biomarker for Estimating Postmortem Interval

Studies which estimate the postmortem interval (PMI) based on diatoms or bacterial communities have been well‐conducted. However, predicting the PMI based on microorganisms has limited accuracy. The objective of this study was to discover specific biomarkers for estimating the PMI for drowning. The in vivo experimental model consisted of rats classified into nine groups (seven drowned groups from Day 1 to Day 7 and two control groups). Their lung tissues were analyzed for the receptor for advanced glycation end products (RAGE) mRNA expression determined by qRT‐PCR. RAGE protein levels were determined by Western blotting and immunohistochemical (IHC) staining. RAGE mRNA and protein levels gradually degraded over time. Immunostaining analysis revealed that RAGE in the Day 1 sample was significantly higher than that in the Day 6 or Day 7 samples. These results indicate that RAGE in drowned lungs could be a potential biomarker for the determination of the PMI after drowning.     

Informing the Understudied R: Exploring and Advancing Typological Research

Creating offender typologies has become a growing interest and a potential method of improving understanding of programming needs and potential placements. Most typological research has only explored and described potential offender types. Relatively few studies have attempted to confirm the existence of created typologies or examine how offenders in each type predict important outcomes. Utilizing a large sample (N = 37,111) of reentering male offenders from Washington State Department of Corrections, we computed both an exploratory and confirmatory latent class analysis. After the confirmation of six offender types, we examined each type’s likelihood of several recidivistic outcomes. We anticipate that the described typology will assist case management, prioritizing offender needs, optimizing treatment services, and determining sequencing of multiple treatment types.     

大熊猫轮状病毒CH-1株外衣壳蛋白VP4基因的克隆与生物信息学分析

利用MA-104细胞培养增殖大熊猫轮状病毒CH-1株,提取总RNA,运用RT-PCR扩增外衣壳蛋白VP4基因,将其与pMD19-T simple vector连接并转化DH5α,经PCR扩增和测序分析进行鉴定,用生物信息学软件预测其功能,并构建系统进化树。结果显示,成功获得大熊猫轮状病毒VP4蛋白基因,长2 362bp,包含一个2 331bp的开放阅读框,编码776个氨基酸,测序结果在GenBank中的登录号为HQ641296。生物信息学分析表明,VP4蛋白基因编码产物分子质量为86 768.8u,理论等电点为5.56,半衰期为30h(体外哺乳类网状细胞),不稳定系数为28.64,脂肪指数为82.53,总平均亲水性为-0.265,最大疏水性为2.244,最小疏水性为-2.911;无信号肽序列;跨膜结构分析显示VP4蛋白无跨膜区,位于病毒膜外区;在VP4序列中的抗原决定簇主要位于VP4的N-末端和C-末端。预测其可能包含8个N-糖基化作用位点,15个蛋白激酶C磷酸化作用位点、15个酪蛋白激酶Ⅱ磷酸化作用位点、12个N-豆蔻酰化位点。系统进化分析显示,大熊猫轮状病毒的VP4基因与猪轮状病毒的VP4基因的进化距离最...     

警务战术课堂教学的和谐互动策略

警务战术课堂教学应是和谐互动的,它使“战术培训的教”与“战术培训的学”产生谐振效应,从而提高警务战术课的教学效果,真正促进学生的警务战术理论和应用能力的全面和谐的发展。     

英语课堂提问策略

课堂提问一直是英语教学的重要组成部分,恰当的课堂提问可以激发学生的学习兴趣和创造力,使学生成为课堂的主体。然而目前我国外语教学课堂提问所存在的问题却不容忽视,本文就存在的五个问题,根据衡量提问有效性的标准分别论述了所应采取的解决办法,使课堂提问真正成为开发学生思维,提高学生创新能力的主渠道。     

浅谈文学翻译中的审美认识

文中从文艺美学的角度探讨了文学翻译中美的认识、挖掘和再现,翻译工作者应具备强烈的文学审美意识,提高翻译的质量。