窖蛋白与不明原因心源性猝死的相关性研究进展

不明原因心源性猝死(unexpected sudden cardiac death,USCD)因其不伴有心脏结构的异常,尸体解剖呈阴性改变,一直是法医病理学鉴定的热点难题。USCD可能与部分致死性心律失常有关,该类心律失常多由心脏离子通道蛋白或其相关蛋白发生异常所致。窖蛋白可以通过其脚手架区域与多种心肌离子通道蛋白结合,在维持心肌动作电位的去极化和复极化中起到关键作用。当窖蛋白由于基因突变或蛋白表达异常等因素导致其结构和功能受到影响时,受其调控的心肌离子通道的功能也受到损害,继而引起多种离子通道病的发生,出现心律失常甚至心源性猝死。研究窖蛋白对离子通道功能的影响对于探索恶性心律失常及心源性猝死的发生机制具有重要意义。     

Rapid screening for the detection and differentiation of γ-hydroxybutyrate using ion chromatography

The analysis of gamma-hydroxybutyric acid (GHB) is problematic because it is hygroscopic, it lacks a good UV chromophore, and it undergoes heat-induced cyclization. This paper presents a new method utilizing ion-exchange chromatography (IC) with conductivity detection. The simple sample preparation, rapid analysis time, and inorganic anion detection capabilities are all advantages over the current methods. The detection of inorganic salts (formed during GHB synthesis) gives insight into the synthetic route utilized and can aid in drug seizure comparison. The developed method has a detection limit for GHB anions of 0.57 mg/L and chloride of 0.22 mg/L. A comparison of this technique with a current gas chromatography-mass spectrometry technique is presented, and a t-test found that the two methods' results are not statistically different at the 99.9% confidence level demonstrating the merits of this fast, simple, and informative IC method as a routine screening tool.     

Analysis of forensic soil samples via high-performance liquid chromatography and ion chromatography

Traditional forensic soil comparisons are performed via physical and/or chemical examinations of color, texture, and mineral content, leaving any organic- or water-soluble fractions unexamined. This study uses high-performance liquid chromatography (HPLC) and ion chromatography (IC) to assess the qualitative and quantitative variation in these fractions of soil. Soil samples (n=120) were collected over the course of 3 weeks from urban, suburban, and rural locations in and around Lansing, MI. Additional samples from six of these locations (two urban, two suburban, and two rural) were collected once a week for 10 weeks for temporal analysis. Nine additional samples, equally spaced over a 1 m(2) grid, from these same six locations were collected for spatial analyses. Qualitative and quantitative analysis of the resultant chromatograms separated the 120 samples into 10 groups by HPLC and 23 groups by IC. This study shows that using HPLC and IC to analyze the organic- and water-soluble fractions of soil can successfully discriminate samples. Quantitative analysis of the results eliminates some false inclusions by providing further differentiation of samples. The results of this study indicate that adding HPLC and IC analyses to traditional forensic soil analysis schemes can improve overall sample differentiation. The methods used in this study were also able to detect both qualitative and quantitative variations in soil over a relatively small geographic area. This demonstration of soil heterogeneity underscores the importance of the collection of a representative known sample population when assessing a forensic soil comparison. Significant temporal variation was also demonstrated over the course of 10 weeks of sampling; however, samples were found to be consistent over shorter periods of time. Baseline levels of inorganic anions were determined via IC; these levels may be useful in assessing the significance of anions detected in soil from cases involving low explosives.     

Transfer of Triazine‐iron(II) Chromic Complexes Left by Iron Items on Textile Background and Human Skin

Abstract: The research is focused on the detection and transfer of iron traces left by iron items on clothing and human skin. The method is based on the formation of colored complexes between ferrous ions and five synthesized, mostly new triazines. Iron traces originally were left by iron rings on slightly wetted (artificial sweat) cotton fabrics and subsequently transferred to a separate textile substrate. Prior to the use of trazines the contact spots were treated with a new inorganic reducing agent (Sn²⁺) to reduce Fe³⁺ to Fe²⁺. The method is sensitive to detect iron traces on wetted canvas after 10 min contact with iron items. More spectacular results were obtained for traces left on human palm even after very short contact (10 sec). The new iron‐trace‐transfer method eliminated the contact of triazines solutions with human skin. Transmission visible spectra of Fe(II)–triazine complexes were determined.     

Using Gas Chromatography with Ion Mobility Spectrometry to Resolve Explosive Compounds in the Presence of Interferents*

Abstract: Ion mobility spectrometry (IMS) is a valued field detection technology because of its speed and high sensitivity, but IMS cannot easily resolve analytes of interest within mixtures. Coupling gas chromatography (GC) to IMS adds a separation capability to resolve complex matrices. A GC‐IONSCAN^® operated in IMS and GC/IMS modes was evaluated with combinations of five explosives and four interferents. In 100 explosive/interferent combinations, IMS yielded 21 false positives while GC/IMS substantially reduced the occurrence of false positives to one. In addition, the results indicate that through redesign or modification of the preconcentrator there would be significant advantages to using GC/IMS, such as enhancement of the linear dynamic range (LDR) in some situations. By balancing sensitivity with LDR, GC/IMS could prove to be a very advantageous tool when addressing real world complex mixture situations.     

Movement-space: The changing domain of thinking resulting from the development of new kinds of spatial awareness

This paper is an attempt to describe the nature of a new calculative background that is currently coming into existence, a background that will both guide and constitute what counts as ‘thinking’. It begins by providing a capsule history of how this background has become a more and more pervasive quality of Euro-American cultures as a result of the rise of ‘qualculation’. It then moves on to consider how this qualculative background is producing new apprehensions of space and time before ending by considering how new kinds of sensorium may now be becoming possible. In this final section, I illustrate my argument by considering the changing presence of the hand, co-ordinate systems and language, thereby attempting to conjure up the lineaments of a new kind of movement-space.     

毛细管电泳技术分析炸药残留物中无机离子

目的　建立炸药残留物中无机离子的毛细管电泳检测方法。方法　运用缓冲溶液使无机离子在毛细管电泳中达到分离 ,采用紫外间接检测法分析。结果　该方法可从爆炸残留物中准确检测出无机阴离子 (Cl- 、SO2 - 4、NO- 2 、NO- 3、ClO- 3和ClO- 4离子 )和无机阳离子 (NH 4、K 、Na 和Mg2 离子 )。结论　操作方法简便快捷 ,检材用量少 ,检测极限可达ng/ml级 ,分析结果满意     

被复制钥匙痕迹的研究

目的　找出一字型和十字型齿状钥匙被复制后形成的痕迹特征 ,为检案提供参考。方法　取 1 32把一字型齿状钥匙和 4 4把十字型齿状钥匙进行复制并经OLYMPUS连续变倍立体显微镜观察。结果　被复制钥匙的侧面 ,匙槽沟 ,牙花顶部曲面或钥匙凹窝面上均有明显的痕迹。结论　根据被复制钥匙痕迹特征 ,能为检验认定提供主要依据     

健全行政执法检察监督机制的路径探析

"穿透式"行政检察监督是指检察机关从监督法院行政审判活动穿透至监督行政机关作出的行政行为。为进一步推动检察机关做实行政检察工作,做到"一手托两家",促进依法行政,建议从"穿透式"行政检察监督面临的困境、监督的必要性及可行性、监督的范围及对象、监督的方式及手段、监督机制的构建等方面对狭义行政执法检察监督的定位与界限进行探索,从而为推进社会治理现代化进程提供可行的建议。     

论收受财物后退还或者上交的认定

情节显著轻微危害不大是认定收受请托人财物后及时退还或者上交不构成受贿罪的根据。因此，收受请托人财物，是指被动收取财物而非主动索取财物；退还请托人财物必须是主动退还；上交请托人财物必须上交给纪检监察部门指定的账户；如果国家工作人员为请托人谋取不正当利益，即使及时退还或者上交，仍然应当构成受贿罪。同时，廉政账户制度必须纳入司法化的轨道才能获得新生。