H1N1亚型猪流感病毒HA基因的表达及单克隆抗体的制备

采用RT-PCR方法扩增了H1N1亚型猪流感病毒(SIV)广东株的血凝素(HA)基因,测序后对HA基因进行了生物信息学分析。将HA基因克隆到高效可溶表达载体pBCX上,成功构建重组表达质粒pBCX-HA。将其转化到大肠杆菌BL21(DE3)感受态细胞中,用IPTG进行诱导表达。诱导表达的HA融合蛋白分子质量约为94.0 ku,表达产物占菌体总蛋白的29.5%。经Western-blot分析证实可溶表达的融合蛋白与H1N1亚型猪流感病毒阳性血清具有良好的免疫反应性。用纯化的HA融合蛋白作包被抗原用于猪流感病毒单克隆抗体的筛选,成功筛选到2株针对HA蛋白抗原表位的单克隆抗体;ELISA结果表明,制备的SIV单克隆抗体ⅢF6A4C10与H1N1亚型SIV抗原具有特异的免疫反应性,为H1N1 SIV的鉴别诊断奠定了基础。     

教师"三商"刍议--培养创新人才所需的教师素质

培养创新人才需要高素质的教师。高素质的教师具备高智商、高情商、高灵商。在对“三商”内涵进行分析的基础上,具体探讨了为培养创新人才教师所应具备的素质。     

FAMILY COURT IMPROVEMENT AND THE ART OF GRANTSMANSHIP: A JUDGE'S PERSPECTIVE

Many American courts face funding obstacles as they try to create and implement unified family court processes that have not been part of the traditional family court adversarial process. To create new staff positions, build and equip adequate facilities, and create and implement innovative programs, grant funding may be available to supplement what local and state governments provide. While social science research confirms that children are adversely affected by exposure to high‐conflict family litigation and domestic violence, many communities have few affordable and available resources to address these issues. This article presents lessons and principles that one court learned as it applied for a grant to design programs for high‐conflict families and families with domestic violence history.     

高介入度市场品牌资产构成要素对顾客重购意愿的影响研究

研究品牌资产构成要素对顾客重复购买意愿的影响可以发现，不同品牌的资产维度影响顾客重购意愿的方式与路径各不相同。从高介入度市场着手，通过对高介入度产品笔记本和手机的市场调查和相关数据分析，结果发现，在高介入度市场的品牌资产构成要素中，品牌认知、感知价值是直接影响顾客重购意愿的品牌资产关键维度，而品牌形象、感知质量和品牌独特性则通过品牌认知、感知价值间接影响顾客的重购意愿。     

Fatal accidental hanging by a high-chair waist strap in a 2-year-old girl

High chairs are commonly used to feed children after 6 months. Related injuries are oftentime minor and rarely leading to death. We describe a case of a 2-year-old female child who used to jump alone on her high chair and also had the habit to fasten the straps by herself. Her mother found her hanging by the waist straps. A thorough investigation showed that she climbed her high chair and fastened the waist straps but not the crotch one. The girl slid down into the seat, trapping her neck in the waist straps and thus resulting in hanging. In here, we concluded that the victim's death was caused by asphyxia, itself, caused by accidental hanging. The present case is of a special interest because of the rare similar cases reported. This case suggests that a correct restraint use and a close supervision would have prevented such a fatal issue.