口蹄疫病毒3B基因的高效表达及其表达产物的反应活性

通过PCR技术获得口蹄疫病毒非结构蛋白3B基因,将其克隆至质粒pET-32a( )和高效可溶性表达质粒pEM中,经PCR筛选,获得阳性重组子pET-3B和pEM-3B,将它们转化大肠杆菌BL21中进行诱导表达,SDS-PAGE及Western-blot检测结果证明,表达的3B融合蛋白能与FMDV感染动物血清发生特异性反应。蛋白可溶性分析表明,EM-3B融合蛋白绝大部分以可溶形式表达。以此可溶性融合蛋白EM-3B为抗原建立了可区分FMD免疫动物和感染动物的EM-3B-DIVA-ELISA方法,经检测,其特异性为95.0%,敏感性为97.5%,与2个国产试剂盒的符合率分别为96.25%和98.75%。     

证明标准建构的虚幻与现实——从考察两大法系国家的证明标准历史出发

民事诉讼证明标准是衡量法官形成内心确信的尺度.本文通过对证明标准在两大法系国家的统一和分离历史的考察,探讨不同法系确立不同的民事诉讼证明标准的原因,进而在借鉴国外经验的基础上构建我国的民事诉讼证明标准.     

公新世纪我国高职教育的培养目标

培养目标是高职教育的定位依据，树立新世纪我国高职教育的人才观、质量观和教育观，确立我国学历性和非学历性高职教育的目标定位，明确我国高职教育的教学目标和发展目标，是我国高职教育发展面临的首要问题。     

儒家终极关怀与思想政治教育

随着商品经济和科学技术的迅猛发展,人们的精神安顿和终极关怀的问题日益凸显.科学有效的思想政治教育是解决终极关怀问题的重要手段.在中国悠久的文化历史中,儒家形成了一整套终极关怀理论.从儒家终极关怀理论中汲取智慧,联系实际加强和改进思想政治教育,将这两方面有机地结合起来,是解决现代人终极关怀的一条有效途径.     

邓小平经济伦理思想新探

以人为本是邓小平经济伦理思想的核心,和谐发展是邓小平经济伦理思想的精神实质,经济公正是邓小平经济伦理思想的根本原则,人的发展是邓小平经济伦理思想的终极目标。     

培育高新技术原创产业集群的战略选择

高新技术产业集群是一种新的区域经济模式,它是区域创新系统的核心,它的出现和发展提升了区域产业的竞争力,促进了地区经济的快速发展。根据我国不同区域产业集群的特点,我国应首先打造原创产业集群,并从财政税收上加以扶持激励,以促成我国高新技术原创产业集群的形成和发展。     

民事诉讼中判断电子证据真实性的标准

在涉及电子证据的民事案件中 ,由于影响电子证据真实性的因素很多 ,对它们都予以审查是不可能的 ,这时就需要根据电子证据真实性的标准来判断 ,必要时应采取“高度盖然性”标准 ,这是判断证据真实性的最低标准。采用这一标准有助于法官在证据的真实性没有确凿把握的情况下进行裁判 ,避免了犹豫不决而导致的拖延诉讼或拒绝裁判     

论行政许可法对政府的诠释

2003年8月27日,十届全国人大常委会四次会议通过了《中华人民共和国行政许可法》。这部法律的颁布施行,是中国政府顺应市场经济体制发展,适应WTO规则,与世界接轨的客观要求。这部法律对我国政府职能的转变将产生巨大影响,它为我国政府作出了有限政府、服务政府、高效政府、透明政府、诚信政府、责任政府等的崭新诠释。     

An Impact Velocity Device Design for Blood Spatter Pattern Generation with Considerations for High‐Speed Video Analysis,

A mechanical device that uses gravitational and spring compression forces to create spatter patterns of known impact velocities is presented and discussed. The custom‐made device uses either two or four springs (k₁ = 267.8 N/m, k₂ = 535.5 N/m) in parallel to create seventeen reproducible impact velocities between 2.1 and 4.0 m/s. The impactor is held at several known spring extensions using an electromagnet. Trigger inputs to the high‐speed video camera allow the user to control the magnet's release while capturing video footage simultaneously. A polycarbonate base is used to allow for simultaneous monitoring of the side and bottom views of the impact event. Twenty‐four patterns were created across the impact velocity range and analyzed using HemoSpat. Area of origin estimations fell within an acceptable range (ΔX_av = ?5.5 ± 1.9 cm, ΔY_av = ?2.6 ± 2.8 cm, ΔZ_av = +5.5 ± 3.8 cm), supporting distribution analysis for the use in research or bloodstain pattern training. This work provides a framework for those interested in developing a robust impact device.     

Forensic Investigation of Formaldehyde in Illicit Products for Hair Treatment by DAD‐HPLC: A Case Study

The illegal use of formalin (commercial formaldehyde) in cosmetic products harms the health of individuals exposed to this substance. Over the last years, the commercial availability of these products, especially those containing irregular dosage of formaldehyde, has increased in Brazil. This work analyzes some products for hair treatment available in the Brazilian market and verifies their safety. The adopted analytical methodology involved sample derivatization with 2,4‐dinitrophenylhydrazine, followed by high‐performance liquid chromatography with ultraviolet detection (UV–VIS) at λ = 365 nm. The limit of quantification is 2.5 × 10^?3% w/w, and the recovery tests were around 93%. Some of the samples contained high and illegal formaldehyde levels ranging from 9% to 19% (w/w) and others presented suitable concentrations of the analyte. On the basis of the results, this work discusses the efficiency and practicality of this analytical method for forensic purposes.