地面三维激光扫描在交通事故勘查及重建中的应用进展

本文回顾了地面三维激光扫描技术的发展历程和应用场景,简要阐述了地面三维激光扫描系统的技术原理和作业流程,对地面三维激光扫描技术在交通事故领域的应用进行分类,并详细梳理了各类别下的最新研究成果与实例,结合交通事故勘查和重建发展需求,探讨了目前存在的问题和未来发展方向。     

沙冬青生物碱对MDCC-MSB1细胞增殖抑制与诱导凋亡的影响

采用体外细胞培养技术、噻唑蓝(MTT)还原法、透射电子显微镜观察及流式细胞技术检测梯度浓度沙冬青总生物碱对体外培养鸡马立克氏病肿瘤HDCC-MSB1细胞的增殖抑制及诱导凋亡的作用。结果显示,沙冬青生物碱可显著抑制HDCC-MSB1细胞增殖,且这种抑制具有剂量和时间效应关系。经沙冬青生物碱作用的HDCC-MSB1细胞培养样本中有明显的DNA低含量颗粒(亚G1期峰),细胞周期各时相分布发生改变,细胞增殖在G1被阻滞,并进一步诱导HDCC-MSB1细胞凋亡。电子显微镜观察也表明,经沙冬青生物碱作用的HDCC-MSB1细胞培养样本中,大量HDCC-MSB1细胞凋亡。结果表明,沙冬青生物碱对HDCC-MSB1细胞增殖有显著抑制效应,其抑制机理为诱导HDCC-MSB1细胞凋亡。     

MALDI-TOF-MS对DAI大鼠脑干蛋白质组学的分析

目的应用基质辅助激光解吸/电离-飞行时间质谱(matrix-assisted laser desorption/ionization timeof-flight mass spectrometry,MALDI-TOF-MS)技术建立弥漫性轴索损伤(diffuse axonal injury,DAI)诊断模型,筛选DAI相关蛋白或多肽,为寻找DAI的分子标志物提供理论基础。方法将15只雄性SD大鼠随机分为DAI组(n=10)和对照组(n=5),采用MALDI-TOF-MS技术对两组大鼠脑干组织的蛋白或多肽表达谱进行检测,运用Clin Pro Tools 2.2分析软件筛选出具有差异的分子峰,应用遗传算法建立诊断模型。结果筛选出有差异的分子峰61个(P0.05),其中DAI组有9个分子峰下调,52个分子峰上调。筛选出5个分子峰建立诊断模型,交叉验证的特异性为96.14%、敏感性为95.98%,盲法验证结果的特异性为73.33%、敏感性为70.00%。结论应用MALDI-TOF-MS技术不仅能够建立特异性和敏感性较高的DAI诊断模型,还可以筛选差异蛋白或多肽,为DAI的法医学鉴定奠定理论基础。     

坚定理想信念 践行群众路线

作为党的领导干部我们要坚定理想信念,主要任务是建设高素质的领导干部队伍,我们可以定期对党员领导干部进行教育培训。开展群众路线主题教育实践活动,是在新时期全党开展的又一次教育性活动,主要目的在于让全党同志谨记全心全意为人民服务思想,也就是说,我们广大党员要用理想信念的强大力量激发起我们对践行群众路线的真情感、真认同、真落实,做有远大理想、宽广胸怀的共产党人。     

一次手印鉴定引发的思考

近期,我们受理了一起特殊的手印鉴定。此前该手印已有肯定与否定两种截然相反的鉴定结论,鉴定委托人又不能提供检材和样本原件,只将相关的照片送检。在检验过程中,我们经过观察分析、实验验证、计算机图像处理等过程,弄清了手印的本质,做出了科学、客观的结论。     

论网上侦查

网上侦查,是以计算机网络为支撑,以刑事犯罪情报信息及相关管理信息为核心开展的侦查破案方式。网上侦查为侦查破案提供了大量可查信息,可以确保侦查部门的快速反应,实现侦查协作,丰富侦查破案模式,提高侦查破案的效率。网上侦查具有快速应答、批量比对、结果相对客观、过程保密、远程操作、侦查成本低等特点。网上侦查能够有效地打击系列犯罪、流窜犯罪、重新犯罪和多发性犯罪。发展和深化网上侦查,必将大大提高侦查破案效率。     

铁路线路爆炸案件现场勘验研究

铁路线路爆炸是反映铁路线路爆炸发生原因、过程和结果的物质基础。铁路线路爆炸案件现场特殊性是由铁路的行业性、爆炸的特性和铁路在社会交通中的重要地位所决定的。铁路线路爆炸现场的特点 :1.现场存留从属于线路开通 ;2 .炸点通常为炸坑、轨断或轨变 ;3.线—车现场构成的特殊性 (车底爆炸形成下压 ) ;4 .爆炸物证受到地形地貌影响突出 ;5 .现场位置常为线路偏僻要害处。铁路线路爆炸现场勘验的重点 :1.判定炸点 ;2 .推断爆炸装置 ;3.估算炸药重量 ;4 .确定爆炸性质 ;5 .分析现场环境格局 ;6 .判定犯罪嫌疑人。     

论党的思想路线与理论创新

纵观中国共产党的历史,坚持党的思想路线和理论创新,两者之间有着密切的联系。可以说,党的思想路线与党的理论创新是有机结合、相辅相成的。中国共产党的思想路线是贯穿于党的历史的思想红线,坚持党的思想路线,就必然要不断推进党的理论创新,形成理论创新的成果。坚持党的思想路线需要进行理论创新,理论创新是坚持党的思想路线的内在要求。党的理论创新要坚持理论联系实际和深入有效的调查研究方法,坚持党的民主集中制和实践是检验理论创新成果的重要标准。     

Characterization of Printing Inks Using DART‐Q‐TOF‐MS and Attenuated Total Reflectance (ATR) FTIR

The rise in improved and widely accessible printing technology has resulted in an interest to develop rapid and minimally destructive chemical analytical techniques that can characterize printing inks for forensic document analysis. Chemical characterization of printing inks allows for both discrimination of inks originating from different sources and the association of inks originating from the same source. Direct analysis in real‐time mass spectrometry (DART‐MS) and attenuated total reflectance Fourier transform infrared spectroscopy (ATR‐FTIR) were used in tandem to analyze four different classes of printing inks: inkjets, toners, offset, and intaglio. A total of 319 samples or ~ 80 samples from each class were analyzed directly on a paper substrate using the two methods. DART‐MS was found to characterize the semi‐volatile polymeric vehicle components, while ATR‐FTIR provided chemical information associated with the bulk components of these inks. Complimentary data results in improved discrimination when both techniques are used in succession resulting in >96% discrimination for all toners, 95% for all inkjets, >92% for all offset, and >54% for all intaglio inks.     

Evaluation of the Forensic Utility of Scanning Electron Microscopy‐Energy Dispersive Spectroscopy and Laser Ablation‐Inductively Coupled Plasma‐Mass Spectrometry for Printing Ink Examinations

Improvements in printing technology have exacerbated the problem of document counterfeiting, prompting the need for analytical techniques that better characterize inks for forensic analysis and comparisons. In this study, 319 printing inks (toner, inkjet, offset, and Intaglio) were analyzed directly on the paper substrate using scanning electron microscopy‐energy dispersive spectroscopy (SEM‐EDS) and Laser Ablation‐Inductively Coupled Plasma‐Mass Spectrometry (LA‐ICP‐MS). As anticipated, the high sensitivity of LA‐ICP‐MS pairwise comparisons resulted in excellent discrimination (average of ~ 99.6%) between different ink samples from each of the four ink types and almost 100% correct associations between ink samples known to originate from the same source. SEM‐EDS analysis also resulted in very good discrimination for different toner and intaglio inks (>97%) and 100% correct association for samples from the same source. SEM‐EDS provided complementary information to LA‐ICP‐MS for certain ink types but showed limited utility for the discrimination of inkjet and offset inks.