DVI-300微痕迹色差分析系统对激光打印文字与印章印文交叉时序检验的方法研究

目的研究常见的打印字迹与不同印章印文交叉时序的相关性,为检验提供依据。方法应用DVI-300微痕迹色差分析系统对制成的实验样本进行实体观察,并对微痕迹色差模型图进行特征分析。结果先墨后朱,交叉部位字迹边缘状态完整,等高线宽度、流向未见明显变化;先朱后墨,交叉部位中心字迹等高线排列出现游离状态,色料分布发生数字图形曲面变化。结论用本系统可以观察、分析朱墨交叉部位的微观特征和无损检验研究。     

激光捕获显微分离技术在DNA检验中的应用研究

目的建立一套显微细胞捕获技术用于法医学生物检材DNA检验方法。方法使用VeritasTM LCM激光捕获仪,采用紫外加红外的捕获方式,对框架覆膜玻片上经苏木素染色口腔上皮细胞进行捕获,采用改良硅珠法提取细胞DNA,使用Identifiler TM试剂盒在5μL体系中进行PCR扩增。结果成功获得20个口腔上皮细胞的13个以上完整STR基因座分型谱带。结论本研究建立的方法适合法医学生物检材制成的染色涂片上细胞的DNA检验。     

Laser desorption/ionization time-of-flight mass spectrometry of triacylglycerols and other components in fingermark samples

The chemical composition of fingermarks could potentially be important for determining investigative leads, placing individuals at the time of a crime, and has applications as biomarkers of disease. Fingermark samples containing triacylglycerols (TAGs) and other components were analyzed using laser desorption/ionization (LDI) time-of-flight mass spectrometry (TOF MS). Only LDI appeared to be useful for this application while conventional matrix-assisted LDI-TOF MS was not. Tandem MS was used to identify/confirm selected TAGs. A limited gender comparison, based on a simple t-distribution and peaks intensities, indicated that two TAGs showed gender specificity at the 95% confidence level and two others at 97.5% confidence. Because gender-related TAGs differences were most often close to the standard deviation of the measurements, the majority of the TAGs showed no gender specificity. Thus, LDI-TOF MS is not a reliable indicator of gender based on fingermark analysis. Cosmetic ingredients present in some samples were identified.     

激光捕获显微切割技术用于分离混合斑中精子细胞

目的 评估激光捕获显微切割(laser capture microdissection.LCM)技术在分离混合斑中少量精子细胞的应用价值.方法 配制不同比例的精液-阴道上皮细胞混合液.分别用差异裂解法和LCM法分离精子细胞,用磁珠法提取精子细胞DNA,并用IdentifilerTM试剂盒进行STR基因型检测.结果 LC...     

The detection of multiply charged dyes using matrix-assisted laser desorption/ionization mass spectrometry for the forensic examination of pen ink dyes directly from paper

Abstract:  Laser desorption mass spectrometry (LDMS) is emerging as a technique for questioned document examination. Its use is limited to detecting ink dyes that are neutral or singly charged. Several inks contain dyes that are multiply charged and LDMS cannot be employed for their identification. We have successfully detected >20 polyionic dyes that can be used in the manufacture of inks using matrix-assisted laser desorption/ionization (MALDI) MS, directly from paper, with the matrix, 2-(4-hydroxyphenylazo)benzoic acid (HABA), and the additive, diammonium hydrogen citrate (DAHC). For example, Acid Violet 49, a charged dye containing one positively-charged site and two negatively charged sulfonate groups, cannot be detected by LDMS, but forms intact, singly charged ions in the MALDI MS experiment. The method described is also useful for identifying multiply charged dye mixtures that are used in modern pen inks.     

论小额速裁程序的优化与完善

201 3年新民诉法推出了小额速裁程序,然而可供司法实践适用的规定匮乏。小额速裁程序实行一审终审,当以司法效率为重,保证底线正义。在司法运行中,小额速裁程序存在着适用条件不完善、启动机制不明确、审理程序不简化及救济程序缺失等问题,应当从严限定小额速裁程序的适用范围,法院享有启动权,简化和优化审判程序,设置判决异议程序,疏导当事人救济诉求,尽快健全与完善小额速裁程序。     

邓小平领导开创中国特色社会主义道路的认识路线和思想方法——兼论中国道路的精髓和真谛

邓小平的历史性贡献是多方面的,其中最伟大的贡献,莫过于领导党和人民在改革开放中创造性地开拓了中国特色社会主义道路。邓小平领导开创中国特色社会主义道路的认识路线和思想方法突出体现在:把实践作为逻辑起点,在坚持马克思主义基本原理同中国具体实际相结合中开创中国特色社会主义道路;把规律作为根本依据,在探索社会主义初级阶段改革与发展基本规律中开创中国特色社会主义道路;把人民作为主体力量,在实现人民群众利益需求和创造精神有机统一中开创中国特色社会主义道路。中国特色社会主义道路来之不易,必须深刻把握这条道路的精髓和真谛,坚定不移沿着这条道路开拓前进。     

Identification of colorants in pigmented pen inks by laser desorption mass spectrometry

Pigments are rapidly replacing dyes as colorants in pen and printer inks, due to their superior colors and stability. Unfortunately, tools commonly used in questioned document examination for analyzing pen inks, such as TLC, cannot be used for the analysis of insoluble pigments on paper. Laser desorption mass spectrometry is demonstrated here as a tool for analyzing pigment-based pen inks. A pulsed nitrogen laser can be focused onto a pen stroke from a pigmented ink pen on paper, and positive and negative ions representative of the pigment can be generated for subsequent mass spectrometric analysis. Targeted pens for this work were a set of Uni-ball 207 pigmented ink pens containing blue, light blue, orange, green, violet, red, pink, and black inks. Copper phthalocyanine was identified as the pigment used to make both blue inks. A mixture of halogenated copper phthalocyanines were identified in the green ink. Unexpectedly, the pink ink was found to contain a red pigment, Pigment Red 12, treated with a mixture of water-soluble dyes. Each sample yielded ions representative of the pigments present.     

群众路线是党的生命线

群众路线是我党的生命线 ,是马克思主义历史唯物主义世界观和科学方法论的体现 ,只有始终不渝地坚持群众路线 ,才能成为广大人民群众根本利益的忠实代表。