In search of blood--detection of minute particles using spectroscopic methods

An examination protocol for rapid detection of remnants of blood particles on garments of suspects in bloody murder cases is described. Microparticles of blood are sampled along with fibres and hairs using the tape lifting method. The tapings are searched with a low power microscope for red particles with morphology similar to blood. Presumed blood traces are further examined using microspectrophotometry on the cut out piece of taping. The typical visible spectrum of haemoglobin is characteristic for blood. Alternatively Raman spectroscopy can be used to measure the characteristic vibrational spectrum of haemoglobin. At a later stage, these particles may be removed from the piece of taping in order to extract the blood and attempt to obtain a genetic profile.     

显微红外光谱仪与显微拉曼光谱仪对纺用无色单根纤维的检测

目的研究常见纺用单根无色纤维的有效鉴别方法。方法使用显微红外光谱仪、显微激光拉曼光谱仪研究7大类纺用单根无色纤维的分子光谱。结果显微红外光谱仪、显微激光拉曼光谱仪能有效区分包括棉、粘胶、羊毛、丝、聚酰胺、聚丙烯腈和聚酯在内的7种纤维,是检测单根纤维的有效手段。785nm激发光源是显微激光拉曼光谱仪研究这7类纤维的最佳波长。但由于纺用纤维生产过程的标准化,仅依据红外或者拉曼的峰位置区分同种类、不同产地纤维的效果一般。结论显微红外光谱仪、显微激光拉曼光谱仪是鉴别常见纺用单根无色纤维的有效方法。     

拉曼光谱法鉴别常见炸药

目的建立一种识别炸药的方法。方法使用激光显微拉曼光谱法对17种常见炸药粉末进行了分析。结果17种常见炸药均有丰富的拉曼位移峰,可以通过其位移峰峰位的不同区分不同成分的炸药。结论拉曼光谱法适用于快速准确地识别炸药。     

Preliminary Study on Diffuse Axonal Injury by Fourier Transform Infrared Spectroscopy Histopathology Imaging

The objective of this study was to evaluate the application of Fourier transform infrared (FTIR) spectroscopy for detecting diffuse axonal injury (DAI) in a mouse model. Brain tissues from DAI mouse model were prepared with H&E, silver, and β‐amyloid precursor protein (β–APP) immunohistochemistry stains and were also studied with FTIR. The infrared spectrum images showed high absorption of amide II in the subcortical white matter of the experimental mouse brain, while there was no obvious expression of amide II in the control mouse brain. The areas with high absorption of amide II were in the same distribution as the DAI region confirmed by the silver and β‐APP studies. The result suggests that high absorption of amide II correlates with axonal injury. The use of FTIR imaging allows the biochemical changes associated with DAI pathologies to be detected in the tissues, thus providing an important adjunct method to the current conventional pathological diagnostic techniques.     

精神视界的开发与“点”的深入——谈“中国当代文学”的教学

本文探讨大学中文专业基础课＂中国当代文学＂教学中的精神追求与方法特征。文章强调挖掘中国当代文学发展历程所蕴涵着的丰富精神内涵、突出它与源远流长的世界文学和中国古代文学之间的深度关联,并阐述了教学过程中的＂问题意识＂和情感体验在文学教育中的重要性,反对平面化单一性的知识介绍。     

论艾略特的传统观与非个性化批评理论

艾略特是20世纪活跃在世界文坛上的伟大作家、诗人和重要的文学批评家。他所倡导的传统观和非个性化的批评理论开创了文学批评的一个崭新时代。本文从艾略特对17世纪两位著名诗人约翰·邓恩和约翰·弥尔顿所持的截然不同的观点着手,从正反两个方面诠释他的批评理论,即在对待传统与创新的问题上提倡的是一种有古有今、传统与创新共存的文学观。     

近红外光谱法快速分析玄参药材中毛蕊花糖苷含量

目的 建立玄参药材指标成分毛蕊花糖苷的近红外光谱快速定量模型。方法 采用偏最小二乘法建立玄参药材中毛蕊花糖苷的近红外光谱多元校正模型,并对未知样品进行含量预测。结果 校正模型相关系数r＝0.998 53,验证集预测误差平方根为0.027 3,验证集平均相对误差为3.7%。结论 近红外光谱检测快速,模型预测效果好,可用于玄参药材中毛蕊花糖苷的快速测定。     

DNA数据库建设中批量样本直接扩增检验法的应用

目的研究批量样本直接扩增法在DNA数据库建设中的应用。方法打孔机打取血滤纸600份,剪取芝麻大小的口腔拭子300份,放入96孔扩增板,加入扩增试剂后直接扩增,并对其中200份血滤纸用磁珠法,100份口腔拭子用96孔板Chelex-100法,经DNA提取后扩增检验进行比较。结果血滤纸采用直接扩增法及磁珠法STR检测成功率均为100%;口腔拭子采用直接扩增法的成功率为95%,采用96孔板Chelex-100法的成功率为94%。结论血滤纸和口腔拭子通过直接扩增均能获得很好的DNA分型结果,该方法省时省力,可用于DNA数据库建设。     

Magnetic Fingerprint Powder from a Mineral Indigenous to Thailand

Abstract: A study was conducted to investigate whether natural magnetite (Fe₃O₄), which is an abundant mineral in Thailand, could be used as a magnetic powder in the detection of latent fingerprints. Because of the presence of impurities, powdered magnetite is only weakly attracted by a magnet and cannot be used as a magnetic fingerprint powder by itself. Mixing a small amount of magnetite powder with nickel powder greatly enhances the magnetic attraction. A mixture of magnetite powder and nickel powder in a mass ratio of approximately 1:100 was found to be suitable for use as a magnetic fingerprint powder. Fingerprints developed using the magnetite/nickel mixture on nonporous surfaces were found to exhibit good adherence and clarity. Using an automated fingerprint identification system, the number of minutiae detected in fingerprints developed by using the prepared powder on nonporous surfaces was found to be comparable to those detected in fingerprints developed by using a commercial black magnetic powder. The cost is lowered by more than 60%.     

高校图书馆图书磁条转换工作的实践与思考——以湖北第二师范学院图书馆为例

本文首先论述了高校图书馆顺应“大流通”服务模式的潮流进行图书磁条转换工作的必要性和可行性,然后重点介绍了图书馆适应招投标法有条不紊地组织好该项工作的具体过程,最后强调了做好该项工作需要注意的有关事项。