广泛软组织挫伤--挤压综合征

本文通过回顾国内外医学文献,对广泛性软组织挫伤--挤压综合征的研究现状和取得的成就进行了介绍,并对其今后的发展趋势进行了探讨.     

猪生殖与呼吸综合征病毒宁夏分离株ORF5基因的克隆表达及其表达产物的活性分析

参考已发表的猪生殖与呼吸综合征病毒(PRRSV)ORF5基因序列,设计合成了1对覆盖完整ORF5基因区段的引物,克隆了PRRSV NX/HY株的ORF5基因(登录号为EU600287),并进行了序列比较.将该基因克隆到原核表达栽体pGEX-6P-1中,构建了融合表达质粒pGEX-6P-1-5并转化BL21,对表达蛋白进行Western-blot分析.结果表明,NX/HY分离株ORF5基因与PRRSV JX1、CH-la株的核苷酸序列同源性分别为99.2%和95.2%,推导的氨基酸序列的同源性分别为98.5%和92.5%;层析扫描分析显示,目的蛋白的含量约占菌体总蛋白的30%,且具有良好的反应原性.GP5蛋白的表达为建立相应的血清学诊断方法奠定了基础.     

五种重要猪病病毒基因芯片探针的建立及其应用

将猪繁殖与呼吸综合征病毒(PRRSV)、猪瘟病毒(CSFV)、猪细小病毒(PPV)、猪伪狂犬病痛毒(PRV)、猪圆环病毒2型(PCV-2)的特异cDNA片段作为探针点制于氨基修饰的玻片上,以这5种细胞毒的核酸作为模板,进行不对称PCR扩增,制备靶物,经绿色荧光染料Cy3标记后,分别与cDNA微阵列的芯片探针进行杂交,杂交信号经Genepix 4000A扫描仪扫描.结果显示,该芯片探针可以特异地与Cy3标记的这5种靶物结合.用该基因芯片对100份现地采集的猪全血样品进行检测,检出PRRSV阳性样品26份,PCV-2阳性样品47份,PRRSV和PCV-2混合感染阳性样品17份,PPV阳性样品5份,PRV阳性样品2份,CSFV阳性样品20份.表明,本试验研制的cDNA芯片探针可以特异地检测这5种猪病病毒,具有良好的诊断应用前景.     

Finding of Hyperostosis Frontalis Interna During the Autopsy Procedure: Forensic Issues

Hyperostosis frontalis interna is a common phenomenon, which may have been overrated in its significance in the past, and may, currently be underrated in its significance. We present three cases of hyperostosis frontalis interna found during medicolegal autopsies and discuss their forensic considerations. The patients were all middle‐aged women with metabolic and endocrine manifestations and psychiatric ailments; thickening of the inner table of the frontal bone of the skull was found during each autopsy. We describe the relationship between hyperostosis frontalis interna, metabolic manifestations, and neuropsychiatric symptoms as part of Morgagni‐Stewart‐Morel syndrome. There is still considerable disagreement in the scientific community as to whether this syndrome is a clinical entity. Nonetheless, awareness of Morgagni‐Stewart‐Morel syndrome can be of help in understanding the circumstances surrounding death. In some other cases, hyperostosis frontalis interna could be used by forensic pathologists as criteria for sexing and aging a skeleton.     

Repeat Coronary Artery Dissection in Pregnancy: A Case Report and Review of the Literature

Non‐atherosclerotic spontaneous coronary artery dissection (NA‐SCAD) is a rare cause of morbidity and mortality with a propensity for young, healthy, and often peripartum women. NA‐SCAD etiology is poorly understood, with possible hormonal and hereditary mechanisms. Current treatment strategies range from conservative management (often showing resolution on angiographic follow‐up) to invasive angiographic procedures. Rarely, NA‐SCAD has recurred in another coronary artery, ranging hours to years later. We report NA‐SCAD of the right coronary artery (RCA) in a 30‐year old, 3‐month postpartum female with an additional autopsy finding of remote myocardial infarction (MI) in the left anterior descending (LAD) coronary artery territory. The remote MI is consistent with prior NA‐SCAD of the LAD and, given the medical history, may have occurred in the peripartum period of the decedent first pregnancy 3 years earlier. As such, to the best of our knowledge, this may represent the first reported case of NA‐SCAD recurrence in a subsequent pregnancy.     

Fingerprint Change: Not Visible,But Tangible

Hand–foot syndrome, a chemotherapy‐induced cutaneous toxicity, can cause an alteration in fingerprints causing a setback for cancer patients due to the occurrence of false rejections. A colon cancer patient was fingerprinted after not having been able to use fingerprint recognition devices after 6 months of adjuvant chemotherapy. The fingerprint images were digitally processed to improve fingerprint definition without altering the papillary design. No evidence of skin toxicity was present. Two months later, the situation returned to normal. The fingerprint evaluation conducted on 15 identification points highlighted the quantitative and qualitative fingerprint alteration details detected after the end of chemotherapy and 2 months later. Fingerprint alteration during chemotherapy has been reported, but to our knowledge, this particular case is the first ever reported without evident clinical signs. Alternative fingerprint identification methods as well as improved biometric identification systems are needed in case of unexpected situations.     

温病湿热证湿重于热动物模型舌的病理观察

目的：观察温病湿热证湿重于热动物模型舌的病理改变。方法：复制温病湿热证湿重于热动物模型,观察大鼠舌的病理改变,并设湿热组、脾虚组、正常组进行比较。结果：湿重于热组、湿热组固有层乳头密度及核分裂相频数显著增多,与正常组及脾虚组比较差异有显著性;湿重于热组和湿热组固有层均出现炎症、充血及水肿,前者水肿更甚,后者则充血明显。结论：舌的病理改变,可能是湿热证型中舌苔变化的病理基础,并可作为判定湿热轻重的指标之一。     

Fatal acute intracranial injury, subdural hematoma, and retinal hemorrhages caused by stairway fall

We describe an infant with an acute subdural hematoma, a fatal head injury, and severe hemorrhagic retinopathy caused by a stairway fall. His cerebral and ocular findings are considered diagnostic of abusive head trauma by many authors. Our literature search of serious injuries or fatalities from stairway or low-height falls involving young children yielded 19 articles of primary data. These articles are discrepant, making the classification of a young child's death following a reported short fall problematic. This case report contradicts the prevalent belief of many physicians dealing with suspected child abuse that low-height falls by young children are without exception benign occurrences and cannot cause fatal intracranial injuries and severe retinal hemorrhages. The irreparable harm to a caregiver facing an erroneous allegation of child abuse requires physicians to thoroughly investigate and correctly classify pediatric accidental head injuries.     

Sickle cell trait-associated deaths: a case series with a review of the literature

This study presents a series of 16 carriers of hemoglobin S (HbS) who died during various circumstances. Many of the cases were associated with mild to moderate exertion. The onset and/or duration of symptoms varied from a few minutes to several hours with many displaying a prolonged lucid interval with stable vital signs. Despite seeking medical treatment, sickle cell trait-related micro-occlusive crisis was never considered in the differential diagnosis. Several cases were associated with sudden death. In those deaths which were delayed, high anion gap and uncompensated metabolic acidosis were typical and were not heat related. Also characteristic were large increases in creatine kinase, alanine aminotransferase, and aspartate aminotransferase along with myoglobinemia. Although the antemortem diagnosis of rhabdomyolysis was made, the underlying cause was never deduced by the clinicians. The sickling found at autopsy is not always a postmortem artifact, and in the right circumstances can be diagnostic.     

原因不明性不孕症中医证候分析

目的 探讨原因不明性不孕症的中医证候特点,为中药治疗原因不明性不孕症提供辨证依据.方法 根据不孕症诊断标准,将219例不孕症患者分为研究组(原因不明性不孕症)108例和对照组(配偶不育症导致的不孕症)111例,分析并比较两组中医证候分布特征,采用二项分类Logistic回归分析原因不明性不孕症的危险证候,以及中医证候与...