wizard clean up在混合斑DNA检验中的应用

目的建立一种快速、简单、有效的混合斑DNA检验方法。方法在100例检案中,利用wizardcleanup直接对精子消化液进行纯化浓缩。采用profilerplus试剂盒进行复合扩增,产物经ABI310基因分析仪检测。结果从混合斑中成功获得精斑10个STR位点的DNA分型。结论wizardcleanup处理混合斑,能有效去除女性成份,得到精斑DNA进行分型。     

The survival and termination of party mergers in Europe

Why do constituent parties that participated in a party merger that was intended to be permanent decide to leave the merger to re‐enter party competition separately? To address this question, merger termination is conceptualised in this article as an instance of new party formation, coalition termination and institutionalisation failure. Building on this conceptualisation, three sets of factors are presented that account for which mergers are likely to be terminated by constituent parties and which are not. To test these three sets of hypotheses, a mixed‐methods design is used. First, survival analysis is applied to a new dataset on the performance of mergers in 21 European democracies during the postwar period. The findings support hypotheses derived from a conception of merger termination as new party formation: pre‐ and post‐merger legislative performance significantly affect the probability of merger termination. Furthermore, the institutionalisation of constituent parties helps to sustain mergers if the latter already built trust in pre‐merger cooperation, in line with the conception of merger termination as institutionalisation failure. Two theory‐confirming case studies are then analysed: one case of merger survival and the other of termination. These case studies substantiate the working of the significant variables identified in the large‐N analysis that drove the selection of case studies. They also reveal how mediating factors difficult to capture in large‐N designs help to account for why factors that – theoretically – should have complicated the working of the ‘survival case’, and should have been beneficial to the ‘termination case’, did not generate the expected effects.     

运用二代测序技术拆分混合STR分型

目的探讨二代测序技术在混合STR分型拆分中的应用。方法对一例强制猥亵妇女案中受害人颈部的拭子和血样作DNA提取,以Precision ID GlobalFiler^TM NGS STR Panel v2试剂盒制备文库,经Ion S5测序仪测序,运用Torrent_Suite_v5.2.1软件进行数据分析后,将检测基因座的序列多态STR分型与长度多态STR分型进行比较。结果在D8S1179、D21S11、D2S441、D2S1338、D10S1248五个基因座发现存在序列特异的等位基因亚型,利用这些亚型对混合STR分型进行了成功拆分。结论二代测序技术提供的等位基因序列信息可对混合STR分型的拆分起到帮助作用。     

Paradoxes of Regionalism and Democracy: Brexit’s Lessons for the Commonwealth

Abstract

On 23 June 2016, 52% of Britons voted in a referendum to exit (Brexit) the European Union (EU) while 48% opted to remain. This is a watershed moment in world politics, given the implications for the future of the UK, the EU and the rest of the world. This paper uses the case of Brexit to analyse paradoxes of regionalism and democracy. The central question is, what does the case of Brexit suggests about the contradictions of democracy and regionalism in the 21st century? Importantly, what broad lessons may be gleaned from the case for regionalist projects among Commonwealth countries?     

Belarusians between East and West

Belarus has a divided identity that reflects its complex history and culture. A mixed-methods investigation incorporating focus groups and national representative surveys conducted over a decade or more suggests that Belarusians themselves are more likely to regard themselves as “European” than their counterparts in Ukraine and Russia, but less likely to do so than in other European countries. There is substantial support for a hypothetical European Union membership, particularly among younger respondents, but there is also strong and widely distributed support for a closer association with the other members of the Commonwealth of Independent States. Consistently, it is the “multidirectional” foreign policy promoted by the current leadership, which seeks closer relations with East and West at the same time, that finds the greatest support. But a “Slavic choice” is also popular, and much more so than a “Western choice” or isolationism.     

A Social Movement Theory Typology of Militant Organisations: Contextualising Terrorism

Typologies are ubiquitous in terrorism studies, illustrating their continued appeal as a tool to further our understanding of this form of political violence. Despite this, to date, the promise of an empirically derived typology has largely been neglected. In addressing this gap, this article sets out a typology developed from Social Movement Theory. Using a novel statistical technique to derive a three-dimensional framework for categorising militant groups, the typology incorporates both organisational characteristics and the wider political context. The result is a typology defined by three conceptual constructs: political capacity, war-making capacity, and network capacity. Alongside these organisational features, imposing measures of the wider political opportunity structure reveals eight types of militant organisation. To explore the utility of the framework, a preliminary analysis interprets the typology in light of the presence of wider conflict. That a robust relationship is found between the various types and whether groups were operating in peacetime, civil war, or low-intensity conflict, goes some way to demonstrating its utility as an analytical tool. Conclusions draw attention to the importance of contextualising militant groups in their socio-political setting, and the benefits of combining theory alongside empirical analysis to develop robust characterisations of violent organisations.     

The Importance of Improving Implementation Research for Successful Interventions and Adaptations

This special issue explores the theoretical underpinnings, triumphs, and challenges of implementing four early childhood education interventions. In doing so, each article highlights the importance of studying the implementation context as part of the evaluation process. This commentary reflects on the entire issue, ultimately arguing that future evaluations must continue to conduct—and improve on—implementation research. Specifically, to understand evaluation findings and scale up or adapt interventions effectively, researchers must examine implementation processes systematically, using both quantitative and qualitative methods. This includes: explaining how interventions were designed, theorizing the relationships between implementation processes and outcomes, defining the implementation phase under study, examining the validity and reliability of implementation measures, and using accessible language in reports.     

Sexual Harassment in the Norwegian Theatre World

ABSTRACT

The aim of this article is to provide insight into the phenomenon of sexual harassment in the theatre world. A survey among Norwegian actors is presented showing that sexual harassment is much more prevalent in the theatre world than in Norwegian work life in general. Further, the article aims at understanding why the prevalence is as high as it is. Based on qualitative interviews, the article points out some risk factors that shed light on the high prevalence of sexual harassment. Lastly, the risk factors are related to charismatic authority as an important power base in the theatre world.     

Choosing your own Boss: Variations of Representation Foci in Mixed Electoral Systems

Members of parliament are privileged agents. They can choose who to regard as their principal: the entire nation, a particular electoral district or a political party. Focusing on two countries with mixed electoral systems, Romania and Hungary, the article documents the dominance of the electoral logic of role-formation over the constraints of legislative organisation and the influence of socialisation. The focus of representation is found to be only modestly influenced by the degree of the embeddedness of MPs in political structures and hierarchies. The association of the seat with a particular territorial unit, on the other hand, has a robust effect even when this association originates in the political environment and not in formal rules. Finally, the psychological effect of losing a Single Member District is also found to influence how MPs perceive their representative role.     

Durable peace and sustainable development in Africa

Zimbabwean migration into South Africa represents a challenge to standard global models for responding to large-scale migration flows. South Africa's existing legal and institutional infrastructure for migration management and refugee protection is not adequate for responding to this challenge and yet the country has not planned or implemented alternative responses. The paper outlines the complex ‘mixed’ nature of current Zimbabwean migration to South Africa and describes how South Africa's range of legal and institutional responses are ineffective in protecting both South Africa's national interests and the rights of Zimbabwean migrants. After presenting a series of factors against which future responses should be evaluated, it lists various possible forms of response, including legal and humanitarian interventions, and discusses their respective appropriateness in the South African context. Finally, the paper suggests six possible policy scenarios, taking into account institutional and political realities on the ground in South Africa.