批量陈旧血样DNA的自动化检验

目的建立批量陈旧血样DNA自动化提取检验的方法。方法采用普通磁珠法和本文建立的磁珠法经TECAN Freedom EVO150—8型自动化工作站分别提取540份陈旧血样模板DNA,采用Sinofiler^TM试剂盒进行荧光标记复合扩增。结果采用普通磁珠法和本文所建DNA提取方法,在540份样本中获得全部基因座STR分型的样本分别为217份和488份,检验成功率分别为40．2％和90．3％。结论本文所建方法可显著提高大批量陈旧血样自动化检验成功率。     

军舰护航打击索马里海盗：法律依据和司法程序安排

军舰护航涉及国内法和国际法双重依据问题,其中联合国安理会的相关决议和索马里政府的同意,构成军舰护航打击索马里海盗的国际法依据。随着打击海盗行动的深入发展,法律合作成为打击海盗国际合作中亟待加强的重要领域。各国应尽快订立或完善刑事罪名,确立对海盗的普遍管辖权;协议海盗疑犯的移交、审判和监管安排,为军舰护航打击海盗提供法律基础;加强对索马里海域沿岸国的援助,帮助其加强国内司法能力建设。     

激光捕获显微切割技术用于分离混合斑中精子细胞

目的 评估激光捕获显微切割(laser capture microdissection.LCM)技术在分离混合斑中少量精子细胞的应用价值.方法 配制不同比例的精液-阴道上皮细胞混合液.分别用差异裂解法和LCM法分离精子细胞,用磁珠法提取精子细胞DNA,并用IdentifilerTM试剂盒进行STR基因型检测.结果 LC...     

Chemical enhancement of footwear impressions in blood on fabric – Part 1: Protein stains

A range of protein stains were utilised for the enhancement of footwear impressions on a variety of fabric types of different colours with blood as a contaminant. A semi-automated stamping device was used to deliver test impressions at a set force to minimise the variability between impressions; multiple impressions were produced and enhanced by each reagent to determine the repeatability of the enhancement. Results indicated that while most protein stains used in this study successfully enhanced impressions in blood on light coloured fabrics, background staining caused interference on natural fabrics. Enhancement on dark coloured fabrics was only achieved using fluorescent protein stains, as non-fluorescent protein stains provided poor contrast.A further comparison was performed with commercially available protein staining solutions and solutions prepared within the laboratory from the appropriate chemicals. Both solutions performed equally well, though it is recommended to use freshly prepared solutions whenever possible.     

Application of mtDNA SNP analysis in forensic casework

In this study six forensic cases are presented where the routine analysis of samples for short tandem repeats (STRs) failed. The sequencing of the mitochondrial hypervariable region I (HVR I) also failed. Nevertheless, it was possible to analyse the samples with mitochondrial DNA (mtDNA) single nucleotide polymorphisms (SNPs) via SNaPshot technique. The age of the analysed samples ranged from 2 months to 1400 years. Saliva-, blood-, sperm-, hair-, tooth- and bone-samples were investigated. Furthermore the mtDNA SNP analysis of a forensic case sample showing a mixed stain profile is presented. It was possible to discriminate two different haplogroups in this mixed-person stain. If compared to another mtDNA SNP profile that was found in a hair, the discriminating SNPs of the hair were as well found in the mixed-person stain.To disburden the SNP analysis in forensic casework, haplogroup assignment criteria and quality criteria for mtDNA SNaPshot analysis are announced.     

DNase-Ⅰ纯化结合碱性裂解法提取混合斑精子DNA

目的研究脱氧核糖核酸酶I(DNase-I)纯化结合碱性裂解法提取混合斑精子DNA的方法在法医学中的应用。方法收集79份性犯罪案件混合斑检材,分别用DNase-I纯化结合碱性裂解法和差异裂解法提取精子DNA,采用STR荧光标记复合扩增体系进行16个STR基因座分型,并比较检验结果。结果应用DNase-I纯化结合碱性裂解法提取精子DNA,64例检材分型成功;应用差异裂解法提取精子DNA,57例检材分型成功;两种方法比较结果存在显著性差异(P=0.039),DNase-1纯化结合碱性裂解法提取精子DNA的STR分型成功率更高,成本低廉。结论DNase-I纯化结合碱性裂解法提取混合斑精子DNA可提高检验成功率,操作简便,快速,易于自动化,适于法医学个体识别鉴定。     

基于二代测序的混合检材精细化分型研究

目的基于二代测序平台进行混合检材精细化STR分型,并评估其法医学应用价值。方法收集性侵案件中3例混合检材及其比对样本,采用M48磁珠提取纯化试剂盒提取样本DNA,使用Foren SeqTM DNA Signature Prep试剂盒制备文库,Mi Seq FGx平台进行测序,Foren SeqTM Universal Analysis v1.2.1软件进行数据分析,将STR序列多态分型与长度多态分型进行比较。结果对3例混合检材STR分型进行拆分,在D3S1358、D13S317与D9S1122基因座发现存在同一长度多态等位基因包含两个个体的序列多态等位基因的情况。结论二代测序技术可对混合检材进行精细鉴别,为混合分型数据拆分提供更多线索和依据。     

Citizen's psychological knowledge,legal knowledge,and attitudes toward participation in the new Japanese legal system,saiban-in seido

A new procedure, saiban-in seido, was introduced in the Japanese criminal court in 2009.1 1. This article was written before the Saiban-in seido started. A mixed tribunal of three professional judges and six lay people selected from a list of voters deliberate the verdict in serious criminal cases such as murder, rape, and arson. This study researched lay people's attitudes toward the new system, their psychological knowledge (e.g. the reliability of eyewitness testimony) and legal knowledge (e.g. ‘presumed innocent’), and the relationship between attitude and knowledge. Study 1 examined the responses of 294 citizens to a questionnaire; 90 responses were examined in Study 2 (both samples consisted of two age groups, i.e. (1) 20s and (2) 40s and 50s, and two education levels, i.e. (1) college or below and (2) university or more. In both studies, respondents showed concerns about their lack of ability and knowledge to become a lay judge. Although legal knowledge was related to attitude – i.e. the more legal knowledge, the less negativity – no relationship was found between psychological knowledge and attitude. Relevant support for citizens to become lay judges was discussed.     

The third wave: mixed migration from Zimbabwe to South Africa

Migration from Zimbabwe has recently been described as an archetypal form of “mixed migration” in which refugees and migrants are indistinguishable from one another. This paper argues that such a state-centred understanding of mixed migration oversimplifies a far more complex reality and fails to adequately account for the changing nature of Zimbabwean out-migration. Based on data from three separate Southern African Migration Programme (SAMP) surveys undertaken in 1997, 2005 and 2010 at key moments of transition, the paper shows how the form and character of mixed migration from the country has changed over time. The country’s emigration experience since 1990 is divided into three periods or “waves”. The third wave (roughly from 2005 onwards) has seen a major shift away from circular, temporary migration of individual working-age adults towards greater permanence and more family and child migration to South Africa. Zimbabwean migrants no longer see South Africa as a place of temporary economic opportunity for survival but rather as a place to stay and build a future for themselves and their families.     

Electoral coordination in mixed‐member systems: Does the level of democratic experience matter?

A number of institutional and non‐institutional factors hamper electoral coordination and, hence, increase party system fragmentation in the nominal tier of mixed electoral systems. Contrary to expectations, the number of electoral parties is not lower in all old democracies. Nevertheless, the level of democratic experience modifies the effect of other variables like the type of mixed electoral system or the closeness of the races. Econometric tests evaluate this phenomenon in a diverse sample of 15 countries and a total of 57 elections with more than 10,000 observations at the district level.