Cytoskeleton immunohistochemical study of early ischemic myocardium

Previous studies on cytoskeletal changes of in vitro and in vivo animal models of ischemic myocardium have suggested the possibility of using alterations in cytoskeleton proteins as an early marker for the post-mortem diagnosis of myocardial ischemia in cases of sudden death due to coronary artery disease (CAD). In the present study, using the technique of ABC-immunohistochemistry, we examine the changes of three cytoskeletal proteins: vinculin, desmin and α-actinin in human myocardial samples taken from 14 cases of CAD sudden death and 13 cases of non-CAD death. Results of these examinations are compared with immunohistochemical changes of myoglobin and histochemical staining of hematoxylin and eosin and phosphotungstic acid, and Masson trichrome. Patchy and extensive loss of the three cytoskeletal proteins was demonstrated in the myocardium of victims who died 1 h or later following the onset of symptoms of ischemic myocardium. The pattern of cytoskeleton change is equivocal in the cases of CAD who died less than 1 h after the onset of symptoms and of the cases of non-CAD. In these cases, no significant histological change was observed. With less non-specific background changes and stronger positive staining, immunohistochemical staining of the three cytoskeletal proteins is more reliable than myoglobin, which has attracted the attention of many pathologists searching for anatomic evidence of ischemic myocardium in coronary artery disease.     

中介素对大鼠急性心肌缺血损伤的作用及机制

目的 探讨中介素(intermedin,IMD)对大鼠急性心肌缺血损伤的作用及机制,为研究心源性猝死机制提供思路.方法 72只健康雄性大鼠随机分为对照组、缺血组、IMD干预组.酶化学法检测心血中乳酸脱氢酶(1actate dehvdrogenase,LDH)、丙二醛(malondialdehyde,MDA)、超氧化物歧...     

灯盏细辛与复方丹参注射液治疗脑梗死疗效比较的Meta分析

目的 系统评价灯盏细辛对比复方丹参治疗脑梗死的疗效。方法 计算机检索中国期刊全文数据库、中国生物医学文献数据库、万方数据库和维普数据库,并辅以手工检索和其他检索,收集灯盏细辛治疗脑梗死的随机对照试验,检索时间截至2013年7月。由2名研究者按照纳入与排除标准独立筛选文献、提取资料,并采用Jadad量表对纳入研究进行质量评价后,用RevMan 5.0软件进行Meta分析。结果 共纳入23个随机对照试验。Meta分析结果显示,灯盏细辛改善神经缺损的效果优于复方丹参,灯盏细辛对脑梗死的有效率高于复方丹参,灯盏细辛的不良反应轻微。结论 灯盏细辛可有效治疗脑梗死,其效果优于复方丹参,不良反应轻微。     

亳菊总黄酮对大鼠急性心肌缺血的保护作用

目的 研究亳菊总黄酮对急性心肌缺血模型大鼠心脏的保护作用,为其进一步开发提供实验依据。方法 将SD大鼠随机分成假手术组,模型组,复方丹参滴丸组（140 mg/kg）,亳菊总黄酮小、中、大剂量组（分别为75、150、300 mg/kg）。采用冠状动脉结扎法复制大鼠急性心肌缺血模型。分别于手术前,手术后0、0.5、1.0、2.0 h测定各组大鼠心电图ST段变化;冠状动脉结扎后24 h测定血清天冬氨酸转移酶（aspartate aminotransferase, AST）、乳酸脱氢酶（lactate dehydrogenase, LDH）、肌酸激酶（creatine kinase, CK）含量;观察各组大鼠心肌梗死率及心肌组织形态学变化。结果 亳菊总黄酮可降低急性心肌缺血大鼠各时点心电图ST段抬高值,降低血清AST、LDH、CK含量,降低心肌梗死率,改善心肌组织形态学变化,呈现出一定的剂量效应。结论 亳菊总黄酮对急性心肌缺血模型大鼠心脏有一定的保护作用。     

活血通脉片对实验动物血液流变性和高脂血症影响

目的：观察中药复方制剂活血通脉片对实验性心肌缺血犬的血液流变性和对ＳＤ大鼠实验性高脂血症的影响。方法：结扎冠状动脉，造成犬急性心肌缺血（“血瘀证”）动物模型，经十二批肠灌入受试药物，从犬心大静脉留取血样，动态观察实验性心肌缺血犬的血液流变性指标；连续喂高脂饲料２周，复制ＳＤ大鼠高脂血症模型，灌胃给予受试药物，于第１５ｄ测定实验性ＳＤ大鼠血脂指标。结果：活血通脉片能降低实验性心肌缺血犬的全血高切、低切粘度和血浆粘度，加快械细胞电泳时间，与对照组比较有显著差异（Ｐ〈０．０１）；能降低实验性大鼠血清ＴＣ及ＬＤＬ－Ｃ水平（Ｐ〈０．０１）。结论：活血通脉片能改善急性心肌缺血犬的血液流变性和实验性高脂血症大鼠的血脂。     

心脑血康胶囊治疗脑梗死30例临床观察

目的：观察心脑血康胶囊对脑梗死的治疗作用。方法：３０例脑梗死患者服用心脑血康胶囊，每次４料，每日３次，１个月为１个疗程，根据病人治疗前后神经功能缺损积分判定疗效。结果：基本痊愈６例，显著进步１５例，进步４例，无效５例，显效率７０％。结论：心脑血康胶囊治疗脑梗死有较好疗效。