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921.
The Application of Voltammetric Analysis of Δ9‐THC for the Reduction of False Positive Results in the Analysis of Suspected Marijuana Plant Matter
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Marco A. Balbino Ph.D. Laura S. de Oliveira M.S. Izabel C. Eleotério Ph.D. Erica N. Oiye M.S. Maria F.M. Ribeiro M.S. Bruce R. McCord Ph.D. Antonio J. Ipolito M.S. Marcelo F. de Oliveira Ph.D. 《Journal of forensic sciences》2016,61(4):1067-1073
The development of methodologies using inexpensive, fast, and reliable instrumention is desirable in illicit drug analysis. The purpose of this study was based on cyclic voltammetry technique to differentiate the electrochemical behavior of ?9‐THC, the psychoactive substance in marijuana, and five different extract plants to yield false positive results after analysis protocol for cannabinoids using thin‐layer chromatography and Fast Blue B salt. After applying a deposition potential of ?0.5 V in a glassy carbon working electrode, the results indicated an anodic peak current at 0.0 V versus Ag/AgCl after addition of ?9‐THC solution in the electrochemical cell, and limits of detection and quantification were 1.0 ng mL?1 and 3.5 ng mL?1, respectively. Other interfering plants showed distinct amperometric responses. This methodology was useful to detect ?9‐THC even in the presence of the Fast Blue B salt, which avoided false positive results for all the studied extract plants. 相似文献
922.
Application of mRNA Expression Analysis to Human Blood Identification in Degenerated Samples that were False‐negative by Immunochromatography,,
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Shusaku Matsumura M.S. Aya Matsusue Ph.D. Brian Waters M.S. Masayuki Kashiwagi M.D. Ph.D. Kenji Hara Ph.D. Shin‐ichi Kubo M.D. Ph.D. 《Journal of forensic sciences》2016,61(4):903-912
Forensic laboratories are often faced with cases in which methamphetamine hydrochloride‐mixed blood is unable to be identified as human blood by immunochromatography against human hemoglobin A0. The application of mRNA expression analysis to samples that showed a false‐negative with immunochromatography was investigated as an alternative approach that did not depend on the antigen–antibody reaction. Real‐time PCR was used to examine the expression levels of blood markers such as glycophorin A, spectrin beta, and hemoglobin beta. Hemoglobin beta was the only marker that was specifically detected in blood, while glycophorin A was useful for determining human specificity. Hemoglobin beta showed good detection sensitivity and was detectable in 37‐year‐old blood stains. Hemoglobin beta was exclusively detectable in methamphetamine hydrochloride‐mixed blood stains. Detergents and disinfectants did not significantly influence mRNA markers. The proposed mRNA expression analysis was suitable for human blood identification as an alternative method to immunochromatography. 相似文献
923.
Comparison of Dimensional Accuracies Using Two Elastomeric Impression Materials in Casting Three‐dimensional Tool Marks
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Zhen Wang Ph.D. 《Journal of forensic sciences》2016,61(3):792-797
The purpose of this study was to evaluate two types of impression materials which were frequently used for casting three‐dimensional tool marks in China, namely (i) dental impression material and (ii) special elastomeric impression material for tool mark casting. The two different elastomeric impression materials were compared under equal conditions. The parameters measured were dimensional accuracies, the number of air bubbles, the ease of use, and the sharpness and quality of the individual characteristics present on casts. The results showed that dental impression material had the advantage of special elastomeric impression material in casting tool marks in crime scenes; hence, it combined ease of use, dimensional accuracy, sharpness and high quality. 相似文献
924.
Forensic Investigation of Formaldehyde in Illicit Products for Hair Treatment by DAD‐HPLC: A Case Study
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Erica N. Oiye M.S. Maria Fernanda M. Ribeiro M.S. Leonardo L. Okumura Ph.D. Adelir A. Saczk Ph.D. Pietro Ciancaglini Ph.D. Marcelo F. de Oliveira Ph.D. 《Journal of forensic sciences》2016,61(4):1122-1125
The illegal use of formalin (commercial formaldehyde) in cosmetic products harms the health of individuals exposed to this substance. Over the last years, the commercial availability of these products, especially those containing irregular dosage of formaldehyde, has increased in Brazil. This work analyzes some products for hair treatment available in the Brazilian market and verifies their safety. The adopted analytical methodology involved sample derivatization with 2,4‐dinitrophenylhydrazine, followed by high‐performance liquid chromatography with ultraviolet detection (UV–VIS) at λ = 365 nm. The limit of quantification is 2.5 × 10?3% w/w, and the recovery tests were around 93%. Some of the samples contained high and illegal formaldehyde levels ranging from 9% to 19% (w/w) and others presented suitable concentrations of the analyte. On the basis of the results, this work discusses the efficiency and practicality of this analytical method for forensic purposes. 相似文献
925.
Samah F. Ibrahim M.D. Marwa Issak M.D. Amany A. Bayoumy M.D. Dina S. Abd El‐Fatah M.D. 《Journal of forensic sciences》2016,61(4):1007-1010
Wound age estimation is one of the most important forensic aspects. Troponin I (TnI) and many cytokines, for example, tissue plasminogen activator (tPA), are involved in wound inflammation and healing. Skeletal (TnI) and cutaneous (tPA) mRNA was detected using real‐time PCR in 25 female albino rats. They were divided into 5 groups: control and 4 injured groups. Injured groups were sacrificed 1, 6, 24, and 30 h after inflicting contused wound. The expression levels of cutaneous (tPA) were decreased significantly at 1, 6, and 30 h after contusion (71.7%, 30.7 and 16.9%), while the expression levels of skeletal (TnI) were increased significantly at 1 and 6 h post‐traumatic, then they gradually decreased until reaching normal levels at 24 h and assumed significantly lower levels at 30 h postcontusion. These results suggested that the determination of cutaneous (tPA) and skeletal (TnI) mRNA levels was useful for wound age estimation. 相似文献
926.
Development of a Library Search‐Based Screening System for 3,4‐Methylenedioxymethamphetamine in Ecstasy Tablets Using a Portable Near‐Infrared Spectrometer
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Kenji Tsujikawa Ph.D. Tadashi Yamamuro Ph.D. Kenji Kuwayama Ph.D. Tatsuyuki Kanamori Ph.D. Yuko T. Iwata Ph.D. Kazuna Miyamoto M.S. Fumiyo Kasuya Ph.D. Hiroyuki Inoue Ph.D. 《Journal of forensic sciences》2016,61(5):1208-1214
This is the first report on development of a library search‐based screening system for 3,4‐methylenedioxymethamphetamine (MDMA) in ecstasy tablets using a portable near‐infrared (NIR) spectrometer. The spectrum library consisted of spectra originating from standard substances as well as mixtures of MDMA hydrochloride (MDMA‐HCl) and diluents. The raw NIR spectra were mathematically pretreated, and then, a library search was performed using correlation coefficient. To enhance the discrimination ability, the wavelength used for the library search was limited. Mixtures of MDMA‐HCl and diluents were used to decide criteria to judge MDMA‐positive or MDMA‐negative. Confiscated MDMA tablets and medicinal tablets were used for performance check of the criteria. Twenty‐two of 27 MDMA tablets were truly judged as MDMA‐positive. Five false‐negative results may be caused by compounds not included in the library. No false‐positive results were obtained for medicinal tablets. This system will be a useful tool for on‐site screening of MDMA tablets. 相似文献
927.
Characterization of Printing Inks Using DART‐Q‐TOF‐MS and Attenuated Total Reflectance (ATR) FTIR
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Rhett Williamson B.Sc. Anna Raeva Ph.D. Jose R. Almirall Ph.D. 《Journal of forensic sciences》2016,61(3):706-714
The rise in improved and widely accessible printing technology has resulted in an interest to develop rapid and minimally destructive chemical analytical techniques that can characterize printing inks for forensic document analysis. Chemical characterization of printing inks allows for both discrimination of inks originating from different sources and the association of inks originating from the same source. Direct analysis in real‐time mass spectrometry (DART‐MS) and attenuated total reflectance Fourier transform infrared spectroscopy (ATR‐FTIR) were used in tandem to analyze four different classes of printing inks: inkjets, toners, offset, and intaglio. A total of 319 samples or ~ 80 samples from each class were analyzed directly on a paper substrate using the two methods. DART‐MS was found to characterize the semi‐volatile polymeric vehicle components, while ATR‐FTIR provided chemical information associated with the bulk components of these inks. Complimentary data results in improved discrimination when both techniques are used in succession resulting in >96% discrimination for all toners, 95% for all inkjets, >92% for all offset, and >54% for all intaglio inks. 相似文献
928.
John D. Jackson 《The Modern law review》2016,79(6):987-1018
This article examines the responses of national courts to the ECtHR's decision in Salduz v Turkey that suspects be provided with access to a lawyer before they are first interrogated by the police. It argues that harmonious application of human rights standards in criminal proceedings should build upon common values underpinning the procedural traditions of member states. ECtHR success in gaining acceptance for the principle of access to a lawyer during police interrogation, anchoring it in the privilege against incrimination, is contrasted with resistance towards giving the defence any active role during criminal investigations. It is argued that this resistance can be overcome by an appeal to safeguards that have long dominated the trial process. As the investigation phase increasingly determines the outcome of criminal proceedings, standards of fairness traditionally reserved for the trial process should be applied also to this phase in order to provide suspects with an effective defence. 相似文献
929.
An Assessment of Juvenile Drug Courts’ Knowledge of Evidence‐Based Practices,Data Collection,and the Use of AA/NA
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Logan A. Yelderman 《Juvenile & family court journal》2016,67(1):33-48
The use of evidence‐based practices (EBPs) has become a core component of juvenile drug courts (JDCs). This research, using a sample of JDCs listed with the National Association of Drug Court Professionals, tests two current assumptions in the field: 1) many JDCs do not use or are unaware of their use of EBPs and 2) JDCs tend to overuse sober support groups (e.g., AA/NA), which are thought to be inappropriate for youth. Results suggest that nearly all JDCs, in the sample, reported using EBPs; however, only about a quarter of them collected treatment data and knew the outcomes of the data. Also, only about half of the JDCs use sober support groups (predominantly AA/NA), and nearly all of the sober support groups were tailored toward youth. Overall, these findings suggest that the current assumptions in the field do not accurately reflect the practices reported by these JDCs. Implications are discussed. 相似文献
930.
Molecular Pathology of Pulmonary Edema in Forensic Autopsy Cases with Special Regard to Fatal Methamphetamine Intoxication
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Rui Zhao Ph.D. M.D. Dong Zhao Ph.D. M.D. Ye Xue M.D. Bao‐Li Zhu Ph.D. M.D. Da‐Wei Guan Ph.D. M.D. Xiao‐Li Xie Ph.D. M.D. Qi Wang Ph.D. M.D. 《Journal of forensic sciences》2016,61(6):1531-1537
Pulmonary edema is a common finding in fatal methamphetamine intoxication. However, the underlying mechanism remains poorly understood. This study investigated the molecular pathology of alveolar damage involving pulmonary edema in forensic autopsy cases. Seven candidate reference genes (RPL13A, YWHAZ, GUSB, SDHA, GAPDH, B2M, and ACTB) were evaluated in the lung by the geNorm module in qBaseplus software. RPL13A, YWHAZ, and GUSB were identified as the most stable reference genes. Using these validated reference genes, intrapulmonary mRNA expressions of matrix metalloproteinases (MMPs), intercellular adhesion molecule‐1 (ICAM‐1), claudin‐5 (CLDN‐5), and aquaporins (AQPs) were examined. Relative mRNA quantification using TaqMan real‐time PCR assay demonstrated higher expressions of all markers except for AQP‐5 in fatal METH intoxication cases. These findings suggested alveolar damage and compensatory response in fatal METH intoxication cases. Systematic analysis of gene expressions using real‐time qPCR is a useful tool in forensic death investigation. 相似文献