BAT在非离子型造影剂过敏反应中的诊断价值

目的通过测定免疫正常人、β-内酰胺类药物过敏者以及非离子型造影剂过敏者血液中CD63、肥大细胞羧肽酶A3(MC-CPA3)、人末端补体复合物SC5b-9,探讨嗜碱性粒细胞活化试验(basophil activation test,BAT)在非离子型造影剂过敏反应中的诊断价值。方法采用流式细胞术测定全血中嗜碱性粒细胞CD63表达率。采用ELISA法测定血清中MC-CPA3、血浆中SC5b-9含量。结果非离子型造影剂和β-内酰胺类药物过敏者血液中嗜碱性粒细胞CD63表达率、MC-CPA3以及SC5b-9含量均较免疫正常人升高(P0.05)。结论非离子型造影剂过敏者血液中嗜碱性粒细胞和肥大细胞发生活化,补体系统的激活也参与了非离子型造影剂过敏反应的发生。流式细胞术分析BAT可作为非离子型造影剂过敏反应的诊断方法。     

Public Value Dimensions: Developing and Testing a Multi-Dimensional Classification

Further integration of the public value literature with other strands of literature within Public Administration necessitates a more specific classification of public values. This article applies a typology linked to organizational design principles, because this is useful for empirical public administration studies. Based on an existing typology of modes of governance, we develop a classification and test it empirically, using survey data from a study of the values of 501 public managers. We distinguish among seven value dimensions (the public at large, rule abidance, balancing interests, budget keeping, efficient supply, professionalism, and user focus), and we find systematic differences between organizations at different levels and with different tasks, indicating that the classification is fruitful. Our goal is to enable more precise analyses of value conflicts and improve the integration between the public value literature and other parts of the Public Administration discipline.     

5种免提取试剂盒检验滤纸血痕结果的比较

目的探讨5种免提取试剂盒对滤纸血痕样本检验的效果。方法陈旧滤纸血痕(存放时间12～14个月)及新鲜滤纸血痕(存放时间小于1个月)各920份,分别随机分为5组。应用AGCU 17+1、Goldeneye 20A、Powerplex16HS、Identifiler Plus、Identifiler Direct 5种免提取试剂盒进行检验,对比各组检验结果。结果陈旧滤纸血痕5种试剂盒的检验成功率为98.91%～100%,各组间无差异(P>0.05);新鲜滤纸血痕,Identifiler Plus和Identifiler Direct试剂盒检验成功率高于AGCU17+1、Goldeneye 20A及Powerplex 16HS试剂盒(P<0.01);将样本做陈旧化处理后再用成功率较低的3种试剂盒进行检验,成功率分别升至100%、99.46%、99.46%;Identifiler Plus试剂盒扩增循环27次效果优于28次。结论本文5种试剂盒均可用于滤纸血痕的直接扩增检验,但使用AGCU17+1、Goldeneye 20A及Powerplex 16HS试剂盒需将新鲜血痕做陈旧化处理;Identifiler Plus试剂盒需将循环次数降为27次。     

中国改革深水区的法律试验新难题和基本思路——— 以中国(上海)自由贸易试验区的制度体系构建为主线

上海自贸区的法制环境建设是改革试验不可或缺的核心组成部分。但是,已步入改革深水区的此次制度创新的力度和尺度之大、难度之高,使得相应的这场法律试验面临极大的挑战。它在立法框架上的基本立场,是转向和接轨于国际标准规则的新多边主义;在执法体制上,期望通过简政放权而取得新政治体制的突破;在救济体系上,虽然相应司法机构的安排只能是“框架内的微调”,但其拥有更多的法之外权衡空间,以及一系列多元性的处置原则和通道。在此基础上,真正实现从“法律试验”到“制度转型”的进化,且不能忽视制度构建的微观视角,要充分考量改革后执法监管体制的可复制,以及法外之地的专业多元解纷机制。     

法医毒物检验技术方法的质量要求

本文通过对73个法医毒物检验方法进行综合分析,从技术方法的质量特性上对方法的建立与确认提出质量要求.即从内部质量和外部质量的角度,针对法庭科学领域毒物检验技术方法,阐述其功能性、可靠性、易用性和有效性四个维度要求.     

Psychopathy,intelligence and emotional responding in a non-forensic sample: an experimental investigation

This study examined the relationships between psychopathy (primary and secondary), intelligence and emotional responding in a sample of 50 university students, using a task measuring autonomic responses to 40 pictorial stimuli (20 neutral and 20 emotionally provoking). Results indicated no significant direct relationship between primary or secondary psychopathy and emotional response, or primary or secondary psychopathy and intelligence. However, a significant moderating effect of intelligence on the association between both psychopathy factors and emotional response was observed, indicating those scoring higher on psychopathy but with lower intelligence portray the expected emotional responses to the affective stimuli (primary: β?=??.56, p?β?=?.80, p?<?.001). These findings indicate abnormal reactivity to emotional stimuli in lower intelligence, higher psychopathic individuals, and suggest differing roles for the two facets of psychopathy in affective responsiveness deviations.     

A Simple Solubility Tests for the Discrimination of Acrylic and Modacrylic Fibers

In a crime scene investigation, single fibers play an important role as significant trace physical evidence. Acrylic fibers are frequently encountered in forensic analysis. Currently, acrylic and modacrylic are not discriminated clearly in Japan. Only results of FT‐IR, some of acrylics were difficult to separate clearly to acrylic and modacrylic fibers. Solubility test is primitive but convenient useful method, and Japan Industrial Standards (JIS) recommends FT‐IR and solubility test to distinguish acrylic and modacrylic fibers. But recommended JIS dissolving test using 100% N,N‐dimethylformamide (DMF) as a solvent, some acrylics could not be discriminated. In this report, we used DMF and ethanol (90:10, v/v) solvent. The JIS method could not discriminate 6 acrylics in 60 acrylics; hence, DMF and ethanol (90:10, v/v) solvent discriminated 59 of the 60 fibers (43 acrylic and 16 modacrylic fibers) clearly, but only one modacrylic fiber incorrectly identified as acrylic.     

Microcrystal analysis of cocaine hydrochloride and added adulterants

The objective of this project was to investigate the trends of changes in the crystal morphology of cocaine in the presence of the common adulterants, caffeine and lidocaine HCl. By performing gold chloride microcrystal tests on samples of cocaine with adulterants at 10, 20, and 50% concentrations, trends in the changes of the crystal morphology can be linked to specific adulterants and concentrations. For cocaine/caffeine mixtures, the trend is elongation of one axis, additional branching, and brown discoloration of the crystals. At 50:50 cocaine/caffeine mixtures, branched spherical crystals and long needles appear. The trends for cocaine/lidocaine mixtures include elongation of one axis with an X-shaped middle axis. The axes continue to grow and branching decreases until at 50%, spherical clusters of needles appear. These results indicate microcrystal analysis can be used as a novel method for presumptively identifying not only cocaine but also the identity and concentration of the adulterant.     

正交设计优选黄芩栀子合煎工艺

目的 正交设计优选黄芩栀子合煎工艺。方法 采用正交试验法,考察加水量、煎煮时间、煎煮次数对黄芩、栀子合煎液中栀子苷含量、黄芩苷含量及干膏得率的影响。结果 优选出的最佳提取工艺为：加8倍量水,提取3次,每次0.5 h。结论 所优选的工艺稳定、合理、可行,可为合理开发含有黄芩、栀子药对的复方制剂提供理论依据。     

茶多酚急性、慢性毒性实验研究

目的：观察对茶多酚的急性和慢性的毒性反应。方法：小鼠分５组,剂量比值为１：０．８,连续灌胃７ｄ。按Ｂｌｉｓｓ法计算茶多酚ＬＤ５０。大鼠分对照组,茶多酚高、中、低剂量组,连续灌胃９０ｄ。结果：茶多酚ＬＤ５０为２．４９９ｇ／ｋｇ。动物长期服用茶多酚未出现体重、血常规、肝肾功能及脏器组织的毒性变化。结论：茶多酚属低毒性,对大鼠０．８ｇ／ｋｇ·ｄ＾－１的剂量相当于成人用量的１００倍以上,提示临床用药量是安