探讨EOS染色剂对血迹DNA检验的影响

目的探讨被EOS染色剂处理后的血迹进行DNA检验的初步方法。方法制备EOS染色剂处理的血迹样本，分别采取纯水擦拭、75％酒精擦拭，或手术刀刮取血痕浸泡于纯水中、759／5酒精中，然后提取DNA进行下一步检测。结果采用刀刮取血痕浸泡于759／6酒精中，提取的DNA检测结果较好。结论初步实验显示，经EOS染色剂处理过的血迹，可参考刀刮取血迹置于75％酒精浸泡后提取DNA做下一步检测。     

牛新孢子虫病和弓形虫病的流行病学调查

为了调查我国牛新孢子虫和弓形虫的感染情况,应用新孢子虫酶联免疫吸附试验(ELISA)和弓形虫间接血凝试验(IAT)分别检测了来自国内10个省(市、自治区)的262份乳牛、10份肉牛和40份水牛血清。结果显示,乳牛新孢子虫的抗体阳性率为17.2%,弓形虫的抗体阳性率为2.3%,没有检测到既有新孢子虫抗体又有弓形虫抗体的乳牛血清。各牛场所检乳牛血清的新孢子虫抗体阳性率在0~34.4%之间。在水牛和肉牛血清中未检测到新孢子虫抗体和弓形虫抗体。流产乳牛血清的新孢子虫抗体阳性率为20.2%,未流产乳牛为16.1%,其中血清抗体阳性乳牛主要在妊娠中晚期流产。各个年龄段乳牛血清的抗体阳性率差异不显著(P>0.05)。不同妊娠胎次的乳牛血清抗体阳性率差异不显著(P>0.05)。确认新孢子虫病在国内大部分牛场存在。     

直接同步扩增5种基因位点

来用直接同步ＰＣＲ扩增５种基因位点及反向打点杂交技术，使扩增产物ＤＮＡ与膜探针条杂交。再通过酶联底物显色反应对ＬＤＬＲ、ＧＹＰＡ、Ｄ７Ｓ８、Ｇｃ和ＨＢＧＧ进行联合分型。该方法特别适用于微量、陈旧或降解的ＤＮＡ样本，其操作简便、迅速，应用在亲子鉴定案例，亲子关系概率高达９９．７５％。该技术在法医学、遗传学及临床诊断中具有重要价值，并将改变ＤＮＡ分型技术难于标准化的局面．     

5种免提取试剂盒检验滤纸血痕结果的比较

目的探讨5种免提取试剂盒对滤纸血痕样本检验的效果。方法陈旧滤纸血痕(存放时间12～14个月)及新鲜滤纸血痕(存放时间小于1个月)各920份,分别随机分为5组。应用AGCU 17+1、Goldeneye 20A、Powerplex16HS、Identifiler Plus、Identifiler Direct 5种免提取试剂盒进行检验,对比各组检验结果。结果陈旧滤纸血痕5种试剂盒的检验成功率为98.91%～100%,各组间无差异(P>0.05);新鲜滤纸血痕,Identifiler Plus和Identifiler Direct试剂盒检验成功率高于AGCU17+1、Goldeneye 20A及Powerplex 16HS试剂盒(P<0.01);将样本做陈旧化处理后再用成功率较低的3种试剂盒进行检验,成功率分别升至100%、99.46%、99.46%;Identifiler Plus试剂盒扩增循环27次效果优于28次。结论本文5种试剂盒均可用于滤纸血痕的直接扩增检验,但使用AGCU17+1、Goldeneye 20A及Powerplex 16HS试剂盒需将新鲜血痕做陈旧化处理;Identifiler Plus试剂盒需将循环次数降为27次。     

脑外伤认知障碍者神经心理学评价研究

目的探讨神经心理学检测指标在脑外伤者认知功能障碍评定中的应用价值。方法对脑外伤后认知障碍者进行中国修订韦氏成人智力量表、韦氏记忆量表及计算机化神经行为评价系统部分项目等神经心理学检测,按照原发性损伤程度和专家鉴定结论分别编组,对检测结果进行分析。结果损伤程度组填图、长时记忆、瞬时记忆、视觉保留及视简单反应时项目得分,轻型组高于中、重型组(P<0.05),其余项目(木块图、知识、相似、曲线吻合及数字筛选)组间差异无统计学意义。专家鉴定组中长时记忆、瞬时记忆、视觉保留、视简单反应时、曲线吻合及数字筛选得分值随认知功能障碍程度加重而逐渐降低,其中前4项组间差异具有统计学意义(P<0.05)。而韦氏智力测验4个项目较轻组与较重组得分接近,且均低于中度组,其中3项得分比较具有统计学差异(P<0.05)。结论原发性脑外伤与认知障碍严重程度有一定相关性,但相关检测项目显示不完全平行,在进行鉴定中应选择更为可靠的检测项目。     

Public Value Dimensions: Developing and Testing a Multi-Dimensional Classification

Further integration of the public value literature with other strands of literature within Public Administration necessitates a more specific classification of public values. This article applies a typology linked to organizational design principles, because this is useful for empirical public administration studies. Based on an existing typology of modes of governance, we develop a classification and test it empirically, using survey data from a study of the values of 501 public managers. We distinguish among seven value dimensions (the public at large, rule abidance, balancing interests, budget keeping, efficient supply, professionalism, and user focus), and we find systematic differences between organizations at different levels and with different tasks, indicating that the classification is fruitful. Our goal is to enable more precise analyses of value conflicts and improve the integration between the public value literature and other parts of the Public Administration discipline.     

HLA区域SNPs及其在法医学中的应用价值

人类白细胞抗原是迄今为止人类发现的最复杂的基因系统。HLA区域SNPs的分布频率高于人类整个基因组水平达到8.6%。目前SNPs可用多种方法检测。本文主要综述HLA区域SNPs在法医学亲权鉴定和个人识别的应用价值。     

Eu merger control and the compatibility test:a review of recent developments

The compatibility test contained in Article 2 of the Merger Control Regulation (MCR) is at the very heart of EU merger control, for it determines whether a concentration with a community dimension is deemed compatible or incompatible with the common market. Incompatibility can lead to prohibition of a concentration, although this is rare. The paper reviews the recent developments to the conditions of the test itself as well as the analytical methods employed to determine compatibility. Concerning the former, the new foreseeable dominance interpretation, put forward by the European Commission and made law by the Court of First Instance (CFI), is explored. This new variant of the dominance condition is important on its own right but it is also of major interest because of the explicit legal requirement placed on the Commission to assess the future likelihood of abusive behaviour by the merging parties in its prospective analysis. This is not the case with the original dominance compatibility condition. The unexpected but important clarification by the CFI of the notion of substantial part of the common market, as contained in the express wording of the compatibility test, is also commented upon. Concerning the determination of compatibility, the Commission's controversial employment in certain conglomerate concentrations of the range effects of competitive harm theory is examined, as is the need to take cognisance of merger specific efficiencies when determining if a merger increases societal welfare. The EU is making progress toward such an efficiencies assessment as part of the compatibility determination. EU merger control – and hence the compatibility test – do not exist in a vacuum. The EU has played a major role in shaping the new multilateral architecture and its goal of increasing international convergence in competition matters. This in turn has led the EU to rethink the nature of the compatibility test. For example, it has sought to evaluate the dominance condition of the compatibility test with the substantial lessening of competition (SLC) approach used by some other regulators, like the US. The paper concludes by looking at a fundamental issue that has arisen from recent CFI judgements and the GE/Honeywell merger: the competence of the Commission, or more accurately the Merger Task Force (MTF), to carry out the compatibility determination. Proposals are outlined so as to ensure that the Commission's prospective analysis in a concentration case meets the requisite legal standard. It is essential for this standard to be met if EU merger control is to remain credible. This revised version was published online in July 2006 with corrections to the Cover Date.     

VIEW FROM THE BENCH

Although some believe that the family courts are gender biased against fathers, Judge Menno disagrees. From his experience as a family court judge and his view from the bench, fathers are treated fairly in court determinations pertaining to access to their children. In making these determinations, courts must take into consideration the reality of divorce and out-of-wedlock arrangements. This article describes how Judge Menno's county family court operates, and he further subgroups various types of fathers, describing how each fares in the family court when trying to gain access to their children.     

踹门足迹的发现、提取与分析检验

踹门足迹是指犯罪嫌疑人用足撞击门 ,破坏锁体或门 ,使门锁分离或门板与门框分离而遗留在门面上的足迹。由于形成踹门足迹的承痕落体、作用力的大小与方向和正常步行足迹相比比较特殊 ,因此 ,它们在特征利用、分析方法和检验重点上有较大区别。