Assessment of intra- and intercostal variation in rib histomorphometry: its impact on evidentiary examination

Rib histological age estimation requires the evaluation of the middle third of the sixth rib. Human ribs have thin cortices and, when recovered, are often fragmented or absent, making it difficult to identify a specific midthoracic rib. This research explores the amount of microstructure variation in the middle third of the midthoracic ribs and determines whether the sixth rib age prediction equation can be applied to non-sixth ribs with similar accuracy. The amount of variability must be evaluated in order to meet the criterion for evidentiary examination. The sample consists of 120 cortical bone cross-sections from the middle third of ribs 3-8 removed from 20 cadavers. For each rib, osteon population densities (OPDs) and associated age estimates were calculated. The results demonstrate that non-sixth ribs can provide similar OPD values compared with those of the sixth ribs; however, individual variation proved to be significantly associated with bias, suggesting that individual factors influence the magnitude and direction of bias in non-sixth rib OPD values. This demonstrates the importance of evaluating multiple cross-sections (both intra- and inter-rib) to estimate age due to the normal remodeling variation within individuals.     

Identification of human semenogelin in membrane strip test as an alternative method for the detection of semen

Semenogelin (Sg), a protein originating in the seminal vesicles and a substrate for prostate specific antigen (PSA or p30), is a useful marker for the identification of semen. And detection of Sg has been available commercially in a membrane test recently. PSA is commonly used to detect semen in forensic significant samples taken from sexual assault cases. The strip PSA test has been available commercially from various manufacturers for many years. In this study, we evaluated two immunochromatographic membrane tests, one for Sg and the other for PSA by analyzing human semen, other human bodily fluids/materials including urine, blood, saliva, sweat, breast milk, vaginal secretion and fecal materials, semen from various animals and forensic casework samples. The data demonstrate that both Sg and PSA strip tests provide rapid and sensitive method for identification of seminal plasma. These results show that the immunochromatographic method for Sg detection is useful for the identification of seminal plasma in forensic samples, an alternative to the method for PSA detection.     

MiniSTR multiplex systems based on non-CODIS loci for analysis of degraded DNA samples

We describe two short amplicon autosomal short tandem repeat (miniSTR) quadruplex systems for eight loci D1S1171, D2S1242, D3S1545, D4S2366, D12S391, D16S3253, D20S161, and D21S1437, unlinked from the combined DNA index system (non-CODIS) loci, using newly designed primer sets. The results of an assay of 411 Japanese individuals showed that polymerase chain reaction (PCR) products within the eight loci were less than 150bp in size, without the seven additional bases for adenylation. The frequency distributions in the loci showed no deviations from Hardy-Weinberg equilibrium expectations. The accumulated power of discrimination and power of exclusion for the eight loci were 0.9999999991 and 0.998, respectively. For assay of highly degraded DNA, including artificially degraded samples and the degraded forensic casework samples assessed with the present miniSTR quadruplex systems, the systems proved quite effective in analyzing degraded DNA.     

户籍及其超越:北京流动人口服务与管理法治化之门

流动人口服务与管理,是我国"十二五"时期深化体制改革的具有战略意义的一个重要方面,也是北京当前社会管理创新必须面对并在实践中取得突破的一个关键领域。对我国流动人口尤其是北京流动人口问题予以梳理,提出大都市流动人口服务与管理之难,首先在于实有人口底数不清。为了落实和改进各项人口服务与管理的制度和措施,推动流动人口服务与管理进一步迈向法治化之路,在北京流动人口服务与管理的某些方面或某些领域,必须超越户籍问题。     

Sex estimation from the mastoid process among North Indians

Determination of sex from fragmentary crania is a critical problem in forensic anthropology. Osteometric analysis of mastoid can serve forensic anthropologists better in sex identification by virtue of the noticeable dimorphic characteristics. The present study aims to develop population-specific, sex-differentiating anthropometric standards for the mastoid process of North Indian skulls. Eight parameters of the mastoid region were measured on 138 adult crania (M/F 104:34, 22-65 years) and analyzed using SPSS 16.0. All parameters showed significant sexual dimorphism (p < 0.000). In stepwise analysis, asterion-mastoidale and mastoid breadth have provided an accuracy of 87%. Receiver operating characteristic curves were obtained for each variable to observe their overall performance in sex determination. Posterior end of incisura mastoidea-depression of suprameatal triangle was found to be the best variable with maximum area under curve and highest predictive accuracy (82.6%).     

Multiple stable isotope characterization as a forensic tool to distinguish acid scavenger samples

Acid scavengers are frequently used as stabilizer compounds in a variety of applications. When used to stabilize volatile compounds such as nerve agents, the lower volatility and higher stability of acid scavengers make them more persistent in a post-event forensic setting. Compound-specific isotope analysis of carbon, nitrogen, and hydrogen in three acid-scavenging compounds (N,N-diethylaniline, tributylamine, and triethylamine) were used as a tool for distinguishing between different samples. Combined analysis of multiple isotopes improved sample resolution, for instance differentiation between triethylamine samples improved from 80% based on carbon alone to 96% when combining with additional isotope data. The compound-specific methods developed here can be applied to instances where these compounds are not pure, such as when mixed with an agent or when found as a residue. Effective sample matching can be crucial for linking compounds at multiple event sites or linking a supply inventory to an event.     

The configuration of cervical spinous processes in black and white South African skeletal samples

The present study investigates the frequency of bifidity/nonbifidity in the black and white South African populations. Four-hundred skeletons of known race, sex, and tribal group were examined. Spinous processes were classified as bifid (bifurcate and cleft subtypes) and nonbifid (obtuse, pediculate, acinate, and clavate subtypes). Statistical analysis using the chi-squared probability test (p < 0.05) was carried out. Bifid spinous processes were present significantly more frequently in the white sample (58.9%) than in the black (31.6%). A bifid spinous process occurred most commonly in C2 (89%) followed by C5 (83%), C4 (79%), C3 (59.4%), and C6 (41.7%) in the white sample. A similar order of decrease in bifidity occurred in the black sample. C2 most commonly displayed the bifurcate subtype of spinous process, while the clavate subtype of nonbifid spinous process was the most common in both populations. The significantly higher frequency of bifidity in the white South African sample when compared to the black sample may suggest a population difference.     

Sex determination from the talus of Koreans by discriminant function analysis

The aims of this study were to investigate the sex discriminating potential of the talus in Koreans and compare this with other analyses in different populations. Statistical analyses were performed using data from nine measurements acquired from 140 tali (70 men, 70 women). The talus of Koreans is dimorphic between sexes in all measurements (p < 0.01). Discriminant function equations were generated by univariate, multivariate, and stepwise methods with a range of accuracy from 67.1 to 87.1%. Stepwise equations of other populations did not discriminate the sex of the Korean sample as accurately as each equation's own accuracies. The variables with high accuracy in this study are useful for sex determination of Koreans on the basis of confirmation of population specificity.