新闻网站可持续发展的盈利模式探讨

针对我国新闻网站普遍面临的有社会效益而无经济效益的生存困境,文章提出了我国新闻网站可持续发展的六大盈利模式,并分析了各自的利弊,提出了相应可持续发展的策略。     

县域金融发展与产业集聚区经济增长——以浙江为例

本文以浙江为例,在经济增长理论的基础上,建立了县域金融发展与产业集聚区经济增长之间相互作用的内生模型,基于浙江省22县（市）的县级数据,运用面板数据模型对县域金融发展与产业集聚区经济增长的互动关系进行了实证检验,比较了各产业集聚区经济增长的影响因素。研究发现：浙江县域金融发展规模、固定资产投资、实际利用外资与产业集聚区经济增长存在高度正相关关系;金融发展效率对产业集聚区经济增长的检验效果不显著;实际利率对产业集聚区经济增长的影响不显著,提出在执行利率政策时,不能采用金融发展理论中提高利率、解除金融抑制的政策手段。     

Effect of financial sector development and FDI inflow on employment rate in South Asia: New empirical evidence from ARDL approach

The current analysis aims to explore the empirical nexus between financial development, foreign direct investment (FDI) inflow, and employment rate. To attain this aim, we collect 30 years of annual data over the period 1990 to 2019 from South Asian economies and employ the autoregressive dynamic least square (ARDL) model for regression analysis. The implication of the ARDL model was subject to the mixed stationarity status of the series as assessed by unit root testing. The robustness of the analysis was checked by employing the fully modified ordinary least square (FMOLS) and dynamic ordinary least square (DOLS) models. The statistical analysis infers that both financial development and FDI inflow enhance the employment rate in the South Asia region. In addition, the empirical analysis infers that the gross capital formation, economic growth, and export volume have a positive while the population growth rate has a negative effect on the employment rate. The impact of underlying explanatory variables was found significant only in long run. The estimated coefficient values in the case of FMOLS and DOLS models support the direction of the relationship between explanatory variables and employment rate, implying the robustness of the analysis. The findings of the current analysis can be used to devise efficient economic policies to cope with the encroaching issue of unemployment in the South Asia region. This study offers the robustness to existing literature and complements the literature by exploring the underlying arrangement of study to the whole South Asia group.     

猪生殖与呼吸综合征病毒宁夏分离株ORF5基因的克隆表达及其表达产物的活性分析

参考已发表的猪生殖与呼吸综合征病毒(PRRSV)ORF5基因序列,设计合成了1对覆盖完整ORF5基因区段的引物,克隆了PRRSV NX/HY株的ORF5基因(登录号为EU600287),并进行了序列比较.将该基因克隆到原核表达栽体pGEX-6P-1中,构建了融合表达质粒pGEX-6P-1-5并转化BL21,对表达蛋白进行Western-blot分析.结果表明,NX/HY分离株ORF5基因与PRRSV JX1、CH-la株的核苷酸序列同源性分别为99.2%和95.2%,推导的氨基酸序列的同源性分别为98.5%和92.5%;层析扫描分析显示,目的蛋白的含量约占菌体总蛋白的30%,且具有良好的反应原性.GP5蛋白的表达为建立相应的血清学诊断方法奠定了基础.     

猪圆环病毒2型对体外培养仔猪淋巴细胞转化能力及其活性的影响

采集5头PCV2和PRRSV血清抗体阴性的断奶仔猪血液,分离外周血淋巴细胞,分对照组、PHA组、LPS组、PCV2组、PCV2+PHA组、PCV2+LPS组进行体外培养,用MTT法检测PCV2作用2d后对仔猪外周血淋巴细胞转化能力的影响;无菌取脾制成单细胞悬液,分单独淋巴细胞(L)、淋巴细胞+PCV2(L+V)、淋巴细胞与巨噬细胞(L+M)和淋巴细胞与巨噬细胞+PCV2(L+M+V)4组进行体外培养.分别在培养后第0、6、12和24 h收获淋巴细胞,用RT-PCR法检测淋巴细胞IL-2R(α)mRNA的表达.结果显示,与对照组、PHA组和LPS组相比,PCV2组、PCV2+PHA组和PCV2+LPS组淋巴细胞的转化能力均极显著降低(P＜0.01);PCV2对单独培养的淋巴细胞IL-2R(α)表达的影响不显著(P＞0.05),而巨噬细胞能协同PCV2促进淋巴细胞IL-2R(α)的表达,尤其在攻毒后第12和第24 h极显著增加(P＜0.01).证实PCV2在攻毒后第2 d能抑制体外培养T、B淋巴细胞的转化能力,且巨噬细胞能协同PCV2促进T淋巴细胞的活化.     

T-S连续模糊系统的一些新的稳定性问题研究

针对T-S连续模糊系统的稳定性和镇定问题,本文重点总结了T-S模糊系统的几个稳定性条件,从理论上比较他们所用方法的保守性和可实现性,并运用Matlab工具通过仿真实验,说明各个结论之间的有效性区别.     

Modelling the determinants of electronic tax filing services’ continuance usage intention

The success of electronic filing services largely depends on their continuance usage. This study examines the factors affecting the continuance usage intention of the online tax filing services in Mauritius. An integrated model comprising Trust Theory and Information System Success Model (ISSM) is applied to assess the continuance usage behaviour of e-filing systems. The model has been extended by adding two additional variables: Perceived Usefulness and Perceived Risk. The model was tested using a sample of 315 users of e-filing services in Mauritius. A structural equation modelling technique using partial least square structural equation modelling verified the hypotheses. The results reveal that the continuance usage intention of an electronic tax filing system is influenced by Perceived Usefulness, User Satisfaction, and Service Quality. However, Perceived Risk does not influence the continuance usage intention of e-filing systems since the importance of Perceived Risk diminishes as trust in the e-service provider increases. The theoretical and practical implications derived from the findings of this study are also discussed. This paper makes several contributions to the literature on electronic tax filing systems.     

Beyond the Z-score: A novel measure of bank stability for effective policymaking

This article looks beyond Z-score and proposes a novel methodological framework to build an all-encompassing indicator of bank stability for individual banks using the optimisation-based ‘benefit-of-the-doubt (BoD)’ approach. Unlike other available approaches, this approach is totally data-driven and generates endogenous weights to aggregate sub-indicators of bank stability and dimensions. Further, the final outcomes are not limited to a scalar measure of bank stability. The unique optimal weights offer valuable policy-relevant insights and highlight the most precarious areas of stability, which demand the immediate attention of the bank's management and the industry regulators for both micro-and macro-level policymaking. Using the data of Indian public sector banks operating in the year 2018, the study illustrates the proposed framework to obtain a holistic indicator of bank stability, defined on 14 ratio sub-indicators and 5 broad dimensions (soundness, asset quality, profitability, management efficiency, and liquidity) of bank stability.     

农村土地流转：特征、问题与对策——以陕西“高陵模式”为例

近年来,我国农村土地流转呈加速发展趋势,但还存在不少问题。陕西省高陵县从本地实际出发,通过探索土地流转的多种有效途径,形成了富有特色的＂高陵模式＂。当前,应借鉴＂高陵模式＂,推动农村土地流转的健康发展：充分尊重农民意愿,保护农民利益;政府要注重政策创新,规范管理土地流转活动;探索适合本地发展的模式;充分发挥中介组织的作用。     

猪轮状病毒SYBR GreenⅠ实时荧光定量PCR检测方法的建立

根据GenBank中登录的A群猪轮状病毒(PoRV)代表株OSU毒株序列,针对VP7基因设计了1对特异性引物,将150bp目的片段与pMD18-T载体连接构建标准品,建立了检测A群PoRV的SYBR GreenⅠreal-timePCR方法,并对该方法的敏感性、特异性和重复性进行了评价。结果显示,该方法在1.3×108copies/μL～1.3×102copies/μL范围内线性关系良好,相关系数为0.999;最低检测限为1.3×101copies/μL;用该方法检测猪瘟病毒、猪繁殖与呼吸综合征病毒、猪传染性胃肠炎病毒和猪病毒性腹泻病毒均为阴性;批内及批间变异系数均低于2%。表明,该方法敏感性高、特异性强、重复性好。用该方法抽检58份疑似病例的腹泻病料,结果检出53份阳性,同时用普通RT-PCR和快速检测试剂盒对这些病料进行检测,结果分别检出42份和32份阳性。用这3种方法对11例已知阳性的临床粪便样品进行检测,结果建立的检测方法与其他两种方法的符合率为100%。用该方法检测PoRV细胞培养物中的病毒载量为1.0×105copies/μL。结果表明,建立的检测方法为PoRV的早期诊断、分子流行病学调查以及疫苗质量的监测等提供了新的快速检测技术。