Positive identification of the principal component of a white powder as scopolamine by quantitative one-dimensional and two-dimensional NMR techniques

An unidentified white powder collected as evidence in an intelligence investigation was characterized exclusively by nuclear magnetic resonance (NMR) analysis. A small fraction of the powder dissolved in D2O was subjected to a series of one- and two-dimensional techniques which were used to elucidate the molecular structure of the powder's major component and positively identify it as the scopolamine biotoxin. Quantitative one-dimensional experiments identified individual proton and carbon atom sites, and conventional 14N spectroscopy detected a single nitrogen atom site. Heteronuclear single quantum coherence data correlated all protons to their directly bonded carbon atom, and together with the quantitative spectra, were used to determine the number of protons directly bonded to each carbon atom. The presence of a methyl, carboxyl, and a benzyl group was also identified from these data. Correlation spectroscopy detected a three proton and a nine proton JH,H network, representing a CH2CH moiety and seven carbon atom ring, respectively. These five elements were assembled into an almost complete molecular structure by using long-range, J-coupled, 1H-13C pairs detected by heteronuclear multiple bond correlation (HMBC) spectroscopy and 1H-1H dipolar-coupled pairs found from nuclear Overhauser effect spectroscopy (NOESY) data. Additional oxygen atom sites were inferred from 1H-13C correlation intensities in the HMBC spectra along with 1H and 13C chemical shift values, or directly from NOESY correlations. Only a single oxygen atom site could not be inferred from NMR data, but its presence was inferred from comparisons to target analyte structures to complete the structure of the scopolamine molecule. To confirm these results, an ethanol/H2O solution of the powder was analyzed by direct infusion into an ion trap mass spectrometer. A prominent base signal was observed at m/z 304.1 amu, corresponding to the protonated molecular ion of scopolamine. Subsequently, the ion was selected and subjected to collision-induced dissociation, producing characteristic major MS/MS fragments at m/z 138.1 and 156.1. Comparisons of 1H and 13C chemical shift values and JH,H values measured from our NMR data were found to agree very favorably with previously reported values for scopolamine in D2O.     

呼和浩特地区夏秋季常见嗜尸性蝇类的研究

目的研究呼和浩特地区常见嗜尸性苍蝇种类及其出现规律。方法观察法研究7～10月份兔尸上嗜尸性苍蝇种类变化。结果该地区兔尸上出现的双翅目嗜尸性苍蝇3科8属10种,主要有蝇科的家蝇、舍蝇、开普黑蝇、刺足齿股蝇、厩腐蝇;丽蝇科的丝光绿蝇、大头金蝇、铜绿蝇;麻蝇科的棕尾别麻蝇、肥须亚麻蝇。上述蝇类在尸体上出现时间及部位表现出很强的规律性。结论研究结果有望成为法医在呼和浩特地区对尸体进行死亡时间推断的依据。     

成都市嗜尸昆虫种类季节变化研究

目的 研究成都市嗜尸昆虫种类及其与季节变化的关系。方法 经过两年重复试验,观察3～11月兔尸上嗜尸昆虫种类变化。结果 发现3～11月兔尸上出现的嗜尸昆虫种类主要有舍蝇、丝光绿蝇、大头金蝇、赭尾麻蝇、巨阿尾丽蝇。其中,巨阿尾丽蝇见于3、4月,舍蝇出现于4月中旬至10月初,而大头金蝇、丝光绿蝇、赭尾麻蝇则自3月至10月中下旬均可见到,10月底以后则见不到任何嗜尸苍蝇。幼虫的出现晚于成虫1～4天不等,与季节及环境有关。结论 通过舍蝇、丝光绿蝇、大头金蝇、赭尾麻绳、巨阿尾丽蝇的生命周期的研究,有望为成都市法医学实践中死亡时间的推断提供依据。     

尸体玫瑰齿现象与死因的关系

目的研究尸体玫瑰齿现象与死因的关系及其法医学意义。方法采用观察法研究各种死因的大鼠尸体玫瑰齿现象。每种死因观察10只大鼠,其中8只死后即刻取牙齿观察(非腐败组),2只死后置水中腐败出现尸臭时取牙齿观察(腐败组),观察后立即将牙齿置于酒精中浸泡,分别于不同浸泡时间再进行观察。结果各种死因非腐败组即刻取牙观察均未见玫瑰齿现象,但置酒精中浸泡后均逐渐出现玫瑰齿现象,并在浸泡4h后玫瑰齿颜色显著;腐败组取牙齿后即刻观察均见玫瑰齿现象,置酒精浸泡1h后颜色不改变;腐败组较非腐败组颜色明显。结论尸体玫瑰齿现象与死因无关,不能作为法医学鉴定死因的依据。     

骨盆损伤鉴定的影像学检查方法选择探讨

目的通过研究骨盆结构和常用影像学检查方法,对骨盆损伤的影像学检查方法的选择提出建议。方法研究数例法医学鉴定中骨盆损伤的案例,结合骨盆环的生物力学和骨盆损伤分型,并对X线透视、X线摄片检查、CT等检查方法进行比较。结果四个骨盆损伤案例都是多发损伤,通过X线和CT检查均能满意地确认。结论结合骨盆致伤方式和生物力学,注意被鉴定人是否存在多发伤,来选择适合的影像学检查方法以正确评价骨盆损伤。     

眼眶骨折的影像学诊断及法医学评定

在眼眶骨折的法医学鉴定中常常出现漏诊和误诊。目前影像学技术在研究眼眶骨折的法医学特点和评价外伤的严重程度中有重要的意义。本文综述了眼眶骨折的分类、发病机制和影像学诊断的特点,对于眼眶骨折的法医学评价和鉴定有较好的帮助。     

利用数字图像处理技术伪造文件的研究

介绍了利用数字图象处理技术剪贴伪造文件、变造签名、彩色打印伪造原始印文和签名等三种伪造文件的方法及其特点.数字图象处理技术能够克服许多传统伪造方法的"缺点",使伪造的文件达到较高的逼真度.讨论了数字图象处理技术的滥用引发的新问题.     

判断溺水豚鼠在水中死亡时间的研究

乙醇和正丁醇大量存在于溺水尸体中。其浓度与尸体在水中的浸泡时间有一定的相关关系。温度能影响上述的相关关系。在自然平均温度１５—１８℃的条件下，运用气相色谱法测定其溺死后不同浸泡时间在血、肺、肝中存在的乙醇和正丁醇含量。其合并后回归方程式依次为ｙ１＝１１５．７２＋１８．２４ｘ；ｙ２＝－４１９．７１＋６４．７０ｘ；Ｙ３＝－３０３．０７＋５４．８７ｘ。研究中发现，溺死后７—１３天内，运用本方法来判断溺死时间的准确性较好。     

The theoretical aspects of the time dependent Z equation as a means of postmortem interval estimation using body temperature data only

It has been clearly demonstrated that the rectal cooling curve does not obey Newton's Law, which is exponential. The first success in modelling rectal cooling mathematically was achieved by Marshall and Hoare [1]. An amendment was made to the simple exponential curve which led to a good mathematical model, exhibiting the three main sections of rectal cooling, i.e. lag, linear and quasi-exponential. The resultant method of postmortem interval estimation required a knowledge of the body mass and height. The present study has led to a totally different amendment to Newton's Law, which provides a means of postmortem interval estimation from body temperature data only. The derivation of the method, with a background on Newton's Law follows.     

Postmortem diagnosis of syphilitic aortitis, including serological verification on postmortem blood

304 postmortem blood samples were serologically examined for syphilis at Statens Seruminstitut. Seventy-six percent of the samples were usable and gave clear-cut results (18% positive, 34% negative and 24% borderline). Twenty-four percent were indeterminable. The blood samples originated from 304 medico-legally examined bodies, 301 of whom were autopsied. The indications for the serological examinations were suspicion of syphilitic aortitis (SA) on the basis of the macroscopical appearance of the aorta at the autopsy--or information on earlier syphilis. Ninety-seven cases of SA were verified macro- and microscopically, 53 being lethal. The serological results were positive in half of these 97 cases, negative in 15, borderline in 21 and indeterminable in 12. A little more than half of the 97 cases were known in advance in the nation-wide syphilis index at the State Serum Institute. The variegated findings in the remaining part of the material are discussed. It is concluded that serological examination of postmortem blood may be a valuable adjuvant for the pathologist for the diagnosis of SA and other syphilitic manifestations.