Actin、Dm、Mb和Fn诊断急性心肌梗死的研究

目的探讨Actin、Dm、Fn和Mb在诊断急性心肌梗死中是否具有实际应用价值。方法选取已确诊的30例尸检心脏标本,分为急性心肌梗死组、冠状动脉粥样硬化组及正常心肌组。在心脏左前壁取材,连续切片,分别作HE染色,Actin、Dm、Fn和Mb免疫组化SP法染色,采用图像分析技术客观测量及比较各组标本染色强度。结果在急性心肌梗死组和冠状动脉粥样硬化组中,Actin、Dm和Mb在心肌细胞中表达均明显缺失,而Fn明显表达;而且在这两组间此4项指标表达效果无统计学差异。在正常心肌组中,Actin、Dm和Mb在心肌细胞中表达均无缺失,而Fn不表达。结论Actin、Dm、Fn和Mb对心肌细胞的缺血缺氧十分敏感,能反映明确的心肌坏死,但对坏死发生的机制缺乏特异性,因此用于诊断急性心肌梗死时需慎重。     

小鼠死后骨骼肌、心、肝、肾、脑细胞核中DNA的变化规律

目的 研究小鼠器官细胞核中DNA降解变化与死亡时间的关系,为法医学推断死亡时间提供一种新方法.方法 应用单细胞凝胶电泳技术结合荧光显微镜和计算机图像分析技术,测定小鼠在死后72h内不同时间点骨骼肌、心、肝、肾、脑细胞核头DNA含量、尾DNA含量、头半径、尾长度、尾矩、Olive矩、头面积、尾面积8项参数的变化值.结果 在小鼠死亡72h内,彗星尾DNA含量、尾长度、尾矩、Olive矩、尾面积都呈增加趋势,头DNA舍量、头半径、头面积均呈下降趋势.计算出体现DNA降解趋势的二项式回归方程(P<0.001)和多元回归方程(P<0.0001).结论 本研究提供的各组回归方程,可为法医学推断死后经过时间提供一种新的方法.     

大鼠心肌组织中microRNA和18S rRNA的降解与死亡时间的相关性

目的探讨大鼠死后心肌组织中microRNA和18S rRNA的含量变化与死亡时间的关系。方法将SD大鼠断颈处死后置于25℃室温、50%湿度的环境中,于死后不同时间提取心肌组织中总RNA。利用实时荧光定量PCR检测大鼠心肌组织中miR-1-2和18S rRNA的含量变化,结果用循环阈值(Ct值)表示,分析死亡时间与Ct值的关系,最终建立死亡时间推断的回归分析方程。结果大鼠死后120h内心肌组织中miR-1-2含量无明显变化,此后开始下降;而18S rRNA含量在96 h内逐渐增多,随后开始缓慢下降。大鼠死后不同时间18S rRNA的Ct值以及18S rRNA和miR-1-2的ΔCt值与PMI呈非线性相关,二次曲线拟合的R2值分别为0.9487、0.8072。结论 18S rRNA的Ct值和18S rRNA与miR-1-2的ΔCt值与PMI之间存在明显非线性关系,可以作为早期死亡时间推断的指标。     

Regulating Assisted Reproduction in Canada,Switzerland, and the USA: Comparing the Judicialization of Policy-Making

This article analyses the extent to which courts shape policies for assisted reproduction. While the USA is considered to be the most litigious country, Canada has observed a growing involvement of the courts from the 1980s onward, and Switzerland is characterized by a modest degree of judicialization. Based on national patterns, we would expect litigation and court impact to vary across these three countries. As the empirical analysis reveals, policy-process-specific variables such as the novelty of regulation, self-regulation by key stakeholders, and the policies in place better explain the variation in the judicialization of policy-making.     

Stature Estimation from Postmortem CT Femoral Maximum Length in Contemporary Danish Population

Stature estimation methods for Danish adult population have generally relied on Trotter and Gleser’s and Boldsen’s regression equations that are based on the skeletal remains of recent war dead American Whites, Terry Skeletal Collection, and Danish archaeological medieval skeletal materials, respectively. These equations are probably not suitable for stature estimation in contemporary Danish forensic cases. Furthermore, because postmortem computed tomography (PMCT) is now routinely performed at Danish forensic departments, equations based on PMCT, rather than measurements of defleshed bones, are needed. The aim of this study was to develop new equations for adult stature estimation based on PMCT femoral measurement. Maximum femoral length was measured on the PMCT images of 78 individuals (41 males and 37 females) aged 23–45 years. The measurement accuracy was tested on dry bones, and all the measurements were included in the inter- and intra-observer analyses. Both analyses results demonstrated the reliability of the method and data. Comparison between the living stature of the individuals and the estimates based on the equations by Trotter and Gleser and Boldsen demonstrated the unreliability of the previous equations to some extent. New regression equations were then developed and validated on a different sample of 18 Danish forensic cases. Comparisons of all the equations indicated that both the sets of previous equations underestimated the stature in the new validation dataset. The new equations developed in this study provide a reliable alternative for stature estimation in modern Danish forensic cases.     

Hemorrhagic Retinopathy and Optic Nerve Sheath Hemorrhage Associated With Fatal Subarachnoid Hemorrhage from a Ruptured Intracranial Aneurysm Due to Segmental Fibromuscular Dysplasia

Fibromuscular dysplasia is an idiopathic, nonatheromatous, and noninflammatory arterial disease that most commonly affects the renal and carotid arteries. We report a child with subarachnoid and ocular hemorrhage associated with an aneurysm due to fibromuscular dysplasia. Computed tomography following a witnessed collapse revealed diffuse subarachnoid hemorrhage and severe cerebral edema. An autopsy confirmed the radiographic findings and detected bilateral retinal hemorrhages, optic nerve sheath hemorrhages, and a ruptured saccular aneurysm due to focal fibromuscular dysplasia involving the intracranial right vertebral artery. This case documents a fatal subarachnoid hemorrhage in a child with an intracranial saccular aneurysm caused by fibromuscular dysplasia. The associated retinal hemorrhages are easily detected by postmortem monocular indirect ophthalmoscopy.     

Ma Rainey’s phonograph

This essay engages a series of performance routines by the blues artist Gertrude “Ma” Rainey, as a way of critiquing the epistemological tenets of sound reproduction technology. Between 1923 and 1925 Gertrude “Ma” Rainey carried out an elaborate quasi-burlesque performance routine in which she sang while hidden inside a giant phonograph; this routine precisely references and troubles the legacy of black sounds and bodies and their conflicted forms of capture andembodiment through sonic technologies. I think about how black sounds and bodies have been rendered as documentary objects within sonic and visual performance contexts and how this history is both referenced and complicated in Ma Rainey’s performances. I argue further that Rainey’s performance illustrates how sonic technologies always require, what I term black documentary embodiment, through the sonic and the visual. To this end I illustrate how reducing black art to an evidentiary object was central to the phonograph’s material and epistemological construction. In addition to analyzing Rainey’s performance I briefly engage the contemporaneous visual culture of the blues as a means to trace the legacy of black documentary embodiment within a longer visual-sonic tradition.     

Performance's mode of reproduction I: searching for Danh Võ’s mother

This essay returns to key debates in performance theory regarding the relationship between performance and reproduction, offering a Marxian inflected theory of performance’s mode of reproduction. It suggests that both performance theory and Marxist theory appropriate the mother function in describing performance’s mode of reproduction and the reproduction of capital, while displacing the mother in the process. Through a reading of the work of contemporary artist Danh Võ, the essay explores how performance’s mode of reproduction can affect the reproduction and sustenance of minoritarian life against historical, social, and economic forces of elision, erasure, and annihilation.     

从“家庭”走向“社群”——“都市中产全职妈妈”社会参与的路径与价值探析

两百年来的妇女解放道路一直强调妇女要走出家庭、参与生产,但却并未真正回应现代社会养育责任私人化所带来的双重负担问题。本文通过对四川省成都市一个以全职妈妈为主体的志愿者服务队的成立背景、发展路径、积极影响与限制因素的考察,剖析了现代社会城市女性面临的孕产和育儿困境,以及她们如何借助外部环境和资源优势走出私人家庭内部空间,在社区层面上进行支持与互助。这类群体的社会参与和自我成长也为我们反思"家庭—市场"二元悖论下的女性困境及其突破提供了新的可能与方向。     

Postmortem Fluid Concentrations of Heroin Biomarkers and Their Metabolites

Only limited data exist concerning the utility of complementary specimens in heroin-related deaths. As such, this report employed a validated LC-MS-MS method to quantify 6-monoacetylmorphine (6-MAM), 6-acetylcodeine (6-AC), and their metabolites morphine and codeine in blood with (BN) and without preservative (B) and the additional unpreserved specimens of vitreous humor, urine, stomach contents, and bile from 20 postmortem cases in which heroin was the primary cause of death. The median concentration of 6-MAM in BN was 0.011 mg/L, B was 0.008 mg/L, urine was 0.186 mg/L, vitreous humor was 0.022 mg/L, stomach contents was 0.147 mg/L, and bile was 0.012 mg/L. Only one case was found to be positive for 6-AC in B (case 6, 0.002 mg/L), and the median concentration of 6-AC was 0.002 mg/L in BN, 0.012 mg/L in urine, 0.003 mg/L in vitreous humor, 0.057 mg/L in stomach contents, and 0.004 mg/L in bile. These findings present new information on the distribution of these analytes in complementary matrices and support their inclusion for accurately determining the role of heroin in opioid-related deaths.