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871.
《Science & justice》2020,60(6):567-572
Forensic DNA profiling is a standard method used in the attempt to identify deceased individuals. In routine investigations, and if available, the preferred sample type is usually blood. However, this requires the invasive re-opening of the body, days or weeks after the autopsy, which is undesirable in resource-constrained mortuary settings. Motivated by the ease of sampling as well as reduced health and safety risks, this study aimed to establish the success rate of generating a full DNA profile on first attempt from buccal swab lysates using a direct PCR approach. Buccal swab samples were collected from 100 unidentified deceased males, and were subjected to direct DNA profiling with use of the Promega PowerPlex® Y23 Kit. At the time of sample collection, these individuals had been stored for between 1 and 887 days. This study shows that full DNA profiles were initially obtained from 73% of samples, which constitutes the first empirical data pertaining to first time success rates of direct PCR from post-mortem buccal lysates. Further investigation of partial and failed DNA profiles using real-time PCR showed that samples did not contain PCR inhibitors, DNA was not degraded, but DNA concentration was particularly low. Repeating DNA profiling with increased lysate input and extra PCR cycles yielded an additional six full DNA profiles, resulting in an overall success rate of 79%. Overall, DNA profile success rate was not associated with the duration of storage (p = 0.387). Lastly, massively parallel sequencing with the ForenSeq™ Signature DNA Prep kit provided more informative profiles for three additional samples. These results indicate that blood should therefore remain the sample of choice in a post-mortem setting, yet buccal lysates hold potential to be optimised further, which may ease the human identification workflow. 相似文献
872.
Jasmina Čakar Ph.D. Amela Pilav M.A. Mirela Džehverović B.A. Anesa Ahatović M.A. Sanin Haverić Ph.D. Jasmin Ramić M.Sc. Damir Marjanović Ph.D. 《Journal of forensic sciences》2018,63(1):295-298
The floods in Bosnia and Herzegovina in May 2014 caused landslides all over the country. In the small village of ?eri?i, near the town of Zenica, a landslide destroyed the local cemetery, relocated graves, and commingled skeletal remains. As the use of other physical methods of identification (facial recognition, fingerprint analysis, dental analysis, etc.) was not possible, DNA analysis was applied. DNA was isolated from 20 skeletal remains (bone and tooth samples) and six reference samples (blood from living relatives) and amplified using PowerPlex® Fusion and PowerPlex®Y23 kits. DNA profiles were generated for all reference samples and 17 skeletal remains. A statistical analysis (calculation of paternity, maternity, and sibling indexes and matching probabilities) resulted in 10 positive identifications. In this study, 5 individuals were identified based on one reference sample. This has once again demonstrated the significance of DNA analysis in resolving the most complicated cases, such as the identification of commingled human skeletal remains. 相似文献
873.
Isabela G. G. Choi M.Sc. Eduardo F. Duailibi‐Neto Ph.D. Thiago L. Beaini Ph.D. Renan L. B. da Silva D.D.S Israel Chilvarquer Ph.D. 《Journal of forensic sciences》2018,63(3):692-698
Few studies have assessed the use of the frontal sinus cavities for sex differentiation. In this study, a new methodology was used to assess the reliability of CBCT images of the frontal sinus cavity for determining sex based on the sexual dimorphism found in this anatomical structure. The survey sample consisted of 130 scans that were reconstructed three‐dimensionally. 3D images of the cavity volume in the frontal, lateral, and basal views were exported in TIFF. The following variables were measured in a second program: area, perimeter, bounding rectangle, ellipse fit, circularity, aspect ratio, roundness, solidity, and Feret's diameter. The methodology demonstrated the existence of sexual dimorphism with an accuracy of 80.0% in the logistic regression model. The basal view had the greatest explanatory power in the final model. This methodology may be used as an alternative way of determining a reliable biological profile during the analysis of skeletal remains. 相似文献
874.
Laser scanning technology is increasingly being used in forensic anthropological research to obtain virtual data for archival purposes and post hoc measurement collection. This research compared the measurement accuracy of two laser scanners—the FARO Focus3D 330X and the FARO Freestyle3D—against traditionally obtained (i.e., by hand) control data (N = 454). Skeletal data were collected to address a novel question: the ability of laser scanning technology to produce measurements useful for biological characteristic estimation, such as sex and stature. Results indicate that both devices produced measurements very similar to control (c. 3‐mm average absolute error), but also illuminate a tendency to under‐measure. Despite these findings, the virtual data produced sex and stature estimates that varied little from control‐produced estimates, signifying the usefulness of virtual data for preliminary biological identification when the skeletal elements are no longer available for physical analysis. 相似文献
875.
Visual identification is the most common identification method used by medical examiners but is not always possible. Alternative methods include X‐ray, fingerprint, or DNA comparison, but these methods require additional resources. Comparison of serial numbers on implanted medical devices is a rapid and definitive method of identification. To assess the practicality of using this method, we reviewed 608 consecutive forensic autopsies performed at a regional medical examiner office. Of these, 56 cases required an alternative method of identification due to decomposition (n = 35), gunshot wound (n = 9), blunt trauma (n = 6), or charring (n = 6). Of these 56 cases, eight (14.3%) were known to have an implanted medical device. Of these eight cases, five (63%) could be positively identified by comparing serial numbers. If an implanted medical device is known to be present, and medical records are available, identification by medical device serial number should be a first‐line method. 相似文献
876.
Monica M. Warner M.A. Amber M. Plemons M.A. Nicholas P. Herrmann Ph.D. Laura A. Regan Ph.D. 《Journal of forensic sciences》2018,63(2):395-402
Isoscape refinement is an essential component for accurately predicting region‐of‐origin in forensic investigations involving isotope analysis of unidentified human remains. Stable oxygen (δ18O) and hydrogen (δ2H) isotopes were measured from 57 tap water samples collected across Mississippi to model refined isoscapes for the state. A tap water conversion equation, δ18Otw=1.64 δ18Op?31.35, was developed for the southeastern USA to test the prediction accuracy of the δ18Otw isoscape using individuals with known residential histories. A local Mississippi resident (USAFA‐134) was assigned with 90% probability to the correct region‐of‐origin reported by the participant. Assignments for Georgia residents (USAFA‐118 and USAFA‐205) had variable results, predicting USAFA‐118 from Mississippi and USAFA‐205 as a nonlocal resident. Stable isotope values often overlap geographically and a multi‐isotope approach should be used when narrowing region(s)‐of‐origin(s). This study demonstrates the utility of refining isoscapes and the importance of tissue calibration in prediction assignments of human remains. 相似文献
877.
This study tests the effects of corporate social responsibility on employee-company identification (E-C identification) and employee performance, the effects of E-C identification on employee performance, the mediating effects of E-C identification on the relationship between corporate social responsibility and employee performance, and the moderating effects of organizational trust on the relationship between E-C identification and employee performance based on multiple regression analysis with the data collected from 140 employees and their immediate supervisors. The results reveal that corporate social responsibility associations and corporate social responsibility participation are able to influence E-C identification and employee performance; E-C identification is able to influence employee performance; E-C identification fully mediates the relationship between corporate social responsibility associations and corporate social responsibility participation and employee performance, organizational trust moderates the relationship between E-C identification and employee performance. 相似文献
878.
ZHANG Zhong 《浙江省政法管理干部学院学报》2017,31(5):23-31
Evidentiary adjudication is one of the important signs of progress of litigation and judicial civilization. There are three key concepts under the principles of evidentiary adjudication: facts, evidence and fact identification. They form a kind of logic relationship among proposition, the argument and conclusion. As a logical starting point of judicial proof, the case facts have legal determinacy which is different from the facts in philosophical sense, and also different from the fact that as a foundation for the judicial judgment. As the basis of the facts, the evidence emphasizes more the relevance and legitimacy in form. As a logical end point of judicial proof, fact identification is a rational, rigorous proof, but the case facts happened in the past, the fact-finder can only see the evidence, and can only use the evidence to find the facts, which determines the uncertainty of fact identification. 相似文献
879.
Seong Hwan Park Ph.D. M.D. ; Yong Zhang Ph.D. M.D. ; Huguo Piao M.D. ; Dong Ha Yu B.S. ; Hyun Ju Jeong B.S. ; Ga Young Yoo B.S. ; Tae-Ho Jo Ph.D. ; Juck-Joon Hwang Ph.D. M.D. 《Journal of forensic sciences》2009,54(5):1131-1134
Abstract: Calliphorinae fly species are important indicators of the postmortem interval especially during early spring and late fall in Korea. Although nucleotide sequences of various Calliphorinae fly species are available, there has been no research on the cytochrome c oxidase subunit I (COI) nucleotide sequences of Korean Calliphorinae flies. Here, we report the full-length sequences of the COI gene of four Calliphorinae fly species collected in Korea (five individuals of Calliphora vicina , five Calliphora lata , four Triceratopyga calliphoroides and three Aldrichina grahami ). Each COI gene was amplified by polymerase chain reaction and directly sequenced and the resulting nucleotide sequences were aligned and analyzed by MEGA4 software. The results indicate that COI nucleotide sequences can be used to distinguish between these four species. Our phylogenetic result coincides with recent taxonomic views on the subfamily Calliphorinae in that the genera Aldrichina and Triceratopyga are nested within the genus Calliphora . 相似文献
880.
Snjeana Dijan M.Sc. ; Goran uri M.D. ; Dinko Pavlini M.D. ; Mladen Marciki Ph.D. M.D. ; Dragan Primorac Ph.D. M.D. ; Gordan Lauc Ph.D. 《Journal of forensic sciences》2009,54(3):608-609
Abstract: Aiming to estimate the frequency of various types of errors that can occur in the large-scale process of identification, we identified and compared genotypes of 911 parent–child pairs in the database of 3498 relatives of people that disappeared during the 1991/1992 war in Croatia. Genotypes of 891 pairs (97.8%) were matching, while 20 pairs did not match in one or more loci. Reanalysis of these samples revealed that out of 1822 analyzed genotypes, one genotype was completely wrong, and two genotypes had one wrong allele because of human errors. Five genotypes had a single wrong allele due to either polymerase chain reaction or electrophoresis errors. In five genotypes mutations were the cause of mismatch. Genetic inconsistencies with parentage were found in four "fathers" (4.2%) and three "mothers" (0.36%). As the majority of observed single-locus errors were caused by nonhuman errors, all databases produced with similar technology would probably have comparable level of errors. 相似文献