Polymorphism analysis of Y-chromosomal haplotypes in the Chinese Han population living in the Shaanxi province of China

Population: Chinese Han population living in the Shaanxi Province of China.     

论行政审批制度改革后的治安行政管理

治安行政管理工作是公安行政管理的重要组成部分。行政审批制度改革后治安行政管理应从加强行政审批权力的监督制约入手。一是完善审批程序,规范审批条件;二是引入监督机制,搞好审批公开;三是加强与有关部门的协调配合;四是完善行政审批救济程序;五是加强后续监管措施。     

浅谈反垄断法对行政垄断的规制

行政垄断严重危害了市场的公平竞争 ,是市场体制形成和运行的重大障碍。我国必须尽快制定专门的反垄断法 ,采取强有力的措施 ,加大打击行政垄断的力度。     

主诉检察官制度研究

主诉检察官制度 (下称主诉制度 )自推出至今仍然存在理论上的困惑和操作上的难度 ,可以说当前最需要的是对这一制度的基本理论问题作冷静分析及对一些改革举措作理性反思。文章从解决主诉制度的理论分歧着手 ,结合国外的检察制度 ,阐述该制度的理论基点的定位及合理化建构 ,以完善我国的主诉制度     

论我国刑事审判监督制度的缺陷与完善

我国刑事审判监督制度具有认识论、权力制约论、司法公正和人权保障四方面的理论基础。虽然我国刑事审判监督制度仍存在着一定的缺陷,但可以从完善刑事审判活动监督制度和刑事裁判监督制度两方面予以解决。     

PCR-RFLP在mtDNA多态性分析中的应用

目的探讨PCR-RFLP法在mtDNA多态性分析中的应用价值。方法应用限制酶RsaI和MnlI消化mtDNA D-LOOP区（HVI16106～16297）PCR扩增产物,聚丙烯凝胶电泳进行RFLP分型,对不同PCR-RFLP分型扩增产物进行测序,并对150例辽宁汉族随机个体及30例真三联家系血样进行检验。结果在150例随机个体中,RsaI和MnlI酶切分别发现3和8种表型,DNA测序结果和PAGE分型结果一致,GD值分别为0.107,0.670。联合两种酶切,发现12种表型,GD值为0.708。在30个家系中,母子的RFLP带型完全相同。结论 PCR-RFLP法适合在基层实验室mtDNA分析中应用。     

济南汉族群体5个Y-SNP位点的多态性分析

目的 探讨济南汉族群体5个Y-SNP位点的多态性并评价其在法医学中的应用.方法 采用片段长度差异等位基因特异性PCR(fragment length difference allele specific PCR,FLDAS-PCR)对济南汉族群体共103名男性无关个体5个Y-SNP标记(M89、M9、M122、M134...     

Analysis of Dopamine D2 Receptor (DRD2) Gene Polymorphisms in Cannabinoid Addicts*

Abstract: The gene encoding the dopamine D2 receptor (DRD2) has been suggested as a candidate gene for substance dependence. In this study, the possible association between Taq1A and Taq1B DRD2 polymorphisms and cannabinoid dependence was investigated. One hundred and twelve cannabinoid addicted and 130 healthy control subjects were included in this study. The Taq1A and Taq1B genotypes were determined in all subjects by polymerase chain reaction. For each polymorphism (A or B), the subjects were categorized into three groups according to their genotype, that is, the subjects with alleles A1/A1, A1/A2, A2/A2; B1/B1, B1/B2, and B2/B2. A significant association was found between Taq1A gene polymorphism and cannabinoid addicts compared to the control subjects. This finding suggests that polymorphism of the Taq1A, but not the Taq1B, may be associated with the susceptibility to cannabinoid dependence. Further clinical studies are required to be carried out for confirmation and evaluation of these findings.     

线粒体DNA nt8584、nt8701单核苷酸多态性研究

目的建立一种快速、准确的线粒体DNA单核苷酸多态性位点检测方法。方法收集62份无关个体血液样本,应用荧光定量PCR技术研究线粒体DNA nt8584、nt8701位点在中国汉族人群中的多态性。结果 nt8584位点检测到10例nt8584A、52例nt8584G,nt8584A/G变异频率为16∶84;nt8701位点检测到27例nt8701A、35例nt8701G,nt8701A/G变异频率为44∶56。结论所建立的荧光定量PCR方法分型准确、耗时短,适用于法医DNA检验。     

Molecular Identification of Three Indian Snake Species Using Simple PCR–RFLP Method*

Abstract: Three endangered Indian snake species, Python molurus, Naja naja, and Xenochrophis piscator are known to be significantly involved in illegal trade. Effective authentication of species is required to curb this illegal trade. In the absence of morphological features, molecular identification techniques hold promise to address the issue of species identification. We present an effective PCR–restriction fragment length polymorphism method for easy identification of the three endangered snake species, Python molurus, Naja naja, and Xenochrophis piscator. A 431‐bp amplicon from cytochrome b gene was amplified using novel snake‐specific primers following restriction digestion with enzymes Mbo II and Fok I. The species‐specific reference fragment patterns were obtained for the target species, which enabled successful identification of even highly degraded shed skin sample confirming the utility of the technique in case of poor‐quality DNA. The assay could be effectively used for forensic authentication of three Indian snake species and would help strengthen conservation efforts.