放线杆菌外膜蛋白酵母双杂交诱饵质粒的构建及自激活与毒性检测

为了筛选胸膜肺炎放线杆菌外膜蛋白在肺泡上皮细胞上的作用受体,应用酵母双杂交技术构建诱饵质粒载体pGBKT7-omp(胸膜肺炎放线杆菌外膜蛋白),从胸膜肺炎放线杆菌提取基因组DNA,用PCR方法获得omp,克隆于pGBKT7载体上,进行酶切鉴定及序列分析,并检测pGBKT7-omp在酵母双杂交系统中的自激活和毒性作用。结果表明,成功构建了诱饵质粒载体pGBKT7-omp,并证明其在酵母双杂交系统中无自激活及毒性作用。表明pGBKT7-omp可应用在酵母双杂交系统中,用于寻找猪肺泡上皮细胞cDNA文库中与omp相互作用的蛋白质。     

Feigning Hand Preference? A Case Report Preliminary Data

Hand preference may be crucial in the forensic domain, notably in cases where the assailant is known to be left‐handed and the defendant claims to be right‐handed (or vice versa). In such cases, forensic psychologists or physicians may be asked to test the hand preference of the defendant. However, hand preference may be faked. The case described here illustrates this problem and addresses potential solutions. We also present preliminary data showing that a standard instrument for measuring handedness is sensitive to feigning. We conclude that when hand preference is determined, multiple sources of information should be assessed in order to identify possible feigning.     

Case series of completed suicides by burning over a 13-year period

Suicide by burning is an extreme act that is uncommon in the United States and throughout the Western world. The characteristics of people who complete such acts are not well understood. To address this issue, we examined the death records of the King County Medical Examiner's Office in Washington State over the 13 years from 1996 to 2009. Twenty-five cases of suicide by burning were identified and used to characterize decedent demographics, circumstances of death, and motivating factors. Compared to other methods of suicide, burning demonstrated a significant overrepresentation of decedents who were women, 40-59 years of age, and Asian/Pacific Islander. They also tended to have previous psychiatric illness and/or substance abuse issues. Self-burning predominantly occurred at the decedent's residence with the intent of suicide given. There was no unifying theme in motivating factors. Together, these data represent the characteristics of people whose death is because of suicide by burning in King County, Washington.     

The law and the sublime: Rethinking the self and its boundaries

Christine Battersby has argued that it is Kant (and not Descartes) who provides the paradigm model of what it is to be a self in modernity. The Kantian self is established in opposition to its other. The body is commonly envisaged as a container, with selfhood as something that is defended against the outside. In contrast, she proposes a feminist reworking of such a model of selfhood, applicable to both men and women, in which the self and other emerge over time through patterns of relationality. This paper introduces Battersby’s work by focusing upon her early analysis of Kantian aesthetics, in particular the sublime. The aim is to draw out some of the legal and political implications of her work, particularly with regard to the common law’s developing conception of privacy. This is carried out by distinguishing her ontological position from the psychology of Carol Gilligan and then by considering the overlapping concerns of Jennifer Nedelsky in the area of legal theory.

THE SELF‐REPRESENTED LITIGANT: IMPLICATIONS FOR THE BENCH AND BAR

It is widely accepted that the number of self‐represented litigants has skyrocketed nationwide, especially in family law cases. Although nationwide comprehensive data on the number of self‐represented litigants do not exist, anecdotal evidence supports the belief that self‐representation is increasing. The challenge for courts and the entire legal profession is how to respond. Most observers in Indiana would agree that the traditional model of family law litigation—both spouses represented by lawyers settling their disputes before a judge—is no longer the norm in family law cases. Judges face a dilemma: assisting a self‐represented litigant to level the playing field against a represented party is seen by many as violating impartiality, even if the assistance is rendered to create a just result. In an effort to address the situation, the Indiana Supreme Court created the Pro Se Advisory Committee in April 2001. This article explores the long‐range implications of the issue of self‐represented litigants on Indiana's court system in hope that it will provide some insight for other jurisdictions. The first part of the article addresses the numbers of self‐represented litigants by tracking growth or declines in self‐represented cases and assessing whether there are any pockets of self‐represented litigants geographically or in certain case types. The second part of the article puts Indiana into context with the rest of the nation and reviews national trends. The third section reviews Indiana's response to self‐represented litigants over the last decade. The fourth section reviews current and ongoing projects in Indiana. The article concludes that the issue of self‐represented litigants will not fade away and that the challenge that guides the legal profession is how we provide equal access to justice for all who enter our courthouses.     

Study on the Method Used to Display Self‐fading Lines and Erasable Lines

Two new kinds of writing tools are popular in China's market. One is a self‐fading pen, and another is an erasable pen. The ink of the two kinds of writing tools has a remarkable characteristic that it can gradually fade or disappear under heat or be rubbed off. How to reveal the disappeared written lines is a very important question for document examiners. In this article, three series of ink line samples were made with five types of self‐fading pens, 18 types of erasable pens, and three types of papers. Temperature, humidity, and lighting are known as influential factors of the process, and the effect of fading was examined. Luminescence, ultraviolet (UV), sidelight, electrostatic indentation development,low temperature, and solution revealing methods are found to be effective methods used to reveal the disappeared written lines. The best operating conditions for each method were obtained from the conducted experiments.     

道路自信、理论自信、制度自信的基本内涵浅析

党的十八大报告提出,全党面对党和人民90多年奋斗、创造、积累的根本成就时,要坚定“道路自信”、“理论自信”、“制度自信”。只有在坚定“三个自信”的同时,脚踏实地、扎扎实实地干好自己的事业,我们的道路自信才不是固步自封,我们的理论自信才不是夜郎自大,我们的制度自信才不是骄傲自满。有了这样的底气和精神状态,我们就一定能完成党确定的宏伟目标。     

A Discussion of the Incongruence Between Optimism and Academic Performance and Its Influence on School Violence

Abstract

This research suggests that incongruence between how a child views self and the aspirations pursued can lead to maladaptive externalizing behaviors in the form of activities related to school violence. As such, the purpose of this research is to examine the degree to which negative behaviors exhibited in adolescents are influenced by various degrees of optimism. This research approaches the issue of school violence with great care and because of the broad definitional issues concerning school violence and the nature of that data used in the study the researchers have chosen to reference the concept of school violence as “activities related to school violence.”     

成效、问题及对策：山东省两县市“多村一社区”的社区服务体系建设的调查

该文分析了通过撤村建社区,在中心村设立社区服务中心,构建起政府性公共服务体系、社区自我服务体系与市场化服务体系有机结合的农村社区服务体系的成效,即政府性公共服务中有的服务项目规范化、专业化程度较高,村民满意度比较高;社区与企业合作提供的便民利民服务的供给比较充分。但是,也存在诸如县域间社区服务中心的建设力度差距较大、社区村（居）民自我服务匮乏、中心村与非中心村服务资源获得存在较大差距等问题。为此建议,通过强化各级政府财政转移支付,促进社区基础公共服务设施均等化配置;持续推进村（居）民自治与社区参与,促进“多村一社区”建设与村（居）民自治二者间的协调发展;加强非中心村的服务站点建设,缩小社区服务资源获得方面的差异等途径以促进“多村一社区”的社区服务体系建设。