Efficiency Analysis of EX16 +10Y Kit on Detection of the Uygur Population in Xinjiang ProvinceCSCD

Objective: To analyse the efficiency of EX16+10Y kit on the forensic detection of the Uygur in Xinjiang province. Methods: The blood samples were extracted from 4 620 male individuals of Uygur in Xinjiang province, and amplified by EX16+10Y kit. The typing of amplification products was performed by 3130xl genetic analyzer. Results: The genotyping graphs of 15 autosomal STR loci and 10 Y -chromosomal STR loci from 4 620 male individuals of Uygur in Xinjiang province were acquired completely. The genotype distribution of 15 autosomal STR loci was consistent with Hardy-Weinberg equilibrium. The heterozygosity, polymorphism information content and discrimination power of STR loci were 0.637-0.838, 0.580-0.860 and 0.811-0.978, respectively. There were 766 haplotypes in 10 Y -chromosomal STR loci. Conclusion: The test results of EX16+10Y kit is accurate and trustworthy, which can simultaneously be used for the individual identification and the screening of paternal pedigree in practical work. © 2018 by the Editorial Department of Journal of Forensic Medicine.     

复方苦参注射液生物碱类成分及血中移行成分的分析

目的 利用超高效液相色谱—电喷雾离子化—四极杆飞行时间质谱（ultra-high-performance liquid chromatography-electrospray ionization-quadrupole time-of-flight mass spectrometry,UHPLC-ESI-QTOF/MS）结合UNIFI构建靶向筛查策略分析复方苦参注射液（Compound Kushen Injection,CKI）的生物碱类成分及血中移行成分。方法 首先,采用ESI-QTOF/MS的全信息串联质谱技术采集经UHPLC分离的CKI样品及其空白样品、CKI给药后的大鼠血浆样品及空白血浆样品的信息;然后,建立CKI生物碱化学成分数据库,与MSE数据一同导入UNIFI进行靶向筛查;依据母离子、碎片离子的精确质量对筛查出的化合物进行识别;最后,使用MassLynx 4.1工作站对UNIFI输出的结果进行核实。结果 本实验共分离、鉴定了20种CKI生物碱,其中17种生物碱能在大鼠血浆中被检出。结论 该方法能够快速分析CKI生物碱类成分及其血中移行成分,可为CKI的质量控制及药效物质研究提供参考。