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DNase-Ⅰ纯化结合碱性裂解法提取混合斑精子DNA 总被引:1,自引:0,他引:1
目的研究脱氧核糖核酸酶I(DNase-I)纯化结合碱性裂解法提取混合斑精子DNA的方法在法医学中的应用。方法收集79份性犯罪案件混合斑检材,分别用DNase-I纯化结合碱性裂解法和差异裂解法提取精子DNA,采用STR荧光标记复合扩增体系进行16个STR基因座分型,并比较检验结果。结果应用DNase-I纯化结合碱性裂解法提取精子DNA,64例检材分型成功;应用差异裂解法提取精子DNA,57例检材分型成功;两种方法比较结果存在显著性差异(P=0.039),DNase-1纯化结合碱性裂解法提取精子DNA的STR分型成功率更高,成本低廉。结论DNase-I纯化结合碱性裂解法提取混合斑精子DNA可提高检验成功率,操作简便,快速,易于自动化,适于法医学个体识别鉴定。 相似文献
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Mark TurnerAuthor Vitae 《Computer Law & Security Report》2009,25(1):101-105
This column provides a concise alerting service of important national developments in key European countries. Part of its purpose is to compliment the Journal's feature articles and Briefing Notes by keeping readers abreast of what is currently happening “on the ground” at a national level in implementing EU level legislation and international conventions and treaties. Where an item of European National News is of particular significance, CLSR may also cover it in more detail in the current or a subsequent edition. 相似文献
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Besfat Dejen Engdaw 《国际公共行政管理杂志》2020,43(7):644-654
ABSTRACTThis study is conducted with the general objective of assessing the impact of quality public service delivery on customer satisfaction in Ginbot20 sub-city. To conduct the study correlational research design, and quantitative research approach have been employed. A total of 88 samples both from customers (68) and service providers (20) were selected using simple random sampling and systematic random sampling techniques respectively. Data were collected using questionnaires. Based on the data collected, the study comes up with certain findings. Among the major findings, the perception of customers and service providers on quality service delivery and customer satisfaction is different, responsiveness, tangibility, assurance, and reliability have positively associated with customer satisfaction. However, empathy has negatively associated with customer satisfaction. Generally, the likelihood of customers gets satisfied when there is quality service delivery is 0.84 (84%) while get satisfied with the probability value of 0.038948(3.8948%) in the absence of quality service delivery. 相似文献
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通知删除规则率先确立于《信息网络传播权保护条例》,《侵权责任法》互联网条款将其扩展为通知加采取必要措施,并使其广泛适用于一般网络侵权领域。《电子商务法》知识产权保护条款又有相应的规定。随着互联网技术和商业模式迅速发展,新类型网络服务层出不穷,在适用通知删除等规则中不断产生新问题和新争议。除另有特别规定外,新类型网络服务应当适用《侵权责任法》第36条规定的通知加采取必要措施,其中必要措施的界定具有开放性和灵活性,必要措施的妥当性取决于与特定网络服务的匹配性,可以根据网络服务提供者的具体情形,以利益衡量的方式确定相应的具体措施。个案中应当根据新类型网络服务的特性以及产业发展需求等情况,合理权衡特定网络服务与必要措施的适应性,妥善确定应当采取的必要措施。 相似文献
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浅析网络平台服务商的名誉权侵权责任 总被引:1,自引:0,他引:1
杨海静 《山西省政法管理干部学院学报》2009,22(4):53-56
网络的匿名特点使人们可以自由地发表言论而不会轻易被发现,民事主体的名誉权在网络环境下显得异常脆弱,越来越多的网络服务提供商卷入了侵权诉讼中。其中网络平台服务商应否对第三人的诽谤行为承担责任成为网络环境下亟待解决的问题。综合考量世界各国的立法和实践,结合我国的实际,在界定网络平台服务商责任时应以最低程度的注意义务和过错责任为宜。 相似文献
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Bienvenue JM Duncalf N Marchiarullo D Ferrance JP Landers JP 《Journal of forensic sciences》2006,51(2):266-273
The current backlog of casework is among the most significant challenges facing crime laboratories at this time. While the development of next-generation microchip-based technology for expedited forensic casework analysis offers one solution to this problem, this will require the adaptation of manual, large-volume, benchtop chemistry to small volume microfluidic devices. Analysis of evidentiary materials from rape kits where semen or sperm cells are commonly found represents a unique set of challenges for on-chip cell lysis and DNA extraction that must be addressed for successful application. The work presented here details the development of a microdevice capable of DNA extraction directly from sperm cells for application to the analysis of sexual assault evidence. A variety of chemical lysing agents are assessed for inclusion in the extraction protocol and a method for DNA purification from sperm cells is described. Suitability of the extracted DNA for short tandem repeat (STR) analysis is assessed and genetic profiles shown. Finally, on-chip cell lysis methods are evaluated, with results from fluorescence visualization of cell rupture and DNA extraction from an integrated cell lysis and purification with subsequent STR amplification presented. A method for on-chip cell lysis and DNA purification is described, with considerations toward inclusion in an integrated microdevice capable of both differential cell sorting and DNA extraction. The results of this work demonstrate the feasibility of incorporating microchip-based cell lysis and DNA extraction into forensic casework analysis. 相似文献
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论网络侵权责任中的反通知及效果 总被引:1,自引:0,他引:1
随着互联网科技的不断发展,人们日常生活对网络的依赖有增无减。人们在享受网络带给生活的巨大便利的同时,也倍受各种网络侵权行为的干扰。《侵权责任法》第36条为解决网络侵权问题找到了出口,但除了该条第2款中明确规定的被侵权人享有向网络服务提供者发出通知的权利之外,侵权网络用户也享有向网络服务提供者发出反通知的权利。这样才能平衡网络侵权中以网络服务提供者为中心的被侵权人、侵权网络用户和其他网络用户之间的利益。 相似文献
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网络服务提供者的侵权责任——以《侵权责任法》第36条第2款、第3款为中心 总被引:1,自引:0,他引:1
周强 《北京政法职业学院学报》2011,(1):43-48
《侵权责任法》第36条第2款、第3款对网络用户直接侵权时,网络服务提供者承担连带责任的情形进行了规范。但这两款内容过于原则,需要运用解释论的方法予以细化。第36条关于网络服务提供者侵权责任的规定要在权利人、社会公众、网络服务提供者之间达到更为合理的平衡,有待将来的司法解释进行完善。 相似文献