番鸭细小病毒MDPV YZ株VP2和VP3蛋白基因的克隆和序列测定

根据国外已发表的番鸭细小病毒(MDPV)FM株基因组核苷酸序列设计了1对引物,应用PCR技术扩增了MDPV YZ株的VP2和VP3蛋白基因片段.将扩增后的VP2和VP3蛋白基因克隆到pCR 3.1 T载体上,MDPV YZ株基因大小为1764 bp,编码528个氨基酸,并对插入片段进行序列测定.测定结果表明,我国分离的MDPV YZ株蛋白基因序列与国外发表的序列的同源性为98.5%.     

高校毕业生就业结构性失衡及应对

近年来,结构性失衡已成为高校毕业生就业的主要矛盾,突出表现在市场供需不匹配、区域结构性矛盾、公共就业服务供需矛盾、性别差异等方面.其根源主要在于产业转型升级创造高端岗位的速度远低于高校毕业生的增速,区域性发展不平衡,以及专业设置的刚性和传统思想根深蒂固等.研究认为,可以采取协同推进产业转型、建立市场导向的专业设置机制、...     

Is Bigger Really Better? A Comparative Analysis of Municipal Mergers in Australian and Japanese Local Government

While structural reform by means of municipal mergers has been employed in many different countries, council consolidation has been especially marked in Australian and Japanese local government. This suggests that much can be learned by examining amalgamation through the analytical prism of comparative analysis. In this article, we investigate structural reform through municipal amalgamation in Australian and Japanese local government by examining the aims of amalgamation, the methods employed by public policy makers and the outcomes which were achieved. From this comparative analysis, we attempt to draw some broader lessons for local government policy making aimed at local government reform.     

The Unintended Consequences of Structural Change: When Formal and Informal Institutions Collide in Efforts to Address Wicked Problems

Significant restructuring of bureaucracies has occurred to facilitate joined-up working. This article draws on new institutionalism to explore the rationale behind the use of structural change for the promotion of joined-up working. It argues that a strong institutionalized myth has emerged which has created isomorphic pressures in the public sector to instigate structural changes in the name of vertical integration. These combine with informal institutions in ways that can be both productive and unproductive, highlighting the need to find a balance between formal institutional change and informal institutionalized practices, which often go unacknowledged as a powerful influence on policy.     

转走银行客户资金型票据诈骗过程分析

转走银行客户资金型票据诈骗是指票据诈骗犯罪嫌疑人以银行客户资金为侵害对象,利用票据诈骗手段将该资金非法占有的犯罪行为。在银行与企业双方都存在漏洞时,银行内外人员勾结,伪造或盗用财务专用章,使企业的资金被转走。银行票据诈骗案之所以“成功”发生,与银行票据业务涉及环节多、监督管理过程长以及其中可能出现的漏洞较多有关。     

政务信息公开满意度影响因素分析——以政府网站为例

以政府网络为视角,研究政务信息公开满意度影响因素,有助于政府部门改进信息化服务工作,提高公众满意度.本文通过对美国顾客满意度指数模型(A CSI)进行改进并参考欧洲顾客满意度指数模型(ECSI),构建了我国政府网站政务信息公开满意度模型,运用问卷调查和结构方程模型(SEM)对所构建模型进行实证检验,从公众视角揭示我国政...     

Isolation and identification of an isomeric sildenafil analogue as an adulterant in an instant coffee premix

The proliferation of adulterated health foods and beverages in the market demands a comprehensive analytical strategy to identify the adulterants, particularly those of isomeric phosphodiesterase 5 (PDE5) inhibitors. An instant coffee premix (ICP) purchased from an online retailer was flagged for suspected adulteration through PDE5 inhibition assay. The ICP was then analysed using suspected-target and non-targeted screenings of a liquid chromatography-quadrupole time-of-flight mass spectrometry. Based on these findings, a PDE5 inhibitor initially assigned as compound X was isolated from the ICP by employing a liquid chromatography-diode array detection before its structural elucidation with liquid chromatography-ultraviolet (LC-UV) spectroscopy and nuclear magnetic resonance (NMR) spectroscopy. The suspected-target screening matched the protonated molecule ([M + H]⁺) precursor ion of compound X at m/z 499.2310 with two suspected analytes that are structural isomers of one another. The fragmentation patterns of compound X were comparable to those analogues in the dithiocarbodenafil group through the non-targeted screening. These findings, complemented by the LC-UV and NMR spectroscopy data, together with the chromatographic separation of related structural isomers, conclude the identity of compound X. To our best knowledge, this is the first study to report the presence of 3,5-dimethylpiperazinyl-dithiodesmethylcarbodenafil in an ICP sample.

Key points

• The herbal-based male sexual performance products’ lucrative market has instigated their rampant adulteration, particularly with PDE5 inhibitors.
• The adulterated products may also contain analogues of the approved PDE5 inhibitors, which usually passed into the market undetected as they are not included in the routine targeted screening procedure.
• The present study detected, isolated, and identified an isomeric sildenafil analogue from an instant coffee premix sample using rapid qualitative assay and comprehensive analytical analysis.
• This paper highlighted the applicability of the established strategies for routine casework, particularly in a forensic drug testing laboratory.

     

Getting Prepared for the Asia Region Funds Passport

正Financial cooperation is essential to the integration of Asian economies.To reinforce such cooperation,the key is to coordinate existing financial resources in Asia.Therefore,expanding the scope of the Asia Region Funds Passport(ARFP)is an important step[Note:The Asia Region Funds Passport is an initiative put forth at the APEC Finance Ministers’meeting held in September 2013 in Bali,Indonesia].Recently,progress has been made in the mutual recognition of regional funds     

科研经费试点“包干制”探析

文章以党的十九届四中全会精神为指导,从制度创新视角研究科研经费管理问题,探析深化"放管服"改革下科研经费试点"包干制"的背景现状、原因和问题,旨在推动科研经费管理制度变革,把包干制度创新精神和优势转化为治理效能,更好释放科研活力,服务创新型国家建设。     

To survive is all: How the authoritarian core tackles neighboring dictatorships

This article attempts to answer why autocrats of illiberal regional powers intervene in the politics of neighboring dictatorships and argues that the dictator always prioritizes his survival and thus intervenes if he perceives his survival to be under direct threat. The formal model proposes three factors that determine the level of perceived threat: demographic composition, structural similarity, and regime dynamic of autocratic countries. The authoritarian core must pay close attention to those neighboring autocratic countries that are suffering from regime change, are close to its own densely populated region, and have regime types or social structure similar to itself. Additionally, if hostile ethnic or religious groups are highly concentrated in some areas of the authoritarian core where it borders autocracies experiencing regime instability, the authoritarian core will be motivated to intervene in the domestic affairs of those neighboring countries. Using QCA and case studies, this article confirmed that whether an authoritarian core will take action against countries in geographical proximity depends on a combination of these three factors.