全文获取类型
| 收费全文 | 92篇 |
| 免费 | 2篇 |
专业分类
| 工人农民 | 2篇 |
| 世界政治 | 3篇 |
| 外交国际关系 | 6篇 |
| 法律 | 73篇 |
| 中国政治 | 4篇 |
| 政治理论 | 2篇 |
| 综合类 | 4篇 |
出版年
| 2024年 | 3篇 |
| 2023年 | 3篇 |
| 2022年 | 1篇 |
| 2021年 | 2篇 |
| 2020年 | 3篇 |
| 2019年 | 3篇 |
| 2018年 | 2篇 |
| 2017年 | 3篇 |
| 2016年 | 6篇 |
| 2015年 | 1篇 |
| 2014年 | 11篇 |
| 2013年 | 13篇 |
| 2012年 | 6篇 |
| 2011年 | 4篇 |
| 2010年 | 6篇 |
| 2009年 | 4篇 |
| 2008年 | 5篇 |
| 2007年 | 5篇 |
| 2006年 | 7篇 |
| 2004年 | 2篇 |
| 2001年 | 1篇 |
| 2000年 | 1篇 |
| 1995年 | 1篇 |
| 1985年 | 1篇 |
排序方式： 共有94条查询结果，搜索用时 15 毫秒
51.
52.
马立克病肿瘤相关抗原间接ELISA检测方法的建立 总被引：1，自引：0，他引：1
用自制并纯化的马立克病肿瘤相关表面抗原(MATSA)免疫SPF级BALB／c小鼠制备阳性对照抗体,以SPF级BALB／c小鼠血清为阴性对照抗体,筛选间接ELISA检测MATSA抗体的最佳反应条件。结果,包被液为0．1 mol／L pH 9．6的碳酸盐缓冲液,抗原包被浓度为4μg／mL,封闭液为含10 g／L BSA的PBST,抗血清稀释比例为1:800,酶标抗体稀释比例为1:1200,酶标抗体作用时间为37℃温育45 min,底物作用时间为37℃温育15 min,终止液为2 mol／L H2SO4,洗涤4次,每次1min为最佳反应条件。将建立的方法应用于MATSA单克隆抗体研制过程中阳性克隆的筛选,成功获得了MATSA的单克隆抗体,表明该优化条件为间接ELISA检测MATSA抗体的最佳反应条件。 相似文献
53.
化学显色法快速筛选饮料及尿液中γ-羟基丁酸和γ-丁内酯 总被引：1，自引：0，他引：1
目的建立化学显色法快速筛选饮料及尿液中γ-羟基丁酸(GHB)及其前体γ-丁内酯(GBL)的方法。方法在酸性条件下GHB转化为GBL,GBL和盐酸羟胺在碱性条件下生成γ-羟基丁酰羟胺,γ-羟基丁酰羟胺在酸性条件下和三氯化铁反应,生成紫红色的络合物。结果饮料中GHB最低检出浓度为0.5～2mg/mL,低于常见滥用质量浓度。该方法也可以用于尿液分析,最低检出质量浓度为0.5mg/mL。考察了常见有机溶剂和麻醉镇静药物的干扰。结论该方法简单、安全、快速,为临床和法庭科学实验室快速筛选GHB和GBL提供了便利。 相似文献
54.
55.
Michael S. Martin Shannon K. Dorken Alexander I.F. Simpson Kwame McKenzie Ian Colman 《The journal of forensic psychiatry & psychology》2014,25(6):733-747
The Depression Hopelessness Suicide Screening Form (DHS) includes 12 ‘critical items’, which have not been validated for the prospective prediction of self-harm. We conducted a retrospective cohort study (N = 4196) to validate the ability of the DHS critical items to prospectively predict inmates with at least one incident of self-harm during the first six months of imprisonment. While the critical items were highly sensitive (89.5%) at predicting incidents of self-harm, 51.3% of the inmates endorsed at least one item. Five items reflecting more recent and specific risk factors reduced the referral rate to 17.7%, while maintaining high sensitivity (84.2%). While the DHS has high sensitivity to predict inmates at risk of self-harm, treating all items as equally critical results in excessive numbers of false positives that likely exceed the capacity of prison resources for professional assessment and intervention. Referral rules based on recency and specificity of risk factors are proposed. 相似文献
56.
Crystal Simson Oechsle M.S. Sandra Haddad Ph.D. Joanne B. Sgueglia B.A. Catherine M. Grgicak Ph.D. 《Journal of forensic sciences》2014,59(1):199-207
Biological fluid identification is an important facet of evidence examination in forensic laboratories worldwide. While identifying bodily fluids may provide insight into which downstream DNA methods to employ, these screening techniques consume a vital portion of the available evidence, are usually qualitative, and rely on visual interpretation. In contrast, qPCR yields information regarding the amount and proportion of amplifiable genetic material. In this study, dilution series of either semen or male saliva were prepared in either buffer or female blood. The samples were subjected to both lateral flow immunochromatographic test strips and qPCR analysis. Analytical figures of merit—including sensitivity, minimum distinguishable signal (MDS) and limit of detection (LOD)—were calculated and compared between methods. By applying the theory of the propagation of random errors, LODs were determined to be 0.05 μL of saliva for the RSID? Saliva cards, 0.03 μL of saliva for Quantifiler® Duo, and 0.001 μL of semen for Quantifiler® Duo. In conclusion, quantitative PCR was deemed a viable and effective screening method for subsequent DNA profiling due to its stability in different matrices, sensitivity, and low limits of detection. 相似文献
57.
Chioma Ajoku 《Family Court Review》2023,61(3):483-489
This article touches upon theories accounting for the rise in adolescent anxiety and depression and how the prevalence of adolescent anxiety and depression affects how we navigate parenting plan evaluations. This article also discusses the importance of screening youth for depressive and anxious symptoms and assessing family, parental, and youth risk and protective factors related to adolescent depression and anxiety. This article also highlights the importance of assessing for mediating factors that increase the risk of anxiety and depression in youth in the context of parental separation, such as interparental conflict, financial difficulties, and limited contact with one parent. This article also identifies various methods of assessing depression and anxiety, including the adolescent interview, observations during the interview, and self-report measures. Additionally, this article discusses potential interventions that address mediators of the impact of divorce, such as parenting problems, family conflict, or the parent–child relationship. 相似文献
58.
目的筛选阴影带出现率低且多态性较高的牛四核苷酸STR基因座。方法用Tandem repeats finder软件搜索牛基因组中的四核苷酸重复STR序列片段,用Primer Premier 5.0软件设计引物,然后扩增、电泳,筛选出符合要求的STR基因座,并对100份无关黄牛个体血样进行STR基因座分型。结果共筛选出6个具有多态性的牛四核苷酸重复STR基因座（B006、B007、B008、B022、B025、B026）,其100份无关黄牛个体血样的累积个体识别率和累积非父排除概率分别为0.99995和0.859591。结论本研究筛选出的6个四核苷酸STR基因座阴影带出现率低且多态性较高,可用于牛的个体识别和亲子鉴定的研究。 相似文献
59.
目的 揭示黄精调控DNA拓扑异构酶Ⅱ-α(topoisomeraseⅡalpha, TOP2A)抗胃癌的分子机制。 方法 首先借助“limma”R包对来源于癌症基因组中胃癌与正常组织的转录组数据进行差异表达基因筛选;然后采用加权基因共表达网络分析、CytoHubba的度值和邻域组件中心性算法筛选Hub基因,并基于Hub基因对黄精中抗胃癌成分进行虚拟筛选;最后应用动力学模拟对筛选出来的关键化合物和靶基因相互作用关系进行验证,并揭示其抗胃癌的分子机制。 结果 共鉴定出382个差异表达基因和6个Hub基因,即TOP2A、CDK1、AURKB、BUB1、CCNA2和MCM4;虚拟筛选发现黄精活性成分薯蓣皂苷元与TOP2A结合能力较好;分子动力学模拟证实,薯蓣皂苷元可稳定结合并抑制TOP2A的表达,验证了虚拟筛选结果的准确性;生物学功能富集发现TOP2A主要干预细胞周期通路、p53信号通路和FoxO信号通路。 结论 黄精主要通过薯蓣皂苷元调控TOP2A并作用于细胞周期通路、p53信号通路和FoxO信号通路而达到抗胃癌的作用。 相似文献
60.
利用基于AT指令的DTU,通过GPRS无线通信网络开发了集中式警用移动数据通信系统。在VB6．0下利用Mscomm控件设计了智能通信中心管理软件,可实现智能拨号、对来电号码的追踪、对来电历史记录的查询、对备案数据库以外的来电屏蔽、智能联网、对特密专线拨号以及对特密专线的限权修改等功能。 相似文献