冠心病痰瘀辨证与外周血单核细胞PDGF-A mRNA表达的关系

目的 :观察冠心病“痰瘀”辨证与外周血单核细胞 (PBMC)血小板衍化生长因子A(plateletderivedgrowthfactorA ,PDGF A)mRNA表达的内在联系。方法 :冠心病病人 30例 ,随机分为 3组 ,非痰非瘀组、痰凝心脉组、痰瘀痹阻组 ,每组 10例 ;另有健康志愿者 10例。采用逆转录 聚合酶链反应 (RT PCR)技术 ,检测PBMCPDGF AmRNA的表达。结果 :痰凝心脉组、痰瘀痹阻组PDGF AmRNA表达水平与健康对照组比较 ,差异有显著性 (P <0 .0 5 ) ;痰瘀痹阻组与非痰非瘀组比较 ,差异也有显著性 (P <0 .0 1)。结论 :PBMC中PDGF AmRNA表达的异常可能是冠心病“痰瘀”演变的重要机制之一。     

猪生殖与呼吸综合征病毒N基因的扩增与克隆

应用RT PCR方法扩增出猪生殖与呼吸综合征病毒 (PRRSV)的核衣壳蛋白基因 (N基因 ) ,并将其克隆到 pET 32a载体 ,构建了高效原核表达载体pETN。将 pETN重组质粒转化BL2 1(DE3)宿主菌后 ,对插入片段进行了序列测定及同源性分析。结果表明 ,插入片段序列与PRRSV美洲型ATCCVR2 332株的ORF7核苷酸序列同源性达 99.9% ,与分离毒株的同源性达 99.0 %。     

肺气虚证大鼠血液流变性指标与内皮素、血栓素水平的相关性研究

目的 :探讨肺气虚证大鼠的血液流变性变化与内皮素 (ET)、血栓素 (TXB2 )变化的相关性。方法 :将 6 0只大鼠随机分为模型组与对照组 ,复制肺气虚证模型 ,分别检测血液流变性指标和ET、TXB2 水平。结果 :模型组与对照组比较 ,全血黏度 (高切、低切 )、血浆黏度、红细胞比容、红细胞聚集指数及纤维蛋白原均升高 ,差异有显著性 (P <0 .0 1或P <0 .0 5 ) ;模型组ET和TXB2 明显升高 ,与对照组相比 ,差异有显著性 (P <0 .0 1)。模型组全血黏度与ET、TXB2 呈正相关 (r =0 .95 7,P <0 .0 5 ;r =0 .992 ,P <0 .0 1)。结论 :肺气虚大鼠存在“血瘀”现象 ,且血浆中的ET、TXB2 含量随肺气虚证逐渐加重而呈上升趋势。     

普乐林注射液合辨证治疗缺血性脑卒中临床观察

目的 :观察普乐林注射液合辨证治疗缺血性脑卒中的疗效。方法 :5 6例缺血性脑卒中患者分为治疗组 (30例 )和对照组 (2 6例 ) ,治疗组采用普乐林注射液合辨证治疗 ,对照组采用进口脑活素加辨证治疗。疗程均为 14d。结果 :治疗组显效率达 80 .0 % ,对照组显效率为 6 1.5 4% ,两组显效率比较 ,差异无显著性 (P >0 .0 5 ) ;但治疗组在消除眩晕、肢体麻木方面优于对照组 (P <0 .0 5 )。结论 :普乐林注射液合辨证治疗对缺血性脑卒中有良好疗效     

非典危机与突发公共卫生事件应急法律制度

“急事”呼唤“急法”,国务院《突发公共卫生事件应急条例》的紧急出台是因非典危机而起。本文通过研究这部非常时期的非常立法的背景、目的、指导思想和主要内容,论述了我国建立应急法律制度的现实价值与深远意义。特别强调,非典既是一种呼吸综合症,它所引发的更是一种社会综合症,法律正是通过对社会病的疗治而对付自然病;有了急事,就要有急的意识、急的行为、急的规范,就要依法应急。     

REJOINDER TO GARDNER'S "COMMENTARY ON KELLY AND JOHNSTON'S 'THE ALIENATED CHILD: A REFORMULATION OF PARENTAL ALIENATION SYNDROME'"

In this reply to Richard Gardner, we outline our points of disagreement with his formulation of parental alienation syndrome (PAS), showing that his focus on the alienating parent as the primary cause of children's negative attitudes and rejecting behavior toward the other parent is overly simplistic and not supported by findings from recent empirical research. It follows that we strongly object to Gardner's recommendations for legal and mental health interventions with alienated children as well as the use of the term PAS when referring to this problem.     

Tyrosine Hydroxylase TH01 9.3 Allele in the Occurrence of Sudden Infant Death Syndrome in Swiss Caucasians

Catecholamines, especially noradrenalin, are essential in the control of respiration and arousal. Thus, an impaired production of these neurotransmitters may contribute to the occurrence of sudden infant death syndrome (SIDS). The first step of the noradrenergic synthesis pathway is catalyzed by the enzyme tyrosine hydroxylase (TH). The TH‐encoding gene contains a tetrameric short tandem repeat in intron 1 (TH01), with allele 9.3 reported to be associated with SIDS in German infants. We investigated the allelic frequency of the TH01 marker in 171 Swiss SIDS infants and 500 healthy and gender‐matched Caucasian adults. In our study population, the allelic frequency of the 9.3 allele is similarly distributed in SIDS cases and controls (27.2% vs. 25.6%; p‐value = 0.562). Nevertheless, the TH‐encoding gene is only one of several genes involved in the noradrenergic biosynthesis pathway. Therefore, further genetic investigations are required with focus on the whole noradrenergic signaling system.     

摇晃婴儿综合征争议及研究进展

摇晃婴儿综合征是常见的虐待性颅脑损伤,其病理表现为三联征:脑实质病变、硬脑膜下出血、视网膜出血。虽然摇晃婴儿综合征被广泛运用于医学及法律领域,但是由于目前尚无直接证据证实成人摇晃儿童能造成严重脑损伤甚至死亡的后果,因此摇晃婴儿综合征存在学术争议。争议内容包括摇晃婴儿综合征的生物力学机制、病理生理机制等方面。由于某些疾病也能导致病理三联征,因此在诊断摇晃婴儿综合征过程中,应当注意外伤史、案情调查等。     

What is she doing here? Klinefelter syndrome in forensic casework

In this case report, we describe a sexual assault incident in which the male victim’s seminal fluid contained no sperm cells, as indicated by sperm cell staining and microscopic screening, and DNA profiling results from the non-sperm cell fraction showed a major/minor DNA mixture that could be interpreted as female and male. DNA profiling of a sample from a disposable drinking cup used by the victim at the crime scene provided a single source profile, and showed a 2:1 imbalance between the heights of the X and Y chromosomes, respectively. The victim’s DNA reference sample showed a similar imbalance of the X and Y chromosomes. These observations suggested that the victim might suffer from Klinefelter syndrome, a genetic disorder related to the sex chromosomes.Here, we describe the first reported use of the QIAGEN Investigator® Argus X-12 kit for characterization of X-chromosomal STR loci to potentially identify a case of Klinefelter syndrome. This commercially available kit is primarily used in forensic laboratories to investigate kinship relations and for paternity testing in alleged father/daughter cases. Results of the X chromosome DNA profiling from the victim’s disposable drinking cup and reference samples revealed two alleles at various X-chromosomal STR loci. Moreover, this kit can also amplify a Y chromosome specific sequence (AMEL-Y), and the results indicated that this sample actually originated from a male. Evidence of two X chromosomes in the victim's DNA suggested that he was likely to have Klinefelter syndrome. In this case report, we propose the use of the QIAGEN Investigator® Argus X-12 kit as a practical forensic tool for the detection of potential genetic syndromes related to the sex chromosomes, which can affect test results and, at times, make them difficult to interpret. We also aim to increase awareness within the forensic science community regarding the existence of genetic anomalies, which should be considered when analyzing DNA profiles.     

心可舒片对非ST段抬高型急性冠脉综合征PCI患者非靶病变粥样硬化斑块的影响

摘要：目的：研究心可舒片联合西药对非ST段抬高型急性冠脉综合征PCI患者非靶病变斑块的影响。方法：纳入60例非ST段抬高型急性冠脉综合征PCI患者,采用随机数字表法随机分为对照组30例和治疗组30例。对照组予以常规西药,治疗组在此基础上联合心可舒片。对比两组病人的血脂、炎症因子以及非靶病变血管粥样硬化斑块的IVUS影像学指标。结果：治疗后,两组总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白（LDL-C）、 超敏C反应蛋白（hs-CRP）、白介素-6（IL-6）、斑块负荷（PB）指标均降低,最小管腔面积（MLA）及高密度脂蛋白（HDL-C）指标上升,且治疗组各项指标比对照组更优异（P ＜0.05）。结论：心可舒片能显著改善患者血脂、炎症因子以及非靶病变血管粥样硬化斑块的血管内超声（IVUS）影像学指标。