产蛋下降综合征病毒PCR检测方法的建立

根据已报道的腺病毒DNA序列 ,设计合成了 1对核苷酸引物 ,引物间隔约 63 0bp。用该引物对EDS76病毒内蒙古分离株 (N3 ,N4 )和参考株 (AV 1 2 7)核酸进行聚合酶链式反应 (PCR)。结果 ,均扩增出了约 63 0bp的特异性DNA片段。所建立的PCR方法可检出 1 0 0pg的样品模板。结果表明 ,此PCR方法是检测EDS76病毒的一种简便快速、特异性强和灵敏度高的方法     

猪繁殖与呼吸综合征疫苗的研究进展

简要介绍了国内外猪繁殖与呼吸综合征弱毒疫苗、灭活疫苗和基因工程疫苗的研究进展,分析了猪繁殖与呼吸综合征疫苗研制中存在的问题和未来发展趋势,以便兽医药科技工作者全面了解猪繁殖与呼吸综合征疫苗的研发及使用现状,进而指导临床应用.     

Death Caused by Hepatodiaphragmatic Interposition of the Colon

Hepatodiaphragmatic interposition of the colon is a rare, usually asymptomatic, anomaly and is typically an incidental radiologic finding. There are few cases in the literature describing the symptomatic form of the condition, known as Chilaiditi syndrome. In some cases, it may be accompanied by various severe complications. If symptoms are present, usually conservative treatment is given. However, conservative treatment only addresses the symptoms but does not prevent their recurrence and possible complications. Our present report shows that this anomaly may not only cause symptoms, but may also progress and cause severe complications, in our case—megacolon leading to right heart failure and, ultimately, death. To date, however, there have been no literature reports of death caused by colonic interposition. Therefore, it is important to draw attention to the importance of this anomaly and its appropriate diagnosis and treatment to ensure the most favorable patient outcomes.     

原发性高血压中医证型分布规律及其危险因素研究

目的 探讨原发性高血压中医证型的分布规律及其与危险因素的相关性。方法 收集290例原发性高血压患者的一般资料,采用无序多分类Logistic回归分析原发性高血压中医证型与性别、年龄、体质量指数、饮食偏嗜、吸烟史、饮酒史、高血压病家族史的相关性。结果 原发性高血压中医证型以痰湿壅盛证为主。单因素分析结果显示,原发性高血压中医证型与年龄、饮酒史、高血压家族史及饮食偏嗜具有相关性（P<0.05）。无序多分类Logistic回归分析显示,以阴阳两虚型为对照组,年龄≤65岁（P<0.05,OR=29.328）和高血压病家族史（P<0.05,OR=9.499）与肝火亢盛型相关;年龄≤65岁（P<0.05,OR=9.241）和饮食偏咸（P<0.05,OR=4.840）与痰湿壅盛型相关;年龄≤65岁（P<0.05,OR=10.304）和高血压病家族史（P<0.05,OR=8.312）与阴虚阳亢型相关。结论 痰湿壅盛型是原发性高血压的主要证型,饮食偏咸是痰湿壅盛型的危险因素;家族史是肝火亢盛型、阴虚阳亢型的危险因素。     

大鼠脾虚证与血清甲状腺激素及下丘脑、胸腺细胞核T3受体关系

目的：探讨脾虚证与血清甲状腺激素及下丘脑、胸腺细胞核T3受体的关系。方法：成年SD大鼠32只了随机分为4组（每组8只）：A组（正常组）、B组（脾虚组）、C组（治疗组）、D组（自复组）。采用放射配基分析法,测定各组大鼠血清T3、T4、FT3、FT4、 γ－T3 及下丘脑、胸腺细胞核T3受体的含量。结果：B、D组大鼠血清T3、T4、FT3、FT4含量均较A、C组低,有显差异（P＜0.05或P＜0.01）;各且间血清γ－T3 含量无显性差异;B、D组下丘脑、胸腺细胞T3受体含量较A、C组低,有显差异（P＜0.01）。结论：血清甲状腺激素及下丘脑、胸腺细胞核T3受体的水平降低,可导致甲状腺激素生物效应低下,经由神经内分泌免疫调节环路,削弱免疫功能,这可能是脾虚证的一种重要病机。     

大黄泄浊颗粒保留灌肠对非透析慢性肾病湿热证患者血清结缔组织生长因子及肝细胞生长因子的影响

目的观察非透析慢性肾病湿热证患者血清结缔组织生长因子(connective tissue growth factor,CTGF)和肝细胞生长因子(hepatocyte growth factor,HGF)水平及大黄泄浊颗粒保留灌肠对其干预作用。方法将63例非透析慢性肾病3~4期湿热证患者随机分为治疗组32例和对照组31例,并设正常组20例。治疗组和对照组均给予基础治疗,治疗组加用大黄泄浊颗粒保留灌肠,每日1次,疗程8周。观察两组临床疗效、血尿素氮(blood urea nitrogen,BUN)、血清肌酐(serum creatinine,SCr),以及治疗前后血清CTGF、HGF,并与正常组进行比较。结果治疗组临床疗效和中医证候疗效均显著优于对照组(P0.05)。治疗后治疗组BUN较治疗前显著降低(P0.05),SCr呈降低趋势(P0.05);对照组治疗后BUN较治疗前呈降低趋势(P0.05),SCr较治疗前显著升高(P0.05)。治疗组和对照组治疗前CTGF、HGF水平显著高于正常组(P0.05);治疗后治疗组和对照组血清CTGF、HGF水平均显著降低(P0.05);治疗组治疗后HGF和CTGF下降值大于对照组,差异无统计学意义(P0.05)。结论大黄泄浊颗粒保留灌肠改善非透析慢性肾病湿热证患者的机制与降低血清CTGF、HGF水平有关。     

中药外用治疗膝关节骨性关节炎的临床观察

目的:观察中药外用治疗膝骨性关节炎的疗效.方法:将58例膝骨性关节炎患者随机分为治疗组(35例)和对照组(23例),治疗组采用中药外敷和熏洗,对照组内服芬必得,治疗6周后按JOA的膝骨性关节炎治疗效果判定标准进行打分评估.结果:治疗组优良率为85.7%,对照组优良率为82.6%.治疗组在改善关节屈曲角度及强直挛缩方面优于对照组(P<0.05),且没有内服药的不良反应.结论:中药外治法是非手术治疗膝骨性关节炎的一种可行方法.     

断奶猪多系统衰弱综合征血清抗体检测

从北京、河北、山东、天津、江西、吉林、河南7省(市)22个猪群采集各类猪血清样品559份,用猪圆环病毒Ⅱ型(PCVⅡ)克隆化特异性表达抗原包被反应板,采用ELISA检测断奶猪多系统衰弱综合征(PMWS)抗体.结果,20日龄未断奶仔猪阳性率为0(0/58)、1～2月龄断奶仔猪阳性率为16.5%(23/139),后备母猪阳性率为43.3%(61/141),经产母猪阳性率为85.6%(107/125),肥猪阳性率为51.0%(49/96),559头猪总阳性率为42.9%(240/559).检测临床有疑似PMWS症状的4～5月龄生长肥育猪6头,结果均为阳性;检测临床有疑似PMWS症状的1～2月龄仔猪9头,结果均为阴性.     

Mechanisms of Lethal Cerebrovascular Accidents in Turner Syndrome

A case of intracerebral hemorrhage in Turner syndrome is reported with an analysis of possible causes of cerebrovascular accidents in this condition. A 42‐year‐old woman with known Turner syndrome died soon after hospital admission having been found unconscious at her home address. At autopsy, she showed typical features of Turner syndrome with short stature, webbing of the neck, underdeveloped breasts, and an increased carrying angle of the arm. Death was due to a large left‐sided intracerebral hemorrhage extending from the left basal ganglia into the white matter of the frontal lobe and lateral ventricle. Cases of unexpected death in Turner syndrome may arise from occult cerebrovascular accidents which may be hemorrhagic or nonhemorrhagic. Associated features include hypertension, vascular malformations, accelerated atherogenesis, cystic medial necrosis, and moyamoya syndrome. The possibility of Turner syndrome should be considered in cases where there has been a lethal cerebrovascular event in a younger woman.     

The Institution of a Standardized Investigation Protocol for Sudden Infant Death in the Eastern Metropole,Cape Town,South Africa,,

The rate for the sudden infant death syndrome (SIDS) in Cape Town, South Africa, is estimated to be among the highest in the world (3.41/1000 live births). In several of these areas, including those of extreme poverty, only sporadic, nonstandardized infant autopsy, and death scene investigation (DSI) occurred. In this report, we detail a feasibility project comprising 18 autopsied infants with sudden and unexpected death whose causes of death were adjudicated according to the 1991 NICHD definitions (SIDS, n = 7; known cause of death, n = 7; and unclassified, n = 4). We instituted a standardized autopsy and infant DSI through a collaborative effort of local forensic pathology officers and clinical providers. The high standard of forensic investigation met international standards, identified preventable disease, and allowed for incorporation of research. We conclude that an effective infant autopsy and DSI protocol can be established in areas with both high sudden unexpected infant death, and elsewhere. (SUID)/SIDS risk and infrastructure challenges.