A fatal dichlorvos poisoning: concentrations in biological specimens

Abstract:  A 54-year-old man was found dead with a bottle containing a brownish fluid near him. A toxicological screening was carried out in blood, urine, and stomach content. Only dichlorvos (2,2 dichlorovinyl O-O dimethylphosphate or DDVP) was found. A simple and rapid method, using DDVP-D₆ as an internal standard, was developed for the determination of DDVP by gas chromatography/mass spectrometry (GC/MS). The method was linear from 1 to 10 mg/L. Intraday and interday precisions were all <15%. DDVP concentration in cardiac blood was approximately four times higher than in peripheral blood. A high concentration was found in the heart showing a cardiac tropism of DDVP, kidney and lung concentrations being much lower. No DDVP was found in liver. DDVP stomach content was 38 g. The amount presumed ingested was 82 g, c . 1000 mg/kg of body. The oral LD₅₀ for DDVP ranges between 20 and 1090 mg/kg in animals but is not known for humans.     

Thiodicarb and methomyl tissue distribution in a fatal multiple compounds poisoning

Abstract:  Thiodicarb is a nonsystemic carbamate insecticide whose acetylcholinesterase activity is related to its main methomyl degradation product. A 40-year-old woman was found dead in her car. Empty packages of medicines and an open bottle of Larvin^® containing thiodicarb were found near her body. No signs of violence nor traumatic injuries were noticed upon autopsy, and police investigations strongly suggested a suicide. Systematic toxicological analysis performed on postmortem specimens revealed the presence of various sedatives, hypnotics, and antipsychotic drugs in blood, urine, and gastric content. Some of the compounds identified were determined at blood concentrations well above the known therapeutic concentrations: zolpidem (2.87 mg/L), bromazepam (2.39 mg/L), nordazepam (4.21 mg/L), and levopremazine (0.64 mg/L). Specific analysis of thiodicarb and of its methomyl metabolite was then performed on all fluids and tissues collected during autopsy by liquid chromatography ion trap tandem mass spectrometry (LC-MS-MS). The anticholinesterase capacity of blood, urine, and gastric content collected at autopsy was 83%, 82%, and 32%, respectively (normal value: 0%). The presence of thiodicarb in the bottle found near the body corroborates the hypothesis of an intake of that compound. Although thiodicarb was only detected in gastric content (24.3 mg/L), its methomyl metabolite was quantified in most postmortem tissues and fluids: gastric content (19.9 mg/L), peripheral blood (0.7 mg/L), urine (8.5 mg/L), bile (2.7 mg/L), liver (0.7 mg/kg), kidney (1.7 mg/kg), lung (1.5 mg/kg), brain (9.3 mg/kg), and heart (3.6 mg/kg).     

Facial soft tissue depths in craniofacial identification (part I): An analytical review of the published adult data

With the ever increasing production of average soft tissue depth studies, data are becoming increasingly complex, less standardized, and more unwieldy. So far, no overarching review has been attempted to determine: the validity of continued data collection; the usefulness of the existing data subcategorizations; or if a synthesis is possible to produce a manageable soft tissue depth library. While a principal components analysis would provide the best foundation for such an assessment, this type of investigation is not currently possible because of a lack of easily accessible raw data (first, many studies are narrow; second, raw data are infrequently published and/or stored and are not always shared by some authors). This paper provides an alternate means of investigation using an hierarchical approach to review and compare the effects of single variables on published mean values for adults whilst acknowledging measurement errors and within-group variation. The results revealed: (i) no clear secular trends at frequently investigated landmarks; (ii) wide variation in soft tissue depth measures between different measurement techniques irrespective of whether living persons or cadavers were considered; (iii) no clear clustering of non-Caucasoid data far from the Caucasoid means; and (iv) minor differences between males and females. Consequently, the data were pooled across studies using weighted means and standard deviations to cancel out random and opposing study-specific errors, and to produce a single soft tissue depth table with increased sample sizes (e.g., 6786 individuals at pogonion).     

非法出售、私赠文物藏品罪研究

非法出售和私赠文物藏品罪侵犯了国家对文物藏品的管理活动和对国有单位所收藏文物的所有权 ,非国有单位或单纯个人不构成本罪。为正确认定本罪 ,应划清本罪与近似犯罪的区别。     

脑组织与其相关分析推断死亡时间的研究进展

推断死后间隔时间(postmortem interval,PMI)一直是法医学鉴定中需要解决的重要问题之一.可用于推断PMI的尸体检材有很多种,脑组织因其独特的解剖学位置与生理功能,是推断PMI的重要检材之一.本文从三个方面将根据脑组织推断PMI的方法进行综述,包括了通过脑组织的温度、形态特点等尸体现象推断PMI;通过...     

Addition of an adipose marker to the organ tissue identification mRNA assay

At the Netherlands Forensic Institute, mRNA profiling for the purpose of organ tissue identification is frequently requested in casework. The previous version of the assay (denoted Organtyper) lacked markers for identifying adipose tissue, while this was occasionally questioned in casework. Here, three potential adipose markers are examined before incorporating one into the final Organtyper assay. The added value of the adipose marker becomes clear from looking at casework results.     

Comparing the Life-Cycle Costs of New Construction and Acquisition-Rehab of Affordable Multifamily Rental Housing

The cost of producing multifamily affordable housing may differ based on development type. Past attempts to compare the costs of producing multifamily housing through new construction or acquisition-rehab have been limited by an inability to adjust for variations in unit quality among different projects. The authors overcome this challenge by estimating the costs of developing and maintaining a property over a 50-year life cycle. Applying this approach to a convenience sample of 269 properties, the authors find new construction associated with life-cycle costs that are 25% to 45% higher than those of acquisition-rehab.     

县域社会违建执法的组织形态与治理绩效辨析

县域社会违建执法需要一定的组织载体,但在不同农村类型中,体制层面执法资源配置不均和社会层面违建个体情境差异共同塑造了不同类型的政府执法组织,并产生不同治理绩效。在城中村,正式化执法组织具备常规执法权且执法密度大,虽能够强化执法效果,但在执法过程中始终存在政府与征拆居民的利益博弈;在城郊村,正式化与半正式化相结合的执法组织有助于强化政府执法力量和权威,但受信息制约和人情关系影响容易产生灰色利益空间,进而侵蚀执法目标;在远郊村,半正式化执法组织本身没有专业执法权,政府常在一定时期内通过组织动员手段来补强执法力量,但执法绩效具有不稳定性。三类农村违建执法组织的治理绩效差异,反映出国家法律在县域城乡社会落地的非均质性。     

《民法典》背景下环境保护禁止令的法教义学展开——基于人格权禁令制度的考察

《最高人民法院关于生态环境侵权案件适用禁止令保全措施的若干规定》对于建立健全环境保护禁止令制度具有重要意义。不过,以旨在确保本案诉讼的行为保全制度为基础的环境保护禁止令,并不能完全实现及时制止生态环境损害的目标。《民法典》第997条规定的人格权禁令兼具实体基础与程序规则的功能,具有独立性。基于环境权益具有一定的人格权属性,人格权禁令可适用于制止正在实施或即将实施的生态环境损害行为。人格权禁令制度为基础的环境保护禁止令,其保护范围宜限于物质性环境人格权,适用标准需引入环境保护法禁止性规范;在程序构建方面,有必要区分环境私益保护的一般禁令程序与环境公益保护的紧急禁令程序,同时设置裁执一体化的快速实施程序。     

肌红蛋白降解与人体死亡时间的相关性

目的检测人体死亡后骨骼肌中肌红蛋白的降解水平,探讨其与死亡时间的关系。方法分别于人体死后0、4、8、12、24、36、48、60、72h取骨骼肌,置于室温25℃,在不同时间点抽提蛋白质,用Western Blot方法检测肌红蛋白,随后利用生物电泳图像分析软件分析。结果肌红蛋白随死后时间逐步降解。结论肌红蛋白降解的检测可用于推断死亡时间。