α-纤维蛋白原基因多态性与肺动脉栓塞相关性初探

目的探讨α-纤维蛋白原Thr312Ala基因的多态性分布与肺动脉栓塞的相关性。方法选取尸检已确诊的肺动脉栓塞病例的甲醛固定心肌组织14例和正常健康人群外周血56例,采用改良酚-氯仿法和Chelex100法分别提取心肌组织和外周静脉血基因组DNA,用PCR-RFLP法检测α-纤维蛋白原基因Thr312Ala多态性,并分析其与肺动脉栓塞的关联性。结果α-纤维蛋白原Thr312Ala基因的各基因型在肺动脉栓塞组与对照组比较差异具有显著性意义（P〈0.05）;各等位基因在两组间比较差异具有显著性意义（P〈0.05）;A等位基因与PE呈负相关（RR=0.475,P〈0.05）;G等位基因与PE呈正相关（RR=5.818,P〈0.05）。结论α-纤维蛋白原基因Thr312Ala位点G等位基因与肺动脉栓塞有正关联,肺动脉栓塞死亡者中GA、GG基因型分布频率较高,本文数据可为相关研究提供参考。     

Evaluation of the Abandoned Shipwreck Act of 1987: Impact and Challenges for Preserving Underwater Cultural Heritage

In the demand of regulatory policy to control salvage of shipwrecks and protect the nation's underwater cultural heritage, the Abandoned Shipwreck Act of 1987 allowed the exception of the admiralty law to give states authority and management of abandoned shipwrecks for the purposes of preservation and recreation. Due to the lack of its regulatory power and vague standards, the Act has been harshly criticized for being ineffectual in resolving conflicts between commercial salvors, and states or protecting illicit salvage. This research will examine the purpose and impact of the policy under the framework of the National Historic Preservation Act, which restrained options in the policymaking. While acknowledging its limitations, this research finds that the Act enabled the inclusion of historic shipwrecks in the nation's preservation system, allowed funding, and promoted its use for public benefits. Studying the Act in the policy context of the U.S. historic preservation can promote the rightful understanding of its policy limitations as well as impact, and support feasible policy improvement.     

生态维护之文化剖析

本文以乌江中上游、清水江流域、麻山石漠化山区三个地区的民族田野调查资料为基础,证明生态失衡并非相关民族文化正常运作的结果,而是族际关系失衡或相关文化转型而诱发的灾变。进而指出生态维护的内容分为泛化生态维护和具体生态维护两个方面,二者内容和对策理当有别。因而,维护人类赖以生存的自然生态,不能单凭政治、经济、法律手段,只有依靠多元文化并存建构起来的稳定文化制衡格局,或凭借文化要素嫁接建构起来的族内文化要素制衡,才能获得维护生态环境正常运作的持续动力。     

Distribution of Venlafaxine,O‐desmethylvenlafaxine,and O‐desmethylvenlafaxine to Venlafaxine Ratio in Postmortem Human Brain Tissue

Venlafaxine (VEN) and its metabolite O‐desmethylvenlafaxine (ODV) inhibit reuptake of serotonin and norepinephrine. This study examines whether VEN is differentially distributed in postmortem brain and examines relationships between brain and femoral blood concentrations from donors prescribed VEN for treatment of depression. Using high‐pressure liquid chromatography‐ultraviolet detection, VEN and ODV concentrations were measured in temporal, occipital, and cerebellar cortex of six postmortem brains. The ODV/VEN ratio was calculated as a relative measure of drug metabolism within each region where higher ratios indicated a greater conversion of VEN to ODV. Compared to the other regions examined, the cerebellum showed decreased VEN (p = 0.056), ODV (p = 0.006), and ODV/VEN (p = 0.027) ratios. In parts per million, VEN was higher in temporal and occipital cortex, but not cerebellum, as compared to femoral blood concentration. These observations suggest that VEN and ODV are differentially distributed in the brain, and metabolism of VEN to ODV may vary across brain regions.     

我国银行不良资产处置瓶颈分析

该文通过对L地区资产包案例的解读，从权利核与价值面立体探析我国银行不良资产的处置瓶颈，即权利保护瓶颈核上实体与程序缺位，价值实现瓶颈面上应然与实然错位，提出不良资产处置以资产包价值最大化为中心，以资产权利和资产价值为主线，完善资料整合、信息整合、技巧整合、资源整合和关系整合，协调项目内外部关系的思路，充分运用法律手段，有效运用非法律手段。     

关于完善我国仲裁中财产保全制度的几点建议

本文针对我国仲裁中财产保全制度的不足提出以下完善建议 :赋予仲裁庭决定财产保全的权力 ,全面确立仲裁前财产保全制度 ,改进仲裁中财产保全制度。     

我国民事诉讼保全制度的现状及发展

民事诉讼保全制度是民事案件审理中的一项重要的法律制度。目前我国民事诉讼保全制度还存在一些尚需商榷之处,如对于当事人提出保全申请,没有明确条件限制,以致当事人滥用申请;数额标准在司法实践中难以执行,应当修改法律规定。     

环丙沙星在霉形体性肺炎猪体内的组织药代动力学研究

将21头健康杜洛克×长白×大白杂交猪复制成霉形体性肺炎模型后,分别肌肉注射环丙沙星(5.0 mg/kg)进行组织及血浆药代动力学研究。结果显示,环丙沙星在感染猪肺中的浓度-时间数据适合一级吸收二室开放模型,外观正常肺与外观实变肺的主要参数分别是:t1/2Ka为0.19和0.46 h,t1/2α为2.68和2.52 h,t1/2β为8.12和6.87 h,tmax为1.10和1.47 h,Cmax为6.00和4.46μg/g,AUC为38.65和33.33mg/(L.h),Tcp(ther)为57.10和50.59 h。气管、支气管组织中的药物浓度-时间数据适合一级吸收一室开放模型,主要参数分别是:t1/2Ka为0.56和0.38 h,t1/2ke为5.68和5.67 h,tmax为2.08和1.60 h,Cmax为3.73和2.62μg/g,AUC为39.44和26.10 mg/(L.h),Tcp(ther)为50.60和47.16 h。表明,环丙沙星对霉形体性肺炎猪呼吸道组织具有良好的穿透性,组织药物浓度明显高于血浆药物浓度,且消除缓慢。     

GC／MS法分析被焚烧人体内脏组织中的氯氮平

目的建立GC/MS分析被焚烧人体内脏组织中氯氮平的方法。方法采用试管超声提取、三氯甲烷浸提苯溶解、过柱净化的方法,以岛津QP5050ADB-5MS石英毛细柱分析结果。结果成功检出被焚烧人体内脏组织中氯氮平成分。结论该方法操作简单,准确可靠,适用于中毒及临床药物观察。