Differences among species in compact bone tissue microstructure of mammalian skeleton: use of a discriminant function analysis for species identification

In order to develop an identification key for distinguishing between several mammalian species, bone structure of their compact bone tissue was analyzed using qualitative and quantitative characteristics. Ninety femora of adult male humans, pigs, cows, sheep, rabbits, and rats were studied. The average area, perimeter, minimum, and maximum diameter of 1863 Haversian canals and 1863 secondary osteons were measured using a digital image device. The observed data were first used to evaluate inter- and intraspecies diversity. After that, we applied a discriminant function analysis for differentiation of the species by these variables. Classification functions for investigated species give cross-validated correct classification rates for 76.17% of cases. This percentage value can be increased by integrating conclusions from the qualitative analysis.     

DNA指纹图在法医学鉴定中应用的研究(Ⅱ)——应用‘Myo’小卫星探针进行血斑、精斑、个体组织的个体认定

应用‘Myo’小卫星 DNA 探针,Southern 印迹杂交技术,对血斑、精斑、同一个体不同组织进行 DNA 指纹图分析,均获得清晰的图谱。同一个体的血斑与血液、精斑与精液以及不同的组织其 DNA 指纹图谱完全相同。可以根据斑痕或组织与嫌疑个体的血液或某一组织 DNA 的指纹图谱比对以做出同一认定。50μl 血液量的血斑、5μl 精液量的精斑可以获得清晰易辨的指纹图谱。五年的精斑、两年的血斑亦可做出与同源个体新鲜精液、血液完全一致的 DNA 指纹图谱。对杀人、强奸杀人、碎尸等不同案件的血痕、精斑、不同组织碎块进行了 DNA 指纹图检验,均做出了正确的个体认定。本方法的应用为我国法医物证检验提供了新的分析手段,使个体认定得以实现。     

The Human Tissue Act 2004: Innovative Legislation – Fundamentally Flawed or Missed Opportunity?

In November 2004 Royal Assent was finally given to the Human Tissue Act which was passed in response to the organ retention scandals at Alder Hey hospital and other hospitals around the UK. At first glance it may appear to set many of the concerns raised in the Bristol Interim Inquiry and Alder Hey Inquiry to rest. But on closer inspection a more complex position emerges. The paper examines the extent to which it can be regarded as a groundbreaking piece of legislation clarifying a notoriously uncertain area of law or whether in the long term it will be viewed as a fundamentally flawed Act and a missed opportunity.     

民族自治地方刑事立法权的新解说

根据《宪法》第一百一十五条、第一百一十六条和《民族区域自治法》第十九条,民族自治地方的人民代表大会享有自治权,可以制定自治条例和单行条例,此称为自治立法权。根据《刑法》第九十条,民族自治地方的省、自治区的人民代表大会享有对刑法的变通或者补充权,此称为变通权。根据《立法法》第八条,犯罪和刑罚的事项,只能制定法律,此称为法律保留原则。上述规定有两个问题需要厘清:一是自治立法权和变通权之间的关系,二是变通权是否违背《立法法》的法律保留原则。二者既有相同点,又有不同之处:自治立法权的法源根据是宪法,而变通权的法源根据首先在于法律(还可能包括行政法规),其次在于宪法。在法律的层次内,二者可以是统一的,在行政法规的层次内,二者是分离的。变通权的规定并不违反法律保留原则,原因是根据宪法规定,自治立法权中包含了变通权,此种变通权在法律的授权内具有独立的意义。     

广泛软组织挫伤后器官形态学的研究

目的探索法医病理学诊断广泛软组织挫伤、挤压综合征致死案例的形态学改变。方法采用大鼠广泛软组织挫伤模型和人体挤压综合征致死标本,对器官进行HE染色、肾肌红蛋白HSP免疫组化染色及电镜观察。 结果肾肌红蛋白的阳性率分别为60％、75％、95％;心肌、脑组织阳性染色率为90％;广泛软组织挫伤短期内死亡的大鼠肾电镜观察,可见多种组织细胞病理改变致使毛细血管腔狭窄、闭塞等。结论建立了较理想的大鼠广泛软组织挫伤形成的挤压综合征动物模型;为此类研究积累了具有一定实用价值的病理形态学资料。     

Anthropological facial ‘reconstruction’ – recognizing the fallacies, ‘unembracing’ the errors, and realizing method limits

Despite being practiced for over the last 200 years, facial approximation methods remain in their infancy as the soft tissue prediction methods employed have not been tested and justified. Scientific testing is the only way forward and much of it is needed. The lack of systematic scientific tests in the past has enabled many misleading notions to become established. Many of these notions appear to have arisen and been sustained as a result of practitioner biases--this is clearly evident even in the name commonly used to describe the method of building faces from skulls, for "facial reconstruction" implies everything the method is not, e.g., technical/scientific, exact, and credible. Although facial approximation methods are useful for forensic investigation (even if they do not generate identifications through true positive recognitions of the faces), the public should beware of the marketing and political ploys employed within the profession. These ploys give rise to some impressive, but unjustifiable claims--but do not just take my word for it; evaluate the evidence for yourself with disregard to the indoctrination waged by the facial "reconstruction" field in general, including that promoted by what I have had to say here. Use your own reason and intellect and see which conclusions you reach.     

间接酶标抗体染色法检测石蜡切片中鸡肾型传染性支气管炎病毒的研究

以抗鸡肾型传染性支气管炎病毒（ＮＩＢＶ）Ｔ株的鼠源超免疫血清为一级抗体,辣根过氧化物酶（ＨＲＰ）标记的羊抗鼠ＩｇＧ为二级抗体,建立了检测石蜡切片中ＮＩＢＶ抗原的间接酶标抗体染色技术。经过特异性试验和阻断性试验,表明该方法具有高度的敏感性和特异性。应用该法对人工感染ＮＩＢＶＣ９００１株的鸡气管和肾石蜡切片中的病毒抗原进行了检测,结果：气管从感染后０．５～１１ｄ,肾从感染后１～２０ｄ,均检测到病毒抗原,阳性染色主要集中于气管粘膜上皮细胞和肾小管上皮细胞浆内。     

甲醛固定及石蜡包埋组织的DNA分型及其应用

目的：建立甲醛固定及石蜡包埋组织ＤＮＡ的基因分型方法并使之应用于实际检案。方法：用Ｃｈｅｌｅｘ和有机溶剂提取法提取ＤＮＡ,以ＰＣＲ与反向探针杂交技术作ＰＭ和ＨＬＡ－ＤＱＡ１位点分型。结果：２６份包埋组织块有２４份能得到理想的分型结果。结论：本法可用于法医学个人识别和亲子鉴定     

损伤经过时间的实验研究

作者在全身麻醉及无菌条件下,通过在大白鼠背部皮肤造成的切创创口,于伤后不同时间杀死.每组中1只于处死前8小时注射秋水仙碱以观察核分裂.取损伤处皮肤,经福尔马林固定、石蜡切片、HE染色、Gorden和Sweet染色(网状纤维)、Weigert染色(弹力纤维)、PTAH染色(纤维蛋白)和Van Gieson染色,于显微镜下观察.结果发现创口逐日缩小,但其中24小时创口较12小时创口有所扩大,伤后第6～7日创口呈突击式缩小.通过组织学变化观察,作者对创口收缩现象在判断损伤时间的意义及形成机理作了讨论.     

债的相对性原则的突破与完善

债的相对性或合同的相对性一直以来为两大法系所确认,在债法特别是合同法领域中占有十分重要的地位。但是近代各国立法及判例对它已有突破,产生了为第三人利益合同、第三人侵害债权及债的保全等制度。我国也应顺应世界潮流,在民法典中坚持债的相对性原则,完善第三人利益合同制度,建立第三人侵害债权制度和细化债的保全制度。