人体组织两种简易保存法DNA抽提效果的比较研究

比较研究人体不同组织在不同保存条件下DNA抽提效果。取冷冻保存和50％乙醇固定保存的各种组织，用有机溶剂抽提法提取DNA。用50％乙醇固定保存的人体不同组织在校长时期内仍可获得与新鲜冷冻组织相近的DNA抽提效果，经卡方检验二者无明显差异（X2≤0．21，P≥0．995）。乙醇保存法简便易行，DNA产量高，效果好，适用于各种特殊情况下的组织保存。     

多发性软组织挫伤后对肺及其他脏器的影响

目的观察多发性软组织挫伤后短时间内死亡案例的脏器病理学改变。方法应用常规组织学及免疫组织化学染色技术进行光学显微镜检查。结果光镜检查见肺组织毛细血管扩张充血，白细胞集聚，肺组织散在片状出血、灶状坏死及透明膜形成；心脏间质血管扩张充血，点灶状纤维溶解；脑组织充血水肿，肝脾充血。免疫组化发现肺泡腔及部分血管内纤维蛋白染色阳性，部分脾脏血管内及肾髓质集合管中肌红蛋白染色阳性。结论本研究结果提示多发性软组织挫伤后短时间内死亡的死因为成人呼吸窘迫综合征（ARDS）合并多脏器功能衰竭。     

福尔马林固定石蜡包埋组织中DNA提取

由于甲醛介导的DNA损伤和石蜡对DNA提取的阻碍作用,使得常规DNA提取方法很难从福尔马林固定石蜡包埋组织(FFPET)中获取高质量的DNA。近年来,众多学者的研究表明,通过改良预处理方法,优化蛋白酶K消化作用,简化DNA提取步骤,纯化DNA提取物等,可以有效提高FFPET中提取的DNA质量,为FFPET的DNA分析奠定了基础。     

论我国死刑制度立法理念及其完善

本文首先介绍了死刑制度及其历史沿革,通过死刑存废之争,以及各国立法例的探讨及法律发展,指出中国现阶段不宜废除死刑,但应通过立法、司法等限制,严格控制死刑的适用范围,并对中国刑事法的发展提出立法预测———中国刑事法人道化。     

Evaluation of Commercial‐off‐the‐Shelf Materials for the Preservation of Bacillus anthracis Vegetative Cells for Forensic Analysis,

Environmental surface sampling is crucial in determining the zones of contamination and overall threat assessment. Viability retention of sampled material is central to such assessments. A systematic study was completed to determine viability of vegetative cells under nonpermissive storage conditions. Despite major gains in nucleic acid sequencing technologies, initial positive identification of threats must be made through direct culture of the sampled material using classical microbiological methods. Solutions have been developed to preserve the viability of pathogens contained within clinical samples, but many have not been examined for their ability to preserve biological agents. The purpose of this study was to systematically examine existing preservation materials that can retain the viability of Bacillus anthracis vegetative cells stored under nonpermissive temperatures. The results show effectiveness of five of seventeen solutions, which are capable of retaining viability of a sporulation deficient strain of B. anthracis Sterne when stored under nonrefrigerated conditions.     

组织切片DNA提取的探讨

在医患关系纠纷中,病理组织切片经常被作为至关重要的证据,但对它的检验是目前法医DNA检验的难点。因样本制作固定的时间、保存环境等不同因素的影响,病理组织切片DNA检出率并不高。本文从实际检案角度出发,详细介绍了病理组织切片DNA提取的过程,并分析了其可能影响因素。     

Facial Soft Tissue Thickness Among Three Skeletal Classes in Adult Pakistani Subjects

Different facial reconstruction methods rely on the average facial soft tissue thickness values provided in previous studies. Facial soft tissue thickness is influenced by the age, sex, and ethnicity of the individual. The aim of the present study was to determine facial soft tissue thickness of adult Pakistani subjects with different facial morphology. A total of 166 subjects were categorized into three skeletal classes (based on convex, straight, or concave facial profile) employing the classification system used in orthodontics. Facial soft tissue thickness was determined at ten midline points on lateral cephalograms. Significant differences in facial soft tissue thickness were present at glabella, labrale superius, stomion, and labiomentale in males and at labrale superius, labrale inferius, labiomentale, and pogonion in females among different skeletal classes. The current study suggests that the skull morphology-related variations in facial soft tissue thickness should be considered during facial reconstruction to achieve accurate results.     

Psychometric Properties of the Trauma and Post-Trauma Well-Being Assessment Domains of the North Carolina Family Assessment Scale for General and Reunification Services (NCFAS G+R)

Two new domains of the NCFAS-G+R, trauma and post-trauma well-being, were tested for reliability and validity in relation to previous NCFAS-G+R domains. Three family preservation programs provided case level data on 170 in-home service families over six months. Domains were tested for reliability using Cronbach's alpha, factor structure was explored, and convergent validity was examined through correlations of new scale items with domain ratings on the NCFAS-G+R. Assessment ratings were cross tabulated with practice and outcome variables. Reliability of the new domains was established with Cronbach's alphas of .811 and .905, respectively, factor structure was confirmed, new scale items and domains correlated predictably and significantly with other NCFAS domains. Outcome variables were influenced by trauma assessment ratings. Displaying good psychometric properties, the trauma-focused assessment domains hold promise for assisting child welfare practitioners assess trauma symptomology, and post-trauma well-being following services.     

人格权侵害禁令的程序实现

人格权侵害禁令是人格权侵害领域中预防性责任形式的快速实现机制,其独立于先予执行和普通的行为保全,是不必然伴随诉讼程序、具有非暂时性和非保全性的人格权独立保护制度。根据此种实体法功能,禁令的程序模式在价值上要权衡程序的便利高效和当事人的程序利益保障。基于非讼程序法理的发展和现行法,虽然禁令案件是真正的诉讼案件,但仍可将其作为非讼程序的审理对象,并以非讼程序作为禁令的基本程序模式。据此,禁令程序应采用职权探知主义,建立被申请人最低限度的程序保障规则,确立非讼程序和诉讼程序之间的合理转换条件和方式,且禁令程序中的裁定不具有既判力。其他的具体程序规则也应根据非讼程序予以解释构建。     

The Microbiome Forensics Database UZH

Microbial communities in biological stains can provide valuable information to assist forensic scientists identify the body fluid/tissue present in these. As these microbial communities are characteristic of body habitats, DNA sequencing of microbes can be used to predict bodily origin. Promising predictive results have been obtained with supervised machine learning algorithms trained on bacterial abundance data from human body sites. Importantly, prediction accuracy is dependent on the training dataset, yet compiling a large and comprehensive training reference is a non-trivial issue requiring substantial efforts. Here we present a new online database and associated data-mining platform which is, to our knowledge, the first one customised for forensic scientists investigating body fluids/tissues. Our database features samples originating from ten human body sites, with selection options through an online platform. Users can download bacterial abundance as well as taxonomic data, which can then be used to train predictive models and test their accuracy. Future stages of the development of the platform will include curation of the samples to decrease potential errors in sample labelling, as well as access to an online tool to conduct exploratory analyses.