全文获取类型
收费全文 | 1230篇 |
免费 | 65篇 |
专业分类
外交国际关系 | 42篇 |
法律 | 1206篇 |
中国政治 | 11篇 |
政治理论 | 3篇 |
综合类 | 33篇 |
出版年
2024年 | 4篇 |
2023年 | 25篇 |
2022年 | 57篇 |
2021年 | 23篇 |
2020年 | 38篇 |
2019年 | 59篇 |
2018年 | 32篇 |
2017年 | 17篇 |
2016年 | 37篇 |
2015年 | 24篇 |
2014年 | 33篇 |
2013年 | 92篇 |
2012年 | 61篇 |
2011年 | 78篇 |
2010年 | 54篇 |
2009年 | 120篇 |
2008年 | 103篇 |
2007年 | 110篇 |
2006年 | 116篇 |
2005年 | 33篇 |
2004年 | 25篇 |
2003年 | 26篇 |
2002年 | 31篇 |
2001年 | 25篇 |
2000年 | 21篇 |
1999年 | 5篇 |
1998年 | 8篇 |
1997年 | 3篇 |
1996年 | 7篇 |
1995年 | 2篇 |
1994年 | 7篇 |
1993年 | 3篇 |
1992年 | 1篇 |
1991年 | 4篇 |
1990年 | 5篇 |
1989年 | 4篇 |
1988年 | 2篇 |
排序方式: 共有1295条查询结果,搜索用时 31 毫秒
101.
Bienvenue JM Duncalf N Marchiarullo D Ferrance JP Landers JP 《Journal of forensic sciences》2006,51(2):266-273
The current backlog of casework is among the most significant challenges facing crime laboratories at this time. While the development of next-generation microchip-based technology for expedited forensic casework analysis offers one solution to this problem, this will require the adaptation of manual, large-volume, benchtop chemistry to small volume microfluidic devices. Analysis of evidentiary materials from rape kits where semen or sperm cells are commonly found represents a unique set of challenges for on-chip cell lysis and DNA extraction that must be addressed for successful application. The work presented here details the development of a microdevice capable of DNA extraction directly from sperm cells for application to the analysis of sexual assault evidence. A variety of chemical lysing agents are assessed for inclusion in the extraction protocol and a method for DNA purification from sperm cells is described. Suitability of the extracted DNA for short tandem repeat (STR) analysis is assessed and genetic profiles shown. Finally, on-chip cell lysis methods are evaluated, with results from fluorescence visualization of cell rupture and DNA extraction from an integrated cell lysis and purification with subsequent STR amplification presented. A method for on-chip cell lysis and DNA purification is described, with considerations toward inclusion in an integrated microdevice capable of both differential cell sorting and DNA extraction. The results of this work demonstrate the feasibility of incorporating microchip-based cell lysis and DNA extraction into forensic casework analysis. 相似文献
102.
103.
104.
The population of the Asian elephant is being dramatically reduced due to poaching of the ivory from the male. As poaching occurs in remote forests, it often takes weeks or longer for it to be discovered and it is therefore often very difficult to determine the sex of the decomposed body. Data suggest that in the recent past, over 2000 male elephants have been poached in South India. We have developed a technique based on molecular markers to determine that the carcass is an elephant and that it is a male. Using DNA sequence information from Genbank, we have developed two primer pairs: one for the mitochondrial DNA (mtDNA) and the other for the sex-determining region of Y chromosome (SRY) gene of the Indian elephant. After PCR amplification of known elephant DNA, we found that the mtDNA was common in both males and females, whereas the SRY-specific amplicon was observed only in the male. 相似文献
105.
POPULATION: Population: Illinois Caucasian ( n =117), Illinois African American ( n =218), and Illinois Hispanic ( n =68). 相似文献
106.
Recently, in Italy, a murder and a putative sexual violence was accomplished on a child. A bodily fluids mixture on the child's underwear between the victim (female) and the suspect (male) was ascertained by short tandem repeat (STR) DNA typing and, due to the absence of seminal fluid, saliva from the suspect and urine from the child was hypothesized. In order to investigate the possibility of specifically and rapidly detecting saliva stains both alone and mixed with other bodily fluids, we used a quantitative spectrophotometric technique, named Amylase test, for the detection of alpha-amylases. We determined alpha-amylase activity and reaction kinetic curves in several samples collected from the child's underwear. In order to confirm our intuition, we first tested saliva, perspiration, and urine, singularly and in mixtures; second, several forensic stains including saliva, perspiration, urine stains, saliva/perspiration, and saliva/urine mixture stains were tested. Evaluating alpha-amylase activity values and time-course curves' behavior of alpha-amylase reactions we were able to recognize successfully, in all cases, the presence of saliva and to distinguish it specifically from other bodily fluids containing alpha-amylase. A further confirmation of our result was provided by STR DNA typing on several areas of the underwear: a clear correlation between alpha-amylases activity and male DNA was detected on all the samples evaluated. 相似文献
107.
108.
109.
110.
DNA芯片技术作为一门新兴的高科技生物技术,显示了它旺盛的生命力和迅猛的发展势头。单核苷酸多态性(SingleNucleotidePolymorphisms,SNPs)是最常见的人类基因组变异类型。它作为一种有效的人类遗传标记,在疾病相关性研究、药物基因组学、法医学、人类进化和迁移等研究中发挥了重要作用。它同DNA芯片技术结合运用也将在法医检验,尤其是亲子鉴定和个人识别中发挥重要作用。本文主要讨论了DNA芯片和SNPs的特点,以及二者联合运用于法医学的价值。 相似文献