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排序方式: 共有1304条查询结果,搜索用时 15 毫秒
121.
目的探讨中介素(IMD)对大鼠体外培养心肌细胞缺氧-复氧损伤的作用。方法用大鼠H9c2心肌细胞株制作实验模型,样本分为对照组、缺氧-复氧组(缺氧1h、复氧30min)、IMD组(缺氧-复氧前30min加入10-7mol/L IMD)。采用MTT比色法检测心肌细胞活力;透射电镜观察细胞超微结构;激光共聚焦显微镜观察并测定细胞内钙离子浓度;流式细胞术测定细胞凋亡率。结果与对照组比较,缺氧-复氧组和IMD组细胞存活率显著降低,而IMD处理组明显升高细胞存活率(P〈0.01);在形态学上,IMD预处理可明显减轻缺氧-复氧对大鼠心肌细胞的损伤;缺氧-复氧组细胞[Ca2+]i荧光强度和细胞凋亡率比对照组显著升高,IMD预处理可明显降低上述升高的比率(P〈0.01)。结论 IMD对大鼠心肌细胞缺氧-复氧损伤有一定保护作用,提高心肌细胞存活率、减轻心肌细胞钙超载和抑制凋亡是其作用途径。 相似文献
122.
Johan Duflou F.R.C.P.A. Bradley McNamara Certificate in Mortuary Practice Robert Cluney Certificate in Mortuary Practice 《Journal of forensic sciences》2014,59(1):224-225
Restoration of autopsy incisions can result in inadvertent needle stick injury to the prosector, which can be difficult to prevent even with the use of personal protective equipment such as Kevlar cut resistant gloves. We present a new technique for closure of autopsy incisions using a commonly available commercial hardware tool, a hole punch with a lever enhanced action, combined with blunt probe sewing, which results in an esthetic and leak‐proof means of restoring cadavers. This technique is especially useful in cases which may pose blood‐borne infection risks to the prosector. 相似文献
123.
Alberto Amadasi M.D. Simone Borgonovo D.V.M. Alberto Brandone B.Sc. Mauro Di Giancamillo D.V.M. Cristina Cattaneo Ph.D. M.D. 《Journal of forensic sciences》2014,59(3):712-717
The radiological search for GSR is crucial in burnt material although it has been rarely tested. In this study, thirty‐one bovine ribs were shot at near‐contact range and burnt to calcination in an oven simulating a real combustion. Computed tomography (CT) and magnetic resonance (MR) were performed before and after carbonization and compared with former analyses with DR (digital radiography); thus comparing the assistance, the radiological methods can provide in the search for GSR in fresh and burnt bone. DR demonstrated the greatest ability in the detection of metallic residues, CT showed lower abilities, while MR showed a high sensitivity only in soft tissues. Thus, DR can be considered as the most sensitive method in the detection of GSR in charred bones, whereas CT and MR demonstrated much less reliability. Nonetheless, the MR ameliorates the analysis of gunshot wounds in other types of remains with large quantities of soft tissues. 相似文献
124.
This case study involves the unexplained death of a previously healthy 30‐month‐old child. Reportedly, she was found unresponsive by her foster father following a 2‐hour nap. She was transported to the hospital and died in the emergency room of unknown causes. Blood drawn in the ER showed hemoglobin of 4.3 mg/dL. Postmortem skeletal survey, magnetic resonance imaging (MRI) of the brain, and retinal examinations were negative. The medical examiner assumed jurisdiction of the body. The autopsy showed massive retroperitoneal hemorrhage, transections of the abdominal aorta, and inferior vena cava, and complete tears of the anterior longitudinal ligament of the spine at C5/C6 and L1/L2 with diastases of the vertebral bodies at the corresponding intervertebral disk spaces. The case is believed to be extraordinary due to both the extent of injury that does not match the reported history and the mechanism of vertebral and vascular injuries. 相似文献
125.
目的研究人脑在急性乙醇中毒状态下tPA、MMP-2、MMP-9及AEG-1表达水平与乙醇浓度的关系,分析乙醇与外伤在蛛网膜下腔出血死亡机制中的作用。方法选取15例实际案例,提取脑组织进行组织病理学检查、心血进行乙醇浓度检测,用免疫组织化学法观察人脑干、大脑、小脑tPA、MMP-2、MMP-9及AEG-1表达水平。结果急性乙醇中毒外伤组和急性乙醇中毒组的脑组织肿胀。除小脑tPA外,tPA、MMP-2、MMP-9及AEG-1表达水平在饮酒者较未饮酒者在脑干、大脑、小脑均有升高(P0.05),MMP-2、MMP-9和AEG-1表达水平与心血乙醇浓度存在正相关(P0.05,r0.6)。结论急性乙醇中毒状态下人脑tPA、MMP-2、MMP-9、AEG-1表达较未饮酒者均显著增强,MMP-2、MMP-9及AEG-1表达水平与乙醇浓度存在相关性,乙醇对脑细胞有毒性作用。 相似文献
126.
目的探讨内质网应激在脂多糖(1ipopolvsaccharide,LPS)诱导的肝细胞凋亡中的作用。方法肝细胞系BRL细胞培养,用LPS、内质网应激诱导剂毒胡萝卜素(thapsigargin,TG)、内质网应激抑制剂4-苯基丁酸(4-phenylbutyricacid,4-PBA)分别或组合处理肝细胞。用M1Tr比色法检测细胞活力的变化.Heochst33258荧光染色检测细胞凋亡形态的变化,AnnexinV-FITC/PI双染流式细胞术检测细胞凋亡率.Western印迹法检测内质网应激标志蛋白GRP78和内质网应激相关的凋亡蛋白CHOP、caspase-12和激活型caspase-3的表达。结果LPS引起肝细胞活力降低、细胞凋亡率明显增加,并具有量效和时效关系,LPS诱导GRP78、CHOP、easpase-12和激活型caspase-3的表达上调:TG可引起肝细胞活力降低和细胞凋亡增加,并能加重LPS引起的肝细胞损伤;4-PBA明显抑制LPS引起的肝细胞凋亡增加。结论内质网应激拳与介导了T,PS引起的肝细胞凋亡.提示内盾网应激暑T,PS诱导肝细胞桶伤的节娶发病给径- 相似文献
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130.
Oluwaseun B. Ogunbona Michael Heninger Kyle Bradley Rachel Geller 《Journal of forensic sciences》2022,67(1):387-390
Few cases of natural sudden death presenting as an undiagnosed lymphoma have been reported in the literature, especially in adolescents. Herein we provide a report of sudden death caused by undiagnosed classic Hodgkin lymphoma (cHL). We describe an 18-year-old female who collapsed after several weeks of weight loss, decreased appetite, and dyspnea. At autopsy, a bulky mass arising in the mediastinum and neck compressed the esophagus and trachea, surrounded the great vessels, obliterated the pericardial sac, and infiltrated the myocardium. The lungs were collapsed and large pleural effusions were present. The tumor burden, which weighed at least 2710 g in aggregate, was entirely above the diaphragm. Microscopic examination of the masses showed features typical for nodular sclerosis cHL including large bands of sclerosis, numerous Hodgkin/Reed-Sternberg (HRS) cells, and an eosinophil-rich mixed inflammatory cell infiltrate. Immunohistochemical stains showed the HRS cells to be uniformly positive for CD30 and CD15 and negative for CD3, CD20, CD45, and PAX5. This case exemplifies a rare sudden natural death due to previously undiagnosed cHL in a young patient. 相似文献